Advertisement

Molecular Performance of the MTG (Moderat Tahan Gano) Oil Palm Variety Based on SSR Markers

  • Lollie Agustina P. Putri
  • Hot Setiado
  • Indra Syahputra
  • Deni Arifiyanto
  • Rika Hardianti
Conference paper

Abstract

The oil palm, an economically important crop tree in Indonesia, has been one of the world’s major sources of edible oil and a significant precursor of biodiesel fuel. The objectives of this study were to know the DNA profiles of oil palm trees from the commercial Moderat Tahan Gano (MTG) variety collections from PT Socfindo. A total of 15 individual palms of the MTG variety were used for analysis. In this experiment, the DNA profile diversity was assessed using three loci of the oil palm’s specific simple sequence repeat (SSR) markers. The results of the experiment indicated that out of three SSR marker loci evaluated, two loci were monomorphic in all genera of palms evaluated, while one was polymorphic. On the other hand, some allele profiles differed from the rest, indicating possibilities of either variants or mislabeled materials. These results demonstrated that the SSR marker can be used to evaluate genetic relatedness among progeny derived from different parents and to detect either genetic variants or mislabeling.

Keywords

Diversity MTG Variety Oil Palm SSR Marker 

Notes

Acknowledgments

The authors gratefully acknowledge the full support received from PT Socfindo Indonesia for the present research and the University of Sumatera, Utara Ministry of Research and Technology and Higher Education, Republic of Indonesia. The support comes under the research grant BP-PTN USU of year 2016 Contract Number 27/UN5.2.3.1/PPM/SP/2016.

References

  1. 1.
    Corley, R.H.V., Thinker, P.: The Oil Palm, 4th edn. Blackwell Science Ltd, London (2003)CrossRefGoogle Scholar
  2. 2.
    Asmono, D., et al.: Pemetaan genom pengendali produktivitas minyak pada kelapa sawit. [Genome mapping controller on palm oil productivity]. Integrated competitive research report (RUT) VII. Indonesian Institute of Science (2000) (in Bahasa Indonesia)Google Scholar
  3. 3.
    Presti, F.T., Wasko, A.P.: A review of microsatellite markers and their application on genetic diversity studies in parrots. Open J. Genet. 402, 69 (2014)CrossRefGoogle Scholar
  4. 4.
    Gupta, P.K., et al.: Microsatellites in plants: a new class of molecular markers. Curr. Sci. 70, 45–54 (1996)Google Scholar
  5. 5.
    Akkaya, M.S., et al.: Length polymorphisms of simple sequence repeat DNA in soybean. Genetics. 132, 1131–1139 (1992)PubMedPubMedCentralGoogle Scholar
  6. 6.
    Gupta, P.K., Varshney, R.K.: The development and use of microsatellite markers for genetic analysis and plant breeding with the emphasis on bread wheat. Euphytica. 113, 163–185 (2000)CrossRefGoogle Scholar
  7. 7.
    Billotte, N., et al.: Development, characterization and across-taxa utility of oil palm (Elaeis guineensis Jacq.) microsatellite markers. Genome. 44, 413–425 (2001)CrossRefPubMedGoogle Scholar
  8. 8.
    Taeprayoon, P., et al.: Genetic background of three commercial oil palm breeding populations in Thailand revealed by SSR markers. Aust. J. Crop. Sci. 9, 281–288 (2015)Google Scholar
  9. 9.
    Singh, R., et al.: Development of simple sequence repeat (SSR) markers for oil palm and their application in genetic mapping and fingerprinting of tissue culture clones. Asia-Pac. J. Mol. Biol. Biotech. 15, 121–131 (2007)Google Scholar
  10. 10.
    Hama-Ali, E.O., et al.: Illegitimacy and sibship assignments in oil palm (Elaeis guineensis Jacq.) half-sib families using single locus DNA microsatellite markers. Mol. Biol. Rep. 42, 917–925 (2015)CrossRefPubMedGoogle Scholar
  11. 11.
    Billotte, N., et al.: QTL detection by multi-parent linkage mapping in oil palm (Elaeis guineensis Jacq.) Theor. Appl. Genet. 120, 1673–1687 (2010)CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Ting, N.C., et al.: Identification of QTLs associated with callogenesis and embryogenesis in oil palm using genetic linkage maps improved with SSR markers. PLoS One. 8, 53076 (2013)CrossRefGoogle Scholar
  13. 13.
    McCouch, S.R., et al.: Development and mapping of 2,240 new SSR markers for rice (Oryza sativa L.) DNA. Research. 9, 257–279 (2002)Google Scholar
  14. 14.
    Oroczo-Castillo, C., et al.: Detection of genetic diversity and selective gene introgression in coffee using RAPD markers. Theor. Appl. Genet. 87, 934–940 (1994)Google Scholar
  15. 15.
    Toruan-Mathius, N., Hutabarat, T.: Pemanfaatan teknik penanda molekuler dalam usaha meningkatkan produktivitas tanaman perkebunan. [Utilization of molecular marker techniques in an effort to increase the productivity of plantation crops]. News Res. Center Biotech Plant. II. 1, 2–9 (1997)Google Scholar
  16. 16.
    Bilotte, N., et al.: Microsatellite-based high density linkage map in oil palm (Elaeis guineensis Jacq.) Theor. Appl. Genet. 110, 754–765 (2005)CrossRefGoogle Scholar
  17. 17.
    Asif, M., et al.: High resolution metaphor agarose gel electrophoresis for genotyping with microsatellite markers. Pak. J. Agric. Sci. 45, 75–79 (2008)Google Scholar

Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • Lollie Agustina P. Putri
    • 1
  • Hot Setiado
    • 1
  • Indra Syahputra
    • 2
  • Deni Arifiyanto
    • 2
  • Rika Hardianti
    • 1
  1. 1.Faculty of Agriculture, Department of AgroecotechnologyUniversity of Sumatera UtaraMedanIndonesia
  2. 2.PT Socfin IndonesiaMedanIndonesia

Personalised recommendations