Skip to main content

Microbial Enzymes: Relevance for Winemaking

  • Chapter
  • First Online:

Abstract

Production of wine from grape juice is predominantly the result of complex enzymatic reactions. The primary bioconversion of grape sugar to ethanol and CO2 by the yeast Saccharomyces cerevisiae is catalyzed by cytosolic enzymes. In spontaneous must fermentations, also strains of Saccharomyces bayanus or interspecies hybrids may dominate, probably because of better adaptation to specific environmental conditions. In addition, various enzymes released into the must influence the final composition, color, and sensory properties of wines. These enzymes originate from the grape itself, from epiphytic fungi like Botrytis cinerea, and from yeasts and bacteria associated with vineyards and wine cellars. Especially non-Saccharomyces yeasts, also called “wild” yeasts, belonging to the genera Kloeckera, Candida, Debaryomyces, Rhodotorula, Pichia, Wickerhamomyces, Zygosaccharomyces, Hanseniaspora, Kluyveromyces, and Metschnikowia, secrete different hydrolytic enzymes (esterases, lipases, glycosidases, glucanases, pectinases, amylases, proteases) which interact with grape compounds. Apart from yeasts, enzymes of lactic acid bacteria have a significant impact on wine quality.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   229.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   299.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   299.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  • Adams DO (2006) Phenolics and ripening in grape berries. Am J Enol Vitic 57:249–256

    CAS  Google Scholar 

  • Alexandre H, Heintz D, Chassagne D, Guilloux-Benatier M, Charpentier C, Feuillat M (2001) Protease A activity and nitrogen fractions released during alcoholic fermentation and autolysis in enological conditions. J Ind Microbial Biotechnol 26:235–240

    Article  CAS  Google Scholar 

  • Apud GR, Stivala MG, Fernadez PA, Rodriquez Vaquero MJ (2013) Proteolytic activity of Oenococcus oeni enables the increase in antioxidant and antihypertensive activities from wine. Curr Pharm Biotechnol 14:809–813

    Article  CAS  PubMed  Google Scholar 

  • Barbagallo RN, Spagna G, Palmieri R, Torriani S (2004) Assessment of β-glucosidase activity in selected wild strains of Oenococcus oeni for malolactic fermentation. Enzym Microb Technol 34:292–296

    Article  CAS  Google Scholar 

  • Bäumlisberger M, Moellecken U, König H, Claus H (2015) The potential of the yeast Debaryomyces hansenii H525 to degrade biogenic amines in food. Microorganisms 3:839–850

    Article  PubMed  PubMed Central  Google Scholar 

  • Bedrinana RP, Queipo AL, Valles B (2012) Screening of enzymatic activities in non-Saccharomyces cider yeasts. J Food Biochem 36:683–689

    Article  CAS  Google Scholar 

  • Belda I, Ruiz J, Alastruey-Izquierdo A, Nacascues E, Marquina D, Santos A (2016a) Unraveling the enzymatic basis of wine “flavorome”: a phylo-functional study of wine related yeast species. Front Microbiol. doi:10.3389/fmicb.2016.00012

  • Belda I, Conchillo LB, Ruiz J, Navacués E, Marquina D, Santos A (2016b) Selection and use of pectinolytic yeasts for improving clarification and phenolic extraction in winemaking. Int J Food Microbiol 223:1–8

    Google Scholar 

  • Benucci I, Esti M, Liburdi K (2014) Effect of free and immobilised stem bromelain on protein haze in white wine. Aust J Grape Wine Res 20:347–352

    Article  CAS  Google Scholar 

  • Benucci I, Esti M, Liburdi K (2015) Effect of wine inhibitors on the proteolytic activity of papain from Carica papaya L. Latex. Biotechnol Prog 31:48–53

    Article  CAS  PubMed  Google Scholar 

  • Blättel V, Larisika M, Pfeiffer P, Nowak C, Eich A, Eckelt J, König H (2011) β-1-3-Glucanase from Delftia tsuruhatensis strain MV01 and its potential application in vinifivation. Appl Environ Microbiol 77:983–990

    Article  PubMed  CAS  Google Scholar 

  • Boido E, Lloret A, Medina K, Carreau F, Dellacassa E (2002) Effect of beta-glycosidase activity of Oenococcus oeni on the glycosylated flavor precursors of Tannat wine during malolactic fermentation. J Agric Food Chem 50:2344–2349

    Article  CAS  PubMed  Google Scholar 

  • Caggianello G, Kleerebezem M, Spano G (2016) Exopolysaccharides produced by lactic acid bacteria: from health-promoting benefits to stress tolerance mechanisms. Appl Microbiol Biotechnol 100:3877–3886

