Abstract
Fusarium spp. have a significant status as a plant pathogen in maize cultivation and post-harvest handling in Indonesia, for they not only devastate the crop in the field and during storage but also have potential capability to be a mycotoxin contaminant in maize-based food or feed. This research aimed to detect mycotoxin-encoding genes from 24 isolates of Fusarium spp. collected from maize kernels harvested in several maize cultivation center areas in Java and one isolate from Lombok Island, using specific primers. These Fusarium spp. had already been molecularly characterized and grouped as four species: F. verticillioides, F. graminearum, F. proliferatum, and F. asiaticum. The results showed that genes encoding fumonisin, deoxynivalenol, and zearalenone were detected in F. verticillioides, and genes encoding deoxynivalenol and zearalenone were detected in F. graminearum and F. asiaticum, while those mycotoxin-encoding genes were not detected in F. proliferatum on the basis of the specific primers used in this research. This is a principal study to considerably contribute further research in observing conditions that will be favorable for expression of those genes, in order to better manage maize post-harvest handling to avoid mycotoxin contamination.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Mostafa, A.T., Kazem, S.S.: Fungi associated with harvested corn grains of Golestan province in Iran. Ann. Biol. Res. 2(5), 681–688 (2011)
Komen, J.J., Mutoko, C.M., Wanyama, J.M., Rono, S.C., Mose, L.O.: Economics of post-harvest maize grain losses in trans Nzoia and Uasin Gishu Districts of Northwest Kenya. In: Proceedings of the 10th KARI biennial scientific conference, Nairobi, Kenya, 12–17 November 2006 (2006)
Ivic, D., et al.: Fusarium spp. contamination of wheat, maize, soybean, and pea in Croatia. ArhHigRadaToksikol. 60(4), 435–442 (2009). doi:10.2478/10004-1254-60-2009-1963
Glenn, A.E.: Mycotoxigenic Fusarium species in animal feed. Anim. Feed Sci. Technol. 137(3–4), 213–240 (2007)
Alkadri, D.: Fusarium species responsible for mycotoxin production in wheat crop: involvement in food safety. PhD thesis, Bologna University (2012)
Bennet, J.W., Klinch, M.: Mycotoxins. Clin. Microbiol. Rev. 16(3), 497–516 (2003)
Proctor, R.H., et al.: A fumonisin biosynthetic gene cluster in Fusarium oxysporum strain O-1890 and the genetic basis for B versus C fumonisin production. Fungal Genet. Biol. 45(6), 1016–1026 (2008). doi:10.1016/j.fgb.2008.02.004
Bush, B.J., et al.: Infection and fumonisin production by Fusarium verticillioides in developing maize kernels. Phytopathology. 94, 88–93 (2004). doi:10.1094/PHYTO.2004.94.1.88
Sobrova, P., et al.: Deoxynivalenol and its toxicity. Interdiscip. Toxicol. 3(3), 94–99 (2010)
Somantri, A.S., Miskiyah.: Sistem kemanan pangan berbahan baku jagung [Food safety system on corn]. Buletin Teknologi Pascapanen Pertanian. 8(2), 112–119. (2012) (in Bahasa Indonesia)
González, H.H., et al.: Natural co-occurrence of fumonisins, deoxynivalenol, zearalenone and aflatoxins in field trial corn in Argentina. Food Addit. Contam. 16(12), 565–569 (1999). doi:10.1080/026520399283704
Molina, M., Gianuzzi, L.: Modelling of aflatoxin production by Aspergillus parasiticus in a solid medium at different temperatures, pH and propionic acid concentrations. Food Res. Int. 35(6), 585–594 (2002). doi:10.1016/S0963-9969(01)00161-2
Sanchis, V., Magan, N.: Environmental profiles for growth and mycotoxin production. In: Manga, N., Olsen, M. (eds.) Mycotoxins in Food: Detection and Control, p. 488. Woodhead Publishing Ltd, Cambridge (2004)
Magan, N., et al.: Limiting mycotoxins in stored wheat. Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess. 27(5), 644–650 (2010). doi:10.1080/19440040903514523
Cowger, C., Arrellano, C.: Plump kernels with high deoxynivalenol linked to late Gibberella zeae infection and marginal disease conditions in winter wheat. Phytopathology. 100(7), 719–728 (2010). doi:10.1094/PHYTO-100-7-0719
Del Ponte, E.M., et al.: Influence of growth stage on Fusarium head blight and deoxynivalenol production in wheat. J. Phytopathol. 155, 577–581 (2007). doi:10.1111/j.1439-0434.2007.01281.x
Acknowledgments
The authors express their deepest gratitude to the Indonesian Toray Science Foundation [through STRG 20, 2014] and the Faculty of Agriculture, Universitas Gadjah Mada, which supported some parts of this research.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer International Publishing AG
About this paper
Cite this paper
Dhanti, K.R., Widiastuti, A., Joko, T. (2017). Detection of Mycotoxin-Encoding Genes in Fusarium spp. Isolated from Maize Kernels in Indonesia. In: Isnansetyo, A., Nuringtyas, T. (eds) Proceeding of the 1st International Conference on Tropical Agriculture. Springer, Cham. https://doi.org/10.1007/978-3-319-60363-6_11
Download citation
DOI: https://doi.org/10.1007/978-3-319-60363-6_11
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-60362-9
Online ISBN: 978-3-319-60363-6
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)