Abstract
Begomovirus is the biggest genus of the Geminiviridae family, which triggers melon crop failure in Indonesia. Begomovirus infection is characterized by the emergence of chlorosis spots within the leaves, as well as curly and dried leaves, yellowish leaf-stalks, and stunted plants. But melon with resistance to Begomovirus genetically has not been found until now. This study was conducted to identify resistance against Begomovirus in melon using a SCAR marker. The melon variety Hikapel was used in this study. A total of 58 melon plants were observed visually to score the Begomovirus symptoms. Begomovirus infection was then detected using CPA5 and CPA2 markers. If Begomovirus particles are amplified at 770 bp, this result is identical to Squash leaf curl Philippine virus (SLCPV). Detection of Begomovirus resistance was conducted in nine samples using a PCR method. The results showed that the virus particle was amplified at 770 bp using CP primers, and melon DNA was amplified at 1198 bp using SCAR. This revealed that Hikapel melon had resistance to SLCPV. There have been no previous reports about melon with genetically proven resistance to Begomovirus.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Arumuganathan, K., Earle, E.D.: Nuclear DNA content of some important plants species. Plant. Mol. Biol. Rep. 9, 208–218 (1991)
Idris, A.M., et al.: Melon chlorotic leafcurl virus: characterization and differential reassortment with closest relatives reveal adaptive virulence in the Squash leaf curl virus clade and host shifting by the host-restricted Bean calico mosaic virus. J. Virol. 82, 2008, 1959–1967
Varma, A., et al.: Emergence of Begomovirus: a major threat to vegetable production in Southeast Asia. SEAVEG2012 Regional Symposium. 24–26 January (2012)
Julijantono, I.: Identification the causes, vector and molecular marker linked to resistance gene against Begomovirus in melon. Dissertation, Agriculture Faculty of Universitas Gadjah Mada (2012)
Sohrab, S.S., et al.: Chlorotic curly stunt: a severe Begomovirus disease of bottle ground in Northern India. Indian J. Virol. 21(1), 56–63 (2010)
Varma, A., Malathi, V.G.: Emerging geminivirus problems: a serious threat to crop production. Ann. Appl. Biol. 142, 145–164 (2003)
Dolores, L.M.: Management of pepper viruses. In: Proceeding of the AVNET II Final Workshop. Tainan. AVRDC. pp. 334–342 (1996)
Sidiq, Y.: Identification and development of molecular markers sequence characterized amplified region adrift resistance gene against melon (Cucumis melo L.). Thesis (Unpublished). Biology Faculty, Universitas Gadjah Mada, Yogyakarta (2014)
Sattar, M.N.: Diversity and Interactions of Begomoviruses and their associated DNA-Satellites. Doctoral thesis. Uppsala. Swedish University of Agricultural Sciences (2012)
Ahmed, M., et al.: Phylogenetic analysis of Bemisia tabaci (Hemiptera: Aleyrodidae) populations from cotton plants in Pakistan, China, and Egypt. J. Pest. Sci. 83(2), 135–141 (2010)
Perring, T.M., et al.: Identification of a whitefly species by genomic and behavioral studies. Science. 259, 74–77 (1993)
Brown, J.K., Idris, A.M.: Genetic differentiation of whitefly Bemisia tabaci mitochondrial cytochrome oxidase I, and phylogeographic concordance with the coat protein of the plant virus genus Begomovirus. Ann. Entomol. Soc. Am. 98(6), 827–837 (2005)
Morin, S., et al.: The GroEL protein of the whitefly Bemisia tabaci interacts with the coat protein of trasmissible and nontransmissible Begomoviruses in the yeast two-hybrid system. Virology. 276, 404–416 (2000)
Daryono, B.S., Qurrahman, T.: Inheritance of resistance to powdery mildew (Podosphaera xanthii (Castag.) Braun et shishkoff) in melon (Cucumis melo L.) Indones J Plant Prot. 15(1), 1–6 (2009)