    Article  CAS  Google Scholar 

  • Callejón S, Sendra R, Ferrer S, Pardo I (2014) Identification of a novel enzymatic activity from lactic acid bacteria able to degrade biogenic amines in wine. Appl Microbiol Biotechnol 98:185–198

    Article  PubMed  CAS  Google Scholar 

  • Callejón S, Sendra R, Ferrer S, Pardo I (2016) Cloning and characterization of a new laccase from Lactobacillus plantarum J16 CECT 8944 catalyzing biogenic amine degradation. Appl Microbiol Biotechnol 100:3113–3312

    Article  PubMed  CAS  Google Scholar 

  • Canas PMI, Garcia-Romero E, Manso JMH, Fernandez-Gonzalez M (2014) Influence of sequential inoculation of Wickerhamomyces anomalus and Saccharomyces cerevisiae in the quality of red wines. Eur Food Res Technol 239:27–28

    Google Scholar 

  • Charoenchai C, Fleet GH, Henschke PA, Todd BEN (1997) Screening of non-Saccharomyces wine yeasts for the presence of extracellular hydrolytic enzymes. Aust J Grape Wine Res 3:2–8

    Article  CAS  Google Scholar 

  • Chassagne DS, Vernizeau M, Nedjma M, Alexandre H (2005) Hydrolysis and sorption by Saccharomyces cerevisiae strains of Chardonnay grape must glycosides during fermentation. Enzym Microb Technol 37:212–217

    Article  CAS  Google Scholar 

  • Chasseriaud L, Miot-Sertier C, Coulon J, Iturmendi N, Moine V, Albertin W, Bely M (2015) A new method for monitoring the extracellular proteolytic activity of wine yeasts during alcoholic fermentation of grape must. J Microbiol Methods 119:176–179

    Article  CAS  PubMed  Google Scholar 

  • Christ E, Kowalczyk M, Zuchowska M, Claus H, Löwenstein R, Szopinska-Morawska A, Renaut J, König H (2015) An exemplary model study for overcoming stuck fermentation during spontaneous fermentation with the aid of a Saccharomyces triple hybrid. J Agric Sci 7:18–34

    Google Scholar 

  • Claus H (2007) Extracellular enzymes and peptides of lactic acid bacteria: significance for vinification. Deutsche Lebensmittel-Rundschau 11:505–551

    Google Scholar 

  • Claus H, Filip Z (1988) Behaviour of phenoloxidases in the presence of clays and other soil-related adsorbents. Appl Microbiol Biotechnol 28:506–511

    Article  CAS  Google Scholar 

  • Claus H, Tenzer S, Sobe M, Schlander M, König H, Fröhlich J (2014) Effect of carboxymethyl cellulose on tartrate salt, protein and colour stability of red wine. Aust J Wine Grape Res 20:186–193

    Article  CAS  Google Scholar 

  • Comitini F, Gobbi M, Domizio P, Romani C, Lencioni L, Mannazzu I, Ciani M (2011) Selected non-Saccharomyces wine yeasts in controlled multistarter fermentations with Saccharomyces cerevisiae. Food Microbiol 28:873–882

    Article  CAS  PubMed  Google Scholar 

  • Costello PJ, Siebert TE, Solomon MR, Bartowsky EJ (2013) Synthesis of fruity ethyl esters by acyl coenzyme A: alcohol acyltransferase activities in Oenococcus oeni and Lactobacillus plantarum. J Appl Microbiol 114:797–806

    Article  CAS  PubMed  Google Scholar 

  • D’Incecco N, Bartowsky EJ, Kassara S, Lante A, Spettoli P, Henschke PA (2004) Release of glycosidically bound flavour compounds from Chardonnay by Oenococcus oeni during malolactic fermentaion. Food Microbiol 21:257–265

    Article  CAS  Google Scholar 

  • Daenen L, Saison D, Sterckx F, Delvaux FR, Verachtert H, Derdelelinckx G (2008) Screening and evaluation of the glucoside hydrolase activity in Saccharomyces and Brettanomyces brewing yeasts. Appl Microbiol 104:478–488

    CAS  Google Scholar 

  • Davis CR, Wibowo D, Fleet GH, Lee TH (1988) Properties of wine lactic acid bacteria: their potential enological significance. Am J Enol Vitic 39:137–142

    CAS  Google Scholar 

  • Deckwart M, Carstens C, Webber-Witt M, Schaefer V, Eichhorn L, Schroter F, Fischer M, Brockow K, Christmann M, Paschke-Kratzin A (2014) Impact of wine manufacturing practice on the occurrence of fining agents with allergenic potential. Food Add Contamin Part A – Chem Anal Contr Expo Risk Assess 31:1805–1817

