Abstract
Bacterial wilt caused by Ralstonia solanacearum is one of the severe diseases on chili. Until now this disease is difficult to control, while these bacteria attack the xylem vessel and are unreachable by any bactericide. One group of important biocontrol agents are the plant growth-promoting rhizobacteria (PGPR). The PGPR have also the ability to increase growth and yield of plants. The aim of this research was to obtain rhizobacterial isolates, which have the ability to control bacterial wilt on chili and to increase growth and yield. Source of of rhizobacterial isolates were from healthy chili rhizosphere at endemic area of bacterial wilt diseases in two district (Agam and Solok) West Sumatera Province, Indonesia. Screening method based on in planta selection of root-colonizing bacterial isolates. This approach focuses on indirect mechanisms (systemic induced resistance). This technique has the possibility to find new, easy, and cheap biocontrol organisms. We have isolated 42 rhizobacteria from healthy chili’s rhizosphere. Only 17 rhizobacterial isolates could increase seedling growth of chili compared to control plants; the rest of isolates reduced growth of chili seedlings. There were no bacterial wilt symptom on 13 rhizobacterial isolate introduced chilies and lower infection (33.3%) on two rhizobacterial isolate introduced chilies, compared with control plants (100% wilt and die). We have obtained also two rhizobacterial isolates which could control bacterial wilt diseases and increase growth of chili. They were RZ.2.1.AG1 identified as Bacillus cereus strain INACH001 and RZ.1.3.AP1 as Bacillus subtilis strain BSn5.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Lin, C.H., Wang, J.F.: Phosphorous acid salt: a promising chemical to control tomato bacterial wilt. http://www.spipm.cgiar.org/c/document_library/get_file?p_l_id= 17830&folderId= 18484&name=DLFE-3821.pdf Technical a Innovation Brief (2011). Acessed 15 Jan 2015
Swanson, J.K., et al.: Behavior of Ralstonia solanacearum race 3 biovar 2 during latent and active infection of geranium. Phytopathology. 95, 136–143 (2005)
Wang, J.F., et al.: Worldwide evaluation of an international set of resistance sources to bacterial wilt in tomato. In: Prior, P., Allen, C., Elphinstone, J. (eds.) Bacterial wilt disease, pp. 269–275. Springer, Berlin (1998)
Lin, C.-H., et al.: Application of a preliminary screen to select locally adapted resistant rootstock and soil amendment for integrated management of tomato bacterial wilt in Taiwan. Plant Dis. 92, 909–916 (2008)
Nguyen, M.T., Ranamukhaarachchi, S.L.: Soil–borne antagonists for biological control of bacterial wilt disease caused by Ralstonia solanacearum in tomato and pepper. J. Plant Pathol. 92(2), 395–406 (2010)
Nakkeeran, S., et al.: Induction of plant defence compounds by Pseudomonas chlororaphis PA23 and Bacillus subtilis BSCBE4 in controlling damping-off of hot pepper caused by Pythium aphanidermatum. Biocontrol Sci. Tech. 16(4), 403–416 (2006)
Shekhawat, G.S., et al.: Possibilities of biological management of potato bacterial wilt with strains of Bacillus sp., B. subtilis, P. fluorescens and Actinomycetes. In: Hartman, G.L., Hayward, A.C. (eds.) Bacterial Wilt, pp. 327–330. ACIAR Proceedings, 45: Australian Center for International Agricultural Research. Canberra, Australia (1993)
Lwin, M., Ranamukhaarachchi, S.L.: Development of biological control of Ralstonia solanacearum through antagonistic microbial populations. Int. J. Agric. Biol. 8(5), 657–660 (2006)
Hoang, H.L., et al.: Identification of the endophytic bacterial isolates and their in vitro and in vivo antagonism against Ralstonia solanacearum. J. Fac. Agric. Kyushu. U. 49, 233–241 (2004)
Messiha, N.A.S., et al.: Stenotrophomonas maltophilia: a new potential biocontrol agent of Ralstonia solanacearum, causal agent of potato brown rot. Eur. J. Plant Pathol. 118, 211–225 (2007)
Toyota, K., Kimura, M.: Growth of the bacterial wilt pathogen Pseudomonas solanacearum introduced into soil colonized by individual soil bacteria. Soil Biol. Biochem. 28, 1489–1494 (1996)
Ciampi-Panno, L., et al.: Biological control of bacterial wilt of potatoes caused by Pseudomonas solanacearum. Am. Potato J. 66, 315–332 (1989)
Whipps, J.M.: Microbial interaction and biological in the rhizosphere. J. Exp. Bot. 52, 487–511 (2001)
Egamberdieva, D.: Plant growth promoting properties of Rhizobacteria isolates from what and pea grown in loamy sand soil. Turk. J. Biol. 32, 9–15 (2008)