Wang, Y.H., et al.: Genetic mapping of a fusarium wilt resistance gene (Fom-2) in melon (Cucumis melo L.) Mol. Breed. 6(4), 379–389 (2000)
Pitrat, M., Lecoq, H.: Inheritance of resistance gene to Cucumber mosaic virus transmission by Aphis gossypii in Cucumis melo. Phytopatology. 70, 958–961 (1980)
Daryono, B.S., et al.: Biological characterization and complete nucleotide sequence of coat protein of Kyuri green mottle mosaic virus isolated from angled loofah in Indonesia. J. Agric. Sci. 51(1), 45–52 (2005)
Daryono, B.S., et al.: Inheritance of resistance to Kyuri green mottle mosaic virus in melon. Sabrao J. Breed Genet. 48(1), 33–40 (2016)
Mubin, M., et al.: Characterization of Begomovirus components from a weed suggests that Begomoviruses may associate with multiple distinct DNA satellites. Virus Genes. 40(3), 452–457 (2010)
Thomas, D.M., et al.: Resistance to Tomato yellow leaf curl virus accumulation in the tomato wild relative Solanum habrochaites associated with the C4 viral protein. Mol. Plant-Microbe Interact. 24(7), 849–861 (2011)
Raja, P.: Methylation of Geminivirus genomes: investigating its role as a host defense and evaluating its efficacy as a model to study chromatin methylation in plants. Diss.:Ohio State University (2010)
Obbard, D.J., et al.: The evolution of RNAi as a defence against viruses and transposable elements. Philos. Trans. R. Soc. Lond. Ser. B Biol. Sci. 364(1513), 99–115 (2009)
Lodish, H.F., et al.: Micromanagement of the immune system by microRNAs. Nat. Rev. Immunol. 8(2), 120–130 (2008)
Welker, N.C., et al.: Genes misregulated in C. elegans deficient in Dicer, RDE-4, or RDE-1 are enriched for innate immunity genes. RNA. 13(7), 1090–1102 (2007)
Daryono, B.S., et al.: Development of random amplified polymorphism DNA markers linked to powdery mildew resistance gene in melon. Indones. J. Biotech. 16(2), 76–82 (2011)
Luongo, L., et al.: Development of SCAR markers and PCR assay for Fusarium oxysporum f. sp. melonis race 2-specific detection. JPP. 94(1), 193–199 (2012)
Liao, J.Y., et al.: Identification of Squash leaf curl Philippine virus on Benincasa hipsida in Taiwan. Plant. Pathol. Bull. 16, 11–18 (2007)
Bela-ong, D.B., Bajet, N.B.: Molecular detection of whitefly-transmissible geminiviruses (family Geminiviridae, genus Begomovirus) in the Philippines. Philipp. J. Sci. 136, 87–101 (2007)
Flock, R.A., Mayhew, D.E.: Squash leaf curl, a new disease of cucurbits in California. Plant Dis. 65, 75–76 (1981)
Keane, P.J., Kerr, A.: Factors affecting disease development. In: Brown, J.F., Ogle, H.J. (eds.) Plant Pathogens and Plant Disease. Rockvale Publications, Armidale (1997)
Acknowledgments
The author thanks the Indonesian Ministry of Finance for Riset Inovatif-Produktif (RISPRO) grant no. PRJ-622/LPDP/2016 in 2016 and the Indonesian Ministry of Higher Education and Technology for Program Menuju Doktor Sarjana Unggul grant no. 4543/UN1-P.III/LT/DIT-LIT/2016.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer International Publishing AG
About this paper
Cite this paper
Subiastuti, A.S., Fatmawati, U.E., Daryono, B.S. (2017). Detection of Resistance Against Begomovirus Using a SCAR Marker in Melon (Cucumis melo L. cv. Hikapel). In: Isnansetyo, A., Nuringtyas, T. (eds) Proceeding of the 1st International Conference on Tropical Agriculture. Springer, Cham. https://doi.org/10.1007/978-3-319-60363-6_2
Download citation
DOI: https://doi.org/10.1007/978-3-319-60363-6_2
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-60362-9
Online ISBN: 978-3-319-60363-6
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)