    Article  CAS  Google Scholar 

  • Delaquis P, Cliff M, King B, Girard B, Hall J, Reynolds A (2000) Effect of two commercial malolactic cultures on the chemical and sensory properties of Chancellor wines with different yeasts and fermentation temperatures. Am J Enol Vitic 51:42–48

    CAS  Google Scholar 

  • Delcroix A, Gunata Z, Sapis LC, Salmon JM, Baynone C (1994) Glycosidase activities of three enological yeast strains during winemaking: effect on the terpenol content of Muscat wine. Am J Enol Vitic 45:291–296

    CAS  Google Scholar 

  • Dubourdieu D, Ribéreau-Gayon P, Fournet B (1981) Structure of the extracellular β-D-glucan from Botrytis cinerea. Carbohydr Res 93:294–229

    Article  CAS  Google Scholar 

  • El Rayess Y (2014) Wine: phenolic composition, classification and health benefits. Nova, New York. ISBN: 978-1-63321-048-6

    Google Scholar 

  • Enrique M, Ibanez A, Marcos JF, Yuste M, Martinez M, Valles S, Manzanares P (2010) Beta-Glucanases as a tool for the control of wine spoilage yeasts. J Food Sci 75:M41–M45

    Article  CAS  PubMed  Google Scholar 

  • Eschstruth A, Divol B (2011) Comparative characterization of endo-polygalacturonase (Pgu1) from Saccharomyces cerevisiae and Saccharomyces paradoxus under winemaking conditions. Appl Microbiol Biotechnol 91:623–634

    Article  CAS  PubMed  Google Scholar 

  • Esteban-Torres M, Barcenilla JM, Mancheno JM, de las Rivas B, Munoz R (2014) Characterization of a versatile arylesterase from Lactobacillus plantarum active on wine esters. J Agric Food Chem 62:5118–5125

    Article  CAS  PubMed  Google Scholar 

  • Esti M, Benucci I, Lombardelli C, Liburdi K, Garzillo AMV (2013) Papain from papaya (Carica papaya L.) fruit and latex: preliminary characterization in alcoholic-acidic buffer for wine application. Food Bioprod Process 91:595–598

    Article  CAS  Google Scholar 

  • Farias ME, de Nadra MCM (2000) Purification and partial characterization of Oenococcus oeni exoprotease. FEMS Microbiol Lett 185:263–266

    Article  CAS  PubMed  Google Scholar 

  • Fernández M, Úbeda JF, Briones AI (2000) Typing of non-Saccharomyces yeasts with enzymatic activities of interest in wine-making. Int J Food Microbiol 59:29–36

    Article  PubMed  Google Scholar 

  • Ferreira AM, Climaco MC, Faia AM (2001) The role of non-Saccharomyces species in releasing glycosidic bound fraction of grape aroma components – a preliminary study. J Appl Microbiol 91:67–71

    Article  CAS  Google Scholar 

  • Folio P, Ritt JF, Alexandre H, Remize F (2008) Characterization of EprA, a major extracellular protein of Oenococcus oeni with protease activity. Int J Food Microbiol 127:26–31

    Article  CAS  PubMed  Google Scholar 

  • Fragopoulou E, Antonopoulou S, Demopoulos CA (2002) Biologically active lipids with antiatherogenic properties from white wine and must. J Agric Food Chem 50:2684–2694

    Article  CAS  PubMed  Google Scholar 

  • Friel D, Gomez Pessoa N, Vandenbol M, Jijakli MH (2007) Separate and combined disruptions of two exo-β-1,3-glucanase genes decrease the efficiency of Pichia anomala (strain K) biocontrol against Botrytis cinerea on apples. Mol Plant-Microbe Interact 20:371–379

    Article  CAS  PubMed  Google Scholar 

  • Fugelsang KC, Edwards CG (2007) Wine microbiology: practical applications and procedures, 2nd edn. Springer, Berlin

    Book  Google Scholar 

  • Gallander JF, Peng AC (1980) Lipid and fatty acid composition of different wine grapes. Am J Enol Vitic 31:24–27

    CAS  Google Scholar 

  • Gil JV, Manzanares P, Genoves S, Valles S, Gonzalez-Candelas L (2005) Over-production of the major exoglucanase of Saccharomyces cerevisiae leads to an increase in the aroma of wine. Int J Food Microbiol 103:57–68

    Article  CAS  PubMed  Google Scholar 

  • Girbau T, Stummer BE, Pocock KF, Baldock GA, Scott ES, Waters EJ (2004) The effect of Uncinula necator (powdery mildew) and Botrytis cinerea infection of grapes on the levels of haze-forming pathogenesis-related proteins in grape juice and wine. Aust J Grape Wine Res 10:125–133

