Abstract
Production of wine from grape juice is predominantly the result of complex enzymatic reactions. The primary bioconversion of grape sugar to ethanol and CO2 by the yeast Saccharomyces cerevisiae is catalyzed by cytosolic enzymes. In spontaneous must fermentations, also strains of Saccharomyces bayanus or interspecies hybrids may dominate, probably because of better adaptation to specific environmental conditions. In addition, various enzymes released into the must influence the final composition, color, and sensory properties of wines. These enzymes originate from the grape itself, from epiphytic fungi like Botrytis cinerea, and from yeasts and bacteria associated with vineyards and wine cellars. Especially non-Saccharomyces yeasts, also called “wild” yeasts, belonging to the genera Kloeckera, Candida, Debaryomyces, Rhodotorula, Pichia, Wickerhamomyces, Zygosaccharomyces, Hanseniaspora, Kluyveromyces, and Metschnikowia, secrete different hydrolytic enzymes (esterases, lipases, glycosidases, glucanases, pectinases, amylases, proteases) which interact with grape compounds. Apart from yeasts, enzymes of lactic acid bacteria have a significant impact on wine quality.
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Adams DO (2006) Phenolics and ripening in grape berries. Am J Enol Vitic 57:249–256
Alexandre H, Heintz D, Chassagne D, Guilloux-Benatier M, Charpentier C, Feuillat M (2001) Protease A activity and nitrogen fractions released during alcoholic fermentation and autolysis in enological conditions. J Ind Microbial Biotechnol 26:235–240
Apud GR, Stivala MG, Fernadez PA, Rodriquez Vaquero MJ (2013) Proteolytic activity of Oenococcus oeni enables the increase in antioxidant and antihypertensive activities from wine. Curr Pharm Biotechnol 14:809–813
Barbagallo RN, Spagna G, Palmieri R, Torriani S (2004) Assessment of β-glucosidase activity in selected wild strains of Oenococcus oeni for malolactic fermentation. Enzym Microb Technol 34:292–296
Bäumlisberger M, Moellecken U, König H, Claus H (2015) The potential of the yeast Debaryomyces hansenii H525 to degrade biogenic amines in food. Microorganisms 3:839–850
Bedrinana RP, Queipo AL, Valles B (2012) Screening of enzymatic activities in non-Saccharomyces cider yeasts. J Food Biochem 36:683–689
Belda I, Ruiz J, Alastruey-Izquierdo A, Nacascues E, Marquina D, Santos A (2016a) Unraveling the enzymatic basis of wine “flavorome”: a phylo-functional study of wine related yeast species. Front Microbiol. doi:10.3389/fmicb.2016.00012
Belda I, Conchillo LB, Ruiz J, Navacués E, Marquina D, Santos A (2016b) Selection and use of pectinolytic yeasts for improving clarification and phenolic extraction in winemaking. Int J Food Microbiol 223:1–8
Benucci I, Esti M, Liburdi K (2014) Effect of free and immobilised stem bromelain on protein haze in white wine. Aust J Grape Wine Res 20:347–352
Benucci I, Esti M, Liburdi K (2015) Effect of wine inhibitors on the proteolytic activity of papain from Carica papaya L. Latex. Biotechnol Prog 31:48–53
Blättel V, Larisika M, Pfeiffer P, Nowak C, Eich A, Eckelt J, König H (2011) β-1-3-Glucanase from Delftia tsuruhatensis strain MV01 and its potential application in vinifivation. Appl Environ Microbiol 77:983–990
Boido E, Lloret A, Medina K, Carreau F, Dellacassa E (2002) Effect of beta-glycosidase activity of Oenococcus oeni on the glycosylated flavor precursors of Tannat wine during malolactic fermentation. J Agric Food Chem 50:2344–2349
Caggianello G, Kleerebezem M, Spano G (2016) Exopolysaccharides produced by lactic acid bacteria: from health-promoting benefits to stress tolerance mechanisms. Appl Microbiol Biotechnol 100:3877–3886
