Abstract
Pratylenchus coffeae is a plant-parasitic nematode, which is a major cause in the decline of coffee plant yield in Indonesia. The loss caused by P. coffeae is up to 78%. This research is one of the efforts being made in biological control of P. coffeae. Isolates were screened by a pathogenicity test in vitro with a spore suspension at a density of 106 spores · mL−1 against P. coffeae nematodes at different life stages (J2, J3, J4 and adult). The pathogenicity test was done by choosing fungal isolates that had ability to cause >50% nematode mortality. P. coffeae nematodes were tested by an in vitro method with a fungal suspension and liquid culture filtrate (crude enzyme). Observations were done to determine the percentages of P. coffeae nematode destruction. Based on the test, isolate PIVB0402A had a very powerful mechanism of mortality in the pathogenicity process against P. coffeae nematodes. This work showed that isolate PIVB0402A caused the highest mortality of up to 100% in P. coffeae.
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The authors thank the Nematology Laboratory, Faculty of Agriculture, Universitas Gadjah Mada, for facilitating this research.
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Aldina, R.F., Indarti, S., Wibowo, A. (2017). Pathogenicity of Nematofagous Fungus for Control of Pratylenchus coffeae Nematodes on Coffee Plants. In: Isnansetyo, A., Nuringtyas, T. (eds) Proceeding of the 1st International Conference on Tropical Agriculture. Springer, Cham. https://doi.org/10.1007/978-3-319-60363-6_23
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DOI: https://doi.org/10.1007/978-3-319-60363-6_23
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