Improvement of Sperm Quality of Bali Cattle by Supplementation of Crude Tannin in the Semen

Abstract

The research was conducted to increase quality of Bali cattle’s sperm. The research used 15 Bali cattle’s sperm from Lombok Barat Regency in West Nusa Tenggara (NTB) on the average of 3 years old. For normal sperm, it is divided into five treatments of crude tannin (cT), which are P0 = sperm + tris yolk (control); P1 = P0 + 2.5% cT · mL⁻¹ sperm; P2 = P0 + 5% cT · mL⁻¹ sperm; P3 = P0 + 10% cT · mL⁻¹ sperm; and P4 = P0 + 20% cT · mL⁻¹ sperm. There is treatment of the sperm stored at 15 °C and observed for 14 days (± 10% motil). Variables observed were sperm quality, motility, viability, and abnormality in upper and lower layer of crude tannin diluent. The data from observation output on semen quality were analyzed qualitatively with comparing between treatments. The output of this research showed the highest sperm quality was shown by treatment of P2 (P0 + 5% cT · mL⁻¹ sperm); on the lower layer of diluent (%) are 65.23 ± 15.21 (motility), 55.21 ± 15.19 (viability), and 4.45 ± 1.92 (abnormality); with 7 days storage and upper layer of diluent (%) are 54.28 ± 18.95 (motility), 65.67 ± 21.04 (viability), and 7.00 ± 2.06 (abnormality) with 14 days storage. The conclusion of this research is giving treatment of P2 (P0 + 5% cT · mL⁻¹ sperm) can effect to maintain sperm motility with storage 14 days.