Callejón S, Sendra R, Ferrer S, Pardo I (2014) Identification of a novel enzymatic activity from lactic acid bacteria able to degrade biogenic amines in wine. Appl Microbiol Biotechnol 98:185–198
Callejón S, Sendra R, Ferrer S, Pardo I (2016) Cloning and characterization of a new laccase from Lactobacillus plantarum J16 CECT 8944 catalyzing biogenic amine degradation. Appl Microbiol Biotechnol 100:3113–3312
Canas PMI, Garcia-Romero E, Manso JMH, Fernandez-Gonzalez M (2014) Influence of sequential inoculation of Wickerhamomyces anomalus and Saccharomyces cerevisiae in the quality of red wines. Eur Food Res Technol 239:27–28
Charoenchai C, Fleet GH, Henschke PA, Todd BEN (1997) Screening of non-Saccharomyces wine yeasts for the presence of extracellular hydrolytic enzymes. Aust J Grape Wine Res 3:2–8
Chassagne DS, Vernizeau M, Nedjma M, Alexandre H (2005) Hydrolysis and sorption by Saccharomyces cerevisiae strains of Chardonnay grape must glycosides during fermentation. Enzym Microb Technol 37:212–217
Chasseriaud L, Miot-Sertier C, Coulon J, Iturmendi N, Moine V, Albertin W, Bely M (2015) A new method for monitoring the extracellular proteolytic activity of wine yeasts during alcoholic fermentation of grape must. J Microbiol Methods 119:176–179
Christ E, Kowalczyk M, Zuchowska M, Claus H, Löwenstein R, Szopinska-Morawska A, Renaut J, König H (2015) An exemplary model study for overcoming stuck fermentation during spontaneous fermentation with the aid of a Saccharomyces triple hybrid. J Agric Sci 7:18–34
Claus H (2007) Extracellular enzymes and peptides of lactic acid bacteria: significance for vinification. Deutsche Lebensmittel-Rundschau 11:505–551
Claus H, Filip Z (1988) Behaviour of phenoloxidases in the presence of clays and other soil-related adsorbents. Appl Microbiol Biotechnol 28:506–511
Claus H, Tenzer S, Sobe M, Schlander M, König H, Fröhlich J (2014) Effect of carboxymethyl cellulose on tartrate salt, protein and colour stability of red wine. Aust J Wine Grape Res 20:186–193
Comitini F, Gobbi M, Domizio P, Romani C, Lencioni L, Mannazzu I, Ciani M (2011) Selected non-Saccharomyces wine yeasts in controlled multistarter fermentations with Saccharomyces cerevisiae. Food Microbiol 28:873–882
Costello PJ, Siebert TE, Solomon MR, Bartowsky EJ (2013) Synthesis of fruity ethyl esters by acyl coenzyme A: alcohol acyltransferase activities in Oenococcus oeni and Lactobacillus plantarum. J Appl Microbiol 114:797–806
D’Incecco N, Bartowsky EJ, Kassara S, Lante A, Spettoli P, Henschke PA (2004) Release of glycosidically bound flavour compounds from Chardonnay by Oenococcus oeni during malolactic fermentaion. Food Microbiol 21:257–265
Daenen L, Saison D, Sterckx F, Delvaux FR, Verachtert H, Derdelelinckx G (2008) Screening and evaluation of the glucoside hydrolase activity in Saccharomyces and Brettanomyces brewing yeasts. Appl Microbiol 104:478–488
Davis CR, Wibowo D, Fleet GH, Lee TH (1988) Properties of wine lactic acid bacteria: their potential enological significance. Am J Enol Vitic 39:137–142
Deckwart M, Carstens C, Webber-Witt M, Schaefer V, Eichhorn L, Schroter F, Fischer M, Brockow K, Christmann M, Paschke-Kratzin A (2014) Impact of wine manufacturing practice on the occurrence of fining agents with allergenic potential. Food Add Contamin Part A – Chem Anal Contr Expo Risk Assess 31:1805–1817
Delaquis P, Cliff M, King B, Girard B, Hall J, Reynolds A (2000) Effect of two commercial malolactic cultures on the chemical and sensory properties of Chancellor wines with different yeasts and fermentation temperatures. Am J Enol Vitic 51:42–48
Delcroix A, Gunata Z, Sapis LC, Salmon JM, Baynone C (1994) Glycosidase activities of three enological yeast strains during winemaking: effect on the terpenol content of Muscat wine. Am J Enol Vitic 45:291–296