Nezarat, S., Gholami, A.: Screening plant growth promoting rhizobacteria for improving seed germination, transplant growth and yield of maize. Pakistan J. Biol. Sci. 12(1), 26–32 (2009)
Habazar, T., et. al.: In planta technique, for screening rhizobacteria as biocontrol agents of bacterial wilt on ginger. Paper present at 1st SIB International Conference on Biodiversity, Solo 23–24 July 2011
Habazar, T., et al.: In planta technique, for screening rhizobacteria as biocontrol agents against bacterial plant pathogens. Paper presented in: International Seminar of Indonesian Society of Microbiology (ISISM), Bogor. 4–7 October 2012
Bhadbhade, B.J., et al.: Biomineralization of an organophosphorus pesticide, Monocrotophos, by soil bacteria. J. Appl. Microbiol. 93(2), 224–234 (2002)
Winstead, N.N., Kelman, A.: Inoculation techniques for evaluating resistance to Pseudomonas solanacearum. Phytopathology. 42, 628–634 (1952)
Vidhyasekaran, P., et al.: Development of a powder formulation of Pseudomonas fluorescens for control of rice blast. Plant Pathol. 46, 291–297 (1997)
Marmur, J.: A procedure for isolation of deoxyribonucleic acid from micro-organisms. J. Mol. Biol. 3, 208–218 (1961)
Kawasaki, H., et al.: Is intracytoplasmic membrane structure a generic criterion: it is not parallel to phylogenic interrelationship among photosynthetic purple non–sulphur bacteria. Arch. Microbiol. 160, 358–362 (1993)
Yamada, Y., et al.: Asaia bogorensis gen. Nov. Sp. Nov. An. Usual acetid acid bacterium in the α–Proterobacteria. Int. J. Syst. Evol. Microbiol. 50, 823–829 (2000)
Katsura, K., et al.: Asaia siamensis sp. Nov., am acetic acid bacterium in the α–Proterobacteria. Int. J. Syst. Evol. Microbio. 51, 559–563 (2001)
Brosius, J., et al.: Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J. Mol. Biol. 148, 107–127 (1981)
Ekinci, M., et al.: Effect of plant growth promoting rhizobacteria on growth, nutrient, organic acid, amino acid and hormone content of cauliflower (Brassica oleracea L. var. Botrytis) transplants. Acta Sci. Pol., Hortorum Cultus. 13(6), 71–85 (2014)
Shaukat, K., et al.: Growth responses of Helianthus annuus to plant growth promoting rhizobacteria used as a biofertilizer. J. Agric. Res. 1, 573–581 (2006a)
Shaukat, K., et al.: Growth responses of Triticum aestivum to plant growth promoting rhizobacteria used as a biofertilizer. Res. J. Microbiol. 1, 330–338 (2006b)
Kokalis-Burelle, N., et al.: Amendment of muskmelon and watermelon transplant media with plant growth promoting rhizobacteria: effects on transplant quality, disease, and nematode resistance. HortTech. 13(3), 476–482 (2003)
Gül, A., et al.: Effects of nutrition and Bacillus amyloliquefaciens on tomato (Solanum lycopersicum L.) growing in perlite. Spanish J. Agric. Res. 6(3), 422–429 (2008)
Misra, M., et al.: Efficiency of plant growth promoting rhizobacteria for the enhancement of Cicer arietinum L. growth and germination under salinity. Adv. Biol. Res. 4(2), 92–96 (2010)
Turan, M., et al.: Plant growth–promoting rhizobacteria improved growth, nutrient, and hormone content of cabbage (Brassica oleracea) seedlings. Türkish J. Agric. 38, 327–333 (2014)
Wydra, K., Semrau, J.: Phenotypic and molecular characterization of the interaction of antagonistic bacteria with Ralstonia solanacearum causing tomato bacterial wilt. In: Zeller, W. (ed.) 1st International Symposium on Biological Control of Bacterial Plant Diseases, pp. 112–118. Darmstadt, Germany (2005)
S. Compant, B. Duffy, J. Nowak, C. Clement, E. A. Barka, (2005) Use of Plant Growth-Promoting Bacteria for Biocontrol of Plant Diseases: Principles, Mechanisms of Action, and Future Prospects. Applied and Environmental Microbiology 71 (9):4951–4959
Acknowledgments
This research was funded by Hibah Guru Besar Universitas Andalas, Contract No. 524/XIV/A/UNAND-2016 on May 9, 2016. The authors would like to sincerely thank the Rector of Andalas University.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer International Publishing AG
About this paper
Cite this paper
Habazar, T., Yanti, Y., Reflinaldon, Arneti, Nasution, C.R. (2017). Screening of Rhizobacterial Indigenous as Biocontrol Agents Against Bacterial Wilt on Chili Using In Planta Technique. In: Isnansetyo, A., Nuringtyas, T. (eds) Proceeding of the 1st International Conference on Tropical Agriculture. Springer, Cham. https://doi.org/10.1007/978-3-319-60363-6_28
Download citation
DOI: https://doi.org/10.1007/978-3-319-60363-6_28
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-60362-9
Online ISBN: 978-3-319-60363-6
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)