Dubourdieu D, Ribéreau-Gayon P, Fournet B (1981) Structure of the extracellular β-D-glucan from Botrytis cinerea. Carbohydr Res 93:294–229
El Rayess Y (2014) Wine: phenolic composition, classification and health benefits. Nova, New York. ISBN: 978-1-63321-048-6
Enrique M, Ibanez A, Marcos JF, Yuste M, Martinez M, Valles S, Manzanares P (2010) Beta-Glucanases as a tool for the control of wine spoilage yeasts. J Food Sci 75:M41–M45
Eschstruth A, Divol B (2011) Comparative characterization of endo-polygalacturonase (Pgu1) from Saccharomyces cerevisiae and Saccharomyces paradoxus under winemaking conditions. Appl Microbiol Biotechnol 91:623–634
Esteban-Torres M, Barcenilla JM, Mancheno JM, de las Rivas B, Munoz R (2014) Characterization of a versatile arylesterase from Lactobacillus plantarum active on wine esters. J Agric Food Chem 62:5118–5125
Esti M, Benucci I, Lombardelli C, Liburdi K, Garzillo AMV (2013) Papain from papaya (Carica papaya L.) fruit and latex: preliminary characterization in alcoholic-acidic buffer for wine application. Food Bioprod Process 91:595–598
Farias ME, de Nadra MCM (2000) Purification and partial characterization of Oenococcus oeni exoprotease. FEMS Microbiol Lett 185:263–266
Fernández M, Úbeda JF, Briones AI (2000) Typing of non-Saccharomyces yeasts with enzymatic activities of interest in wine-making. Int J Food Microbiol 59:29–36
Ferreira AM, Climaco MC, Faia AM (2001) The role of non-Saccharomyces species in releasing glycosidic bound fraction of grape aroma components – a preliminary study. J Appl Microbiol 91:67–71
Folio P, Ritt JF, Alexandre H, Remize F (2008) Characterization of EprA, a major extracellular protein of Oenococcus oeni with protease activity. Int J Food Microbiol 127:26–31
Fragopoulou E, Antonopoulou S, Demopoulos CA (2002) Biologically active lipids with antiatherogenic properties from white wine and must. J Agric Food Chem 50:2684–2694
Friel D, Gomez Pessoa N, Vandenbol M, Jijakli MH (2007) Separate and combined disruptions of two exo-β-1,3-glucanase genes decrease the efficiency of Pichia anomala (strain K) biocontrol against Botrytis cinerea on apples. Mol Plant-Microbe Interact 20:371–379
Fugelsang KC, Edwards CG (2007) Wine microbiology: practical applications and procedures, 2nd edn. Springer, Berlin
Gallander JF, Peng AC (1980) Lipid and fatty acid composition of different wine grapes. Am J Enol Vitic 31:24–27
Gil JV, Manzanares P, Genoves S, Valles S, Gonzalez-Candelas L (2005) Over-production of the major exoglucanase of Saccharomyces cerevisiae leads to an increase in the aroma of wine. Int J Food Microbiol 103:57–68
Girbau T, Stummer BE, Pocock KF, Baldock GA, Scott ES, Waters EJ (2004) The effect of Uncinula necator (powdery mildew) and Botrytis cinerea infection of grapes on the levels of haze-forming pathogenesis-related proteins in grape juice and wine. Aust J Grape Wine Res 10:125–133
Gonzales-Pombo O, Farina L, Carreau F, Batista-Viera F, Brena BM (2011) A novel extracellular beta-glucosidase from Issatchenkia terricola: immobilization and application for aroma enhancement of white Muscat wine. Process Biochem 46:385–389
Gravesse C, Lepoivre P, Jijakli MH (2003) Characterization of the exoglucanase-encoding gene PaEXG2 and study of its role in the biocontrol activity of Pichia anomala strain K. Phytopathology 93:1145–1152
Grimaldi A, McLean H, Jiranik V (2000) Identification and partial characterization of glycosidic activities of commercial strains of the lactic acid bacterium Oenococcus oeni. Am J Enol Vitic 51:362–369
Grimaldi A, Bartowsky R, Jiranik V (2005a) A survey of glycosidase activities of commercial wine strains of Oenococcus oeni. Int J Food Microbiol 105:233–244
Grimaldi A, Bartowsky E, Jiranik V (2005b) Screening of Lactobacillus spp. and Pediococcus spp. for glycosidase activities that are important in oenology. J Appl Microbiol 99:1061–1069
Guilloux-Benatier M, Pageault O, Feuillat M (2000) Lysis of yeast cells by Oenococcus enzymes. J Ind Microbiol Biotechnol 25:193–197
Hjelmeland AK, Ebeler SA (2015) Glycosidically bound volatile aroma compounds in grapes and wine: a review. Am J Enol Vitic 66:1–11
Hu K, Zhu XL, Mu H, Ma Y, Ullah N, Tao YS (2016) A novel extracellular glycosidase activity from Rhodotorula mucilaginosa: its application potential in wine aroma enhancement. Lett Appl Microbiol 62:169–176
Iranzo JFU, Pérez AIB, Cañas PMI (1998) Study of oenological characteristics and enzymatic activities of wine yeasts. Food Microbiol 15:399–406
Izzo R, Muratore G (1993) Seed lipids from some varieties of grapes grown in Sicily: note 1. Fatty acid composition. Riv Ital Sostanze Grasse 70:601–604
Jaeckels N, Tenzer S, Rosch A, Scholten G, Decker H, Fronk P (2015) β-Glucosidase removal due to bentonite fining during winemaking. Eur Food Res Technol 241:253–262
Jimenez N, Esteban-Torres M, Miguel Mancheno J, de las Rivas B, Munoz R (2014) Tannin degradation by a novel tannase enzyme present in some Lactobacillus plantarum strains. Appl Environ Microbiol 80:2991–2997
Jolly NP, Varela C, Pretorius IS (2014) Not your ordinary yeast: non-Saccharomyces yeasts in wine production uncovered. FEMS Yeast Res 14:215–237
Lagace LS, Bisson LF (1990) Survey of yeast proteases for effectiveness of wine haze reduction. Am J Enol Vitic 41:147–155
Lambrechts MG, Pretorius IS (2000) Yeast and its importance to wine aroma: a review. S Afr J Enol Vitic 21:97–129
Liburdi K, Benucci I, Esti M (2014) Lysozyme in wine: an overview of current and future applications. Compr Rev Food Sci Food Saf 13:1062–1073
Llaubéres RM, Richard B, Lonvaud A, Dubourdieau D, Fournet B (1990) Structure of extracellular β-D-glucan from Pediococcus sp., a wine lactic bacterium. Carbohydr Res 203:103–107
López MC, Mateo JJ, Maicas S (2015) Screening of β-glucosidase and β-xylosidase activities in four non-Saccharomyces yeast isolates. J Food Sci 80:C1696–C1704
López MC, Mateo JJ, Maicas S (2016) Screening of Hanseniaspora strains for production of enzymes with potential interest for winemaking. Fermentation 2:1. doi:10.3390/fermentation2010001
Madrigal T, Maicas S, Tolosa JJM (2013) Glucose and ethanol tolerant enzymes produced by Pichia (Wickerhamomyces) isolates from enological ecosystems. Am J Enol Vitic 64:126–133
Maicas S, Mateo JJ (2016) Microbial glycosidases for wine production. Beverages 2:20. doi:10.3390/beverages2030020
Maicas S, Gil JV, Pardo I, Ferrer S (1999) Improvement of volatile composition of wines by controlled addition of malolactic bacteria. Food Res Int 32:491–496
Manca de Nadra MC, Strasser de Saad AM (1995) Polysaccharide production by Pediococcus pentosaceus from wine. Int J Food Microbiol 27:101–106
Manca de Nadra MC, Farίas NE, Moreno-Arribas V, Pueyo E, Polo MC (1997) Proteolytic activity of Leuconostoc oneos: effect on proteins and polypeptides from white wine. FEMS Microbiol Lett 150:135–139
Manca de Nadra MC, Farίas NE, Moreno-Arribas V, Pueyo E, Polo MC (1999) A proteolytic effect of Oenococcus oeni on the nitrogenous macromolecular fraction of red wine. FEMS Microbiol Lett 174:491–496
Mansfield AK, Zoecklein BW, Whitton RS (2002) Quantification of glycosidase activity in selected strains of Brettanomyces bruxellensis and Oenococcus oeni. Am J Enol Vitic 53:303–307
Marangon M, Van Sluyter SC, Robinson EMC, Muhlack RA, Holt HE, Haynes PA, Godden PW, Smith PA, Waters EJ (2012) Degradation of white wine haze proteins by Aspergillopepsin I and II during juice flash pasteurization. Food Chem 135:1157–1165
Marchal R, Berthier L, Legendre L, Marchal-Delahaut L, Jeandet P, Maujean A (1998) Effects of Botrytis cinerea on the must protein electrophoretic characteristics. J Agric Food Chem 46:4945–4949
Marchal R, Warchol M, Cilindre C, Jeandet P (2006) Evidence for protein degradation by Botrytis cinerea and relationships with alteration of synthetic wine properties. J Agric Food Chem 54:5157–5165
Mateo JJ, DiStefano R (1997) Description of the beta-glucosidase activity of wine yeasts. Food Microbiol 14:583–591
Mateo JJ, Maicas S, Thießen C (2015) Biotechnological characterisation of extracellular proteases produced by enological Hanseniaspora isolates. Int J Food Sci Technol 50:218–225
Matthews A, Grimaldi A, Walker M, Bartowsky E, Grbin P, Jiranek V (2004) Lactic acid bacteria as a potential source of enzymes for use in vinification. Appl Environ Microbiol 70:5715–5731
Matthews A, Grbin PR, Jiranek V (2006) A survey of lactic acid bacteria for enzymes of interest to oenology. Aust J Grape Wine Res 12:235–244
Matthews A, Grbin PR, Jiranek V (2007) Biochemical characterisation of the esterase activities of wine lactic bacteria. Appl Microbiol Biotechnol 77:329–337
Maturano YP, Assaf LAR, Toro ME, Nally MC, Vallejo M, Castellanos de Figueroa LI, Combina M, Vazquez F (2012) Multi-enzyme production by pure and mixed cultures of Saccharomyces and non-Saccharomyces yeasts during wine fermentation. J Food Microbiol 155:43–50
Maturano YP, Assof M, Fabani MP, Nally MC, Jofré V, Assaf LAR, Toro ME, Castellanos de Figueroa LI, Vazquez F (2015) Enzymatic activities produced by mixed Saccharomyces and non-Saccharomyces cultures: relationship with wine volatile composition. Antonie Van Leeuwenhoek 108:1239–1256
Merín MG, de Ambrosini VIM (2015) Highly cold-active pectinases under wine-like conditions from non-Saccharomyces yeasts for enzymatic production during winemaking. Lett Appl Microbiol 60:467–474
Merín MG, Mendoza LM, Farías ME, de Ambrosini VIM (2011) Isolation and selection of yeast from wine grape ecosystem secreting cold-active pectinolytic activity. Int J Food Microbiol 147:144–148
Michlmayer H, Nauer S, Brandes W, Schumann C, Kulbe KD, del Hierro AM, Eder R (2012) Release of wine monoterpenes from natural precursors by glycosidases from Oenococcus oeni. Food Chem 135:80–87
Mojsov KD, Andronikov D, Janevski A, Jordeva S, Zezova S (2015) Enzymes and wine – the enhanced quality and yield. Adv Technol 4:94–100
Molnárova J, Vadkertiová R, Stratilová E (2014) Extracellular enzymatic activities and physiological profiles of yeasts colonizing fruit trees. J Basic Microbiol 51:S74–S84
Mostert TT, Divol B (2014) Investigating the proteins released by yeasts in synthetic wine fermentations. Int J Food Microbiol 171:108–118
Mtshali PS, Divol B, van Rensburg P, Du Toit M (2010) Genetic screening of wine-relevant enzymes in Lactobacillus species isolated from South Africa. J Appl Microbiol 108:1389–1397
Muccilli S, Restuccia C (2015) Bioprotective role of yeasts. Microorganisms 3:588–611
Padilla B, Gil JV, Manzanares P (2016) Past and future of non-Saccharomyces yeasts: from spoilage microorganisms to biotechnological tools for improving wine complexity. Front Microbiol. doi:10.3389/fmicrb.2016.00411
Pérez G, Fariña L, Barquet M, Boido E, Gaggero C, Dellacassa E, Carrau F (2011) A quick screening method to identify β-glucosidase activity in native wine yeast strains: application of esculin glycerol agar (EGA) medium. World J Microbiol Biotechnol 27:47–55
Perez-Martin F, Sesena S, Miguel Izquierdo P, Martin R, Llanos Palop M (2012) Screening for glycosidase activities of lactic acid bacteria as a biotechnological tool in oenology. World J Microbiol Biotechnol 28:1423–1432
Perez-Martin F, Sesena S, Miguel Izquierdo P, Llanos Palop M (2013) Esterase activity of lactic acid bacteria isolated from malolactic fermentation of red wines. Int J Food Microbiol 163:153–158
Pfeiffer P, Schlander M, König H (2007) Detection and production of pyroglutamic acid ethyl ester in wine. Deutsche Lebensmittel-Rundschau 103:8–10
Pueyo E, Martinez-Rodriguez A, Polo MC, Santa-Maria G, Bartomé B (2000) Release of lipids during yeast autolysis in model wine. J Agric Food Chem 48:116–122
Quatrini P, Marineo S, Puglia AM (2006) The beta-glucosidase encoding gene from yeast strains isolated from Sicilian musts and wines (DQ010949). Center for Biotechnology Information (NCBI), Bethesda. http://www.ncbi.nlm.nih.gov/. Accessed 15 Oct 2006
Ramirez HL, Gómez Brizuela L, Úbeda Iranzo J, Arevalo-Villena M, Briones Pérez AI (2016) Pectinase immobilization on a chitosan-coated chitin support. J Food Process Eng 39:97–104
Rapp A, Mandery H (1986) Wine aroma. Experientia 42:873–884
Reid VJ, Theron LW, du Toit M, Divol B (2012) Identification and partial characterization of extracellular aspartic protease genes from Metschnikowia pulcherrima IWBT Y1123 and Candida apicola IWBT Y1384. Appl Environ Microbiol 78:6838–6849
Rizzi C, Mainente F, Pasini G, Simonato B (2016) Hidden exogenous proteins in wine: problems, methods of detection and related legislation – a review. Czech J Food Sci 34:93–104
Rodriguez ME, Lopes CA, Broock M, Valles S, Ramon D, Caballero AC (2004) Screening and typing of Patagonian wine yeasts for glycosidase activities. J Appl Microbiol 96:84–95
Rollán GC, Farías ME, Manca de Nadra MC (1993) Protease production by Leuconostoc oenos strains isolated from wine. World J Microbiol Biotechnol 9:587–589
Rollán GC, Farías ME, Manca de Nadra MC (1995) Characterization of two extracellular proteases from Leuconostoc oenos. World J Microbiol Technol 11:153–155
Rollán GC, Farías ME, Strasser de Sand AM, Manca de Nadra MC (1998) Exoprotease activity of Leuconostoc oenos in stress condition. J Appl Microbiol 85:219–233
Rollero S, Mouret JR, Sanchez I, Camarasa C, Ortiz-Julien A, Sablayrolles JM, Dequin S (2016) Key role of lipid management in nitrogen and aroma metabolism in an evolved wine yeast strain. Microb Cell Fact 15. doi:10.1186/s12934-016-0434-6
Rosi L, Costamagna L, Bertuccioli M (1987) Screening for extracellular acid protease(s) production by wine yeasts. J Inst Brew 93:322–324
Rosi I, Vinella M, Domizio P (1994) Characterization of beta-glucosidase activity in yeasts of oenological origin. J Appl Bacteriol 77:519–527
Sabel A, Martens S, Petri A, König H, Claus H (2014) Wickerhamomyces anomalus AS1: a new strain with potential to improve wine aroma. Ann Microbiol 64:483–491
Saerens SMG, Delvaux F, Verstrepen KJ, Van Dijck P, Thevelein JM, Delvaux FR (2008) Parameters affecting ethyl ester production by Saccharomyces cerevisiae during fermentation. Appl Environ Microbiol 74:454–461
Sahay S, Hamid B, Singh P, Ranjan K, Chauhan D, Rana RS, Chaurse VK (2013) Evaluation of pectinolytic activities for oenological uses from psychrotrophic yeasts. Lett Appl Microbiol 57:115–121
Salazar O, Asenjo JA (2007) Enzymatic lysis of microbial cells. Biotechnol Lett 29:985–994
Sarry JE, Günata Z (2004) Plant and microbial glycoside hydrolases: volatile release from glycosidic aroma precursors. Food Chem 87:509–521
Schlander M, Distler U, Tenzer S, Thines E, Claus H (2017) Purification and properties of yeast proteases secreted by Wickerhamomyces anomalus 227 and Metschnikowia pulcherrima 446 during growth in a white grape juice. Fermentation 3:2. doi:10.3390/fermentation3010002
Schmidt S, Rainieri S, Witte S, Matern U, Martens S (2011) Identification of a Saccharomyces cerevisiae glucosidase that hydrolyzes flavonoid glycosides. Appl Environ Microbiol 77:1751–1757
Schwentke J, Sabel A, Petri A, König H, Claus H (2014) The wine yeast Wickerhamomyces anomalus AS1 secretes a multifunctional exo-β-1,3 glucanase with implications for winemaking. Yeast 31:349–359
Sebastian P, Herr P, Fischer U, König H (2011) Molecular identification of lactic acid bacteria occurring in must and wine. South Afr J Enol Vitic 32:300–309
Selitrennikoff CP (2001) Antifungal proteins. Appl Environ Microbiol 67:2883–2894
Spagna G, Barbagallo RN, Palmeri R, Restuccia C, Giudici P (2002) Properties of endogenous beta-glucosidase of a Saccharomyces cerevisiae strain isolated from Sicilian musts and wines. Enzym Microb Technol 31:1030–1035
Strauss MLA, Jolly NP, Lambrechts MG, van Rensburg P (2001) Screening for the production of extracellular hydrolytic enzymes by non-Saccharomyces wine yeasts. J Appl Microbiol 91:182–190
Styger G, Prior B, Bauer FF (2011) Wine flavor and aroma. J Ind Microbiol 38:1145–1159
Sumby KM, Grbin PR, Jiranek V (2010) Microbial modulation of aromatic esters in wine: current knowledge and future prospects. Food Chem 121:1–16
Sumby KM, Jiranek V, Grbin PR (2013) Ester synthesis and hydrolysis in an aqueous environment, and strain specific changes during malolactic fermentation in wine with Oenococcus oeni. Food Chem 141:1673–1680
Szopinska A, Christ E, Planchon S, König H, Evers D, Renaut J (2016) Stuck at work? Quantitative proteomics of environment wine yeast strains reveals the natural mechanism of overcoming stuck. Proteomics 16:593–608
Terrier N, Ponced-Legrand C, Cheynier V (2010) Flavanols, flavonols and dihydroflavonols. In: Morena-Arribas MV, Polo MV (eds) Wine chemistry and biochemistry. Springer, New York, pp 463–507
Theron LW, Divol B (2014) Microbial aspartic proteases: current and potential applications in industry. Appl Microbiol Biotechnol 98:8853–8863
Thilakarathna SH, Rupasinghe HPV (2013) Flavonoid bioavailability and attempts for bioavailability enhancement. Nutrients 5:3367–3387
Torresi S, Fragipane MT, Garzillo AMV, Massantini R, Contini M (2014) Effects of a β-glucanase enzymatic preparation on yeast lysis during aging of sparkling wines. Food Res Int 55:83–92
Ugliano M (2010) Enzymes in winemaking. In: Morena-Arribas MV, Polo MC (eds) Wine chemistry and biochemistry. Springer, New York, pp 103–126
Ugliano M, Henschke PA (2010) Yeasts and wine flavour. In: Morena-Arribas MV, Polo MV (eds) Wine chemistry and biochemistry. Springer, New York, pp 313–392
Ugliano M, Bartowsky EJ, McCarthy J, Moio L, Henschke PA (2006) Hydrolysis and transformation of grape glycosidically bound volatile compounds during fermentation with three Saccharomyces yeast strains. J Agric Food Chem 54:6322–6331
Vaillant H, Formisyn P (1996) Purification of the malolactic enzyme from a Leuconostoc oenos strain and use in a membrane reactor for achieving the malolactic fermentation in wine. Biotechnol Appl Biochem 24:217–223
Vakhlu J, Kour A (2006) Yeast lipases: enzyme purification, biochemical properties and gene cloning. Electron J Biotechnol 9. doi:10.2225/vol9-issue1-fulltext-9
Van Rensburg P, Pretorius IS (2000) Enzymes in winemaking: harnessing natural catalysts for efficient biotransformation – a review. S Afr J Enol Vitic 21:52–73
Van Sluyter SC, Warnock NL, Schmidt S, Anderson P, van Kan JAL, Bacic A, Waters EJ (2013) Aspartic acid protease from Botrytis cinerea removes haze-forming proteins during white wine-making. J Agric Food Chem 61:9705–9711
Van Sluyter SC, McRae JM, Falconer RJ, Smith PA, Bacic A, Waters EJ, Marangon M (2015) Wine protein haze: mechanisms of formation and advances in prevention. J Agric Food Chem 63:4020–4030
Van Wyk H, Divol B (2010) Recovery of endo-polygalacturonase activity in wine yeast and its effect of wine aroma. FEMS Yeast Res 10:58–71
Vaquero I, Macrobal Á, Muñoz R (2004) Tannase activity by lactic bacteria isolated from grape must and wine. Int J Food Microbiol 96:199–204
Velasco SE, Yebra MJ, Monedero V, Ibarburu I, Dueñas MT, Irastorza A (2007) Influence of the carbohydrate source on β-glucan production and enzyme activities involved in sugar metabolism in Pediococcus parvulus 2.6. Int J Food Microbiol 115:325–334
Veza S, Rodríguez-Perez R, Carretero P, Juste S, Caballero ML (2015) Occupational allergic bronchial asthma induced by Lallzyme EX-V, an enzymatic blend sourced from Aspergillus niger used as additive in the wine industry. Occup Environ Med 72:237–223
Villetaz JC, Steiner D, Trogus H (1984) The use of β-glucanase as an enzyme in wine clarification and filtration. Am J Enol Vitic 35:253–256
Vivas NA, Lonvaud-Funel A, Glories Y (1997) Effect of phenolic acids and anthocyanins on growth, viability and malolactic activity of a lactic bacterium. Food Microbiol 14:291–300
Wang Y, Zhang C, Xu Y, Li J (2015) Evaluating potential applications of indigenous yeasts and their β-glucosidases. J Inst Brew 121:642–648
Wibowo D, Eschenbruch R, Davis CR, Fleet GH, Lee TH (1985) Occurrence and growth of lactic bacteria in wine. Am J Enol Vitic 36:302–313
Wigand P, Tenzer S, Schild H, Decker H (2009) Analysis of protein composition of red wine in comparison with rosé and white wines by electrophoresis and high-pressure liquid chromatography (HPLC-MS). J Agric Food Chem 57:4328–4333
Winterhalter P, Skouroumounis GK (1997) Glycoconjugated aroma compounds: occurrence, role and biotechnological transformation. Adv Biochem Eng Biotechnol 55:74–99
Yanai T, Sato N (1999) Isolation and properties of β-glucosidase produced by Debaryomyces hansenii and its application in winemaking. Am J Enol Vitic 50:231–235
Yao J, Guo GS, Ren GH, Liu YH (2014) Production, characterization and applications of tannase. J Mol Catal B Enzym 101:137–147
Younes B, Cilindre C, Villaume S, Parmentier M, Jeandet P, Vasserot Y (2011) Evidence for an extracellular and proteolytic activity secreted by living cells of Saccharomyces cerevisiae PIR1: impact on grape proteins. J Agric Food Chem 59:6239–6246
Younes B, Cilindre C, Jeandet P, Vasserot Y (2013) Enzymatic hydrolysis of thermos-sensitive grape proteins by a yeast protease as revealed by a proteomic approach. Food Res Int 54:1298–1301
Younis OS, Stewart GG (1998) Sugar uptake and subsequent ester and higher alcohol production by Saccharomyces cerevisiae. J Inst Brew 104:255–264
Zeemann WJ, Snyman P, van Wyk SJ (1982) The influence of yeast strain and malolactic fermentation on some volatile bouquet substances and on quality of table wines. In: Proceedings of University of California, Davis, Grape and Wine Centennial Symposium, 1980, pp 242–271
Zinnai A, Venturi F, Quartacci MF, Andreotti A, Andrich G (2010) The kinetic effect of some wine components on the enzymatic hydrolysis of β-glucan. S Afr J Enol Vitic 31:9–13
Zivkovic K, König H, Claus H (2011) Wirkung von Bentonit auf die Laccase-Aktivität in Most und Wein. Deutsche Lebensmittel Rundschau 107:575–582
Zoecklein B, Marcy J, Williams S, Jasinski Y (1997) Effect of native yeasts and selected strains of Saccharomyces cerevisiae on glycosyl glucose, potential volatile terpenes, and selected aglycons of white Riesling (Vitis vinifera L.) wines. J Food Compos Anal 10:55–65
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Claus, H. (2017). Microbial Enzymes: Relevance for Winemaking. In: König, H., Unden, G., Fröhlich, J. (eds) Biology of Microorganisms on Grapes, in Must and in Wine. Springer, Cham. https://doi.org/10.1007/978-3-319-60021-5_13
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