    Article  CAS  Google Scholar 

  • Gonzales-Pombo O, Farina L, Carreau F, Batista-Viera F, Brena BM (2011) A novel extracellular beta-glucosidase from Issatchenkia terricola: immobilization and application for aroma enhancement of white Muscat wine. Process Biochem 46:385–389

    Article  CAS  Google Scholar 

  • Gravesse C, Lepoivre P, Jijakli MH (2003) Characterization of the exoglucanase-encoding gene PaEXG2 and study of its role in the biocontrol activity of Pichia anomala strain K. Phytopathology 93:1145–1152

    Article  Google Scholar 

  • Grimaldi A, McLean H, Jiranik V (2000) Identification and partial characterization of glycosidic activities of commercial strains of the lactic acid bacterium Oenococcus oeni. Am J Enol Vitic 51:362–369

    CAS  Google Scholar 

  • Grimaldi A, Bartowsky R, Jiranik V (2005a) A survey of glycosidase activities of commercial wine strains of Oenococcus oeni. Int J Food Microbiol 105:233–244

    Article  CAS  PubMed  Google Scholar 

  • Grimaldi A, Bartowsky E, Jiranik V (2005b) Screening of Lactobacillus spp. and Pediococcus spp. for glycosidase activities that are important in oenology. J Appl Microbiol 99:1061–1069

    Article  CAS  PubMed  Google Scholar 

  • Guilloux-Benatier M, Pageault O, Feuillat M (2000) Lysis of yeast cells by Oenococcus enzymes. J Ind Microbiol Biotechnol 25:193–197

    Article  CAS  Google Scholar 

  • Hjelmeland AK, Ebeler SA (2015) Glycosidically bound volatile aroma compounds in grapes and wine: a review. Am J Enol Vitic 66:1–11

    Article  CAS  Google Scholar 

  • Hu K, Zhu XL, Mu H, Ma Y, Ullah N, Tao YS (2016) A novel extracellular glycosidase activity from Rhodotorula mucilaginosa: its application potential in wine aroma enhancement. Lett Appl Microbiol 62:169–176

    Article  CAS  PubMed  Google Scholar 

  • Iranzo JFU, Pérez AIB, Cañas PMI (1998) Study of oenological characteristics and enzymatic activities of wine yeasts. Food Microbiol 15:399–406

    Article  CAS  Google Scholar 

  • Izzo R, Muratore G (1993) Seed lipids from some varieties of grapes grown in Sicily: note 1. Fatty acid composition. Riv Ital Sostanze Grasse 70:601–604

    CAS  Google Scholar 

  • Jaeckels N, Tenzer S, Rosch A, Scholten G, Decker H, Fronk P (2015) β-Glucosidase removal due to bentonite fining during winemaking. Eur Food Res Technol 241:253–262

    Article  CAS  Google Scholar 

  • Jimenez N, Esteban-Torres M, Miguel Mancheno J, de las Rivas B, Munoz R (2014) Tannin degradation by a novel tannase enzyme present in some Lactobacillus plantarum strains. Appl Environ Microbiol 80:2991–2997

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Jolly NP, Varela C, Pretorius IS (2014) Not your ordinary yeast: non-Saccharomyces yeasts in wine production uncovered. FEMS Yeast Res 14:215–237

    Article  CAS  PubMed  Google Scholar 

  • Lagace LS, Bisson LF (1990) Survey of yeast proteases for effectiveness of wine haze reduction. Am J Enol Vitic 41:147–155

    CAS  Google Scholar 

  • Lambrechts MG, Pretorius IS (2000) Yeast and its importance to wine aroma: a review. S Afr J Enol Vitic 21:97–129

    CAS  Google Scholar 

  • Liburdi K, Benucci I, Esti M (2014) Lysozyme in wine: an overview of current and future applications. Compr Rev Food Sci Food Saf 13:1062–1073

    Article  CAS  Google Scholar 

  • Llaubéres RM, Richard B, Lonvaud A, Dubourdieau D, Fournet B (1990) Structure of extracellular β-D-glucan from Pediococcus sp., a wine lactic bacterium. Carbohydr Res 203:103–107

    Article  PubMed  Google Scholar 

  • López MC, Mateo JJ, Maicas S (2015) Screening of β-glucosidase and β-xylosidase activities in four non-Saccharomyces yeast isolates. J Food Sci 80:C1696–C1704

    Article  PubMed  CAS  Google Scholar 

  • López MC, Mateo JJ, Maicas S (2016) Screening of Hanseniaspora strains for production of enzymes with potential interest for winemaking. Fermentation 2:1. doi:10.3390/fermentation2010001

    Article  Google Scholar 

  • Madrigal T, Maicas S, Tolosa JJM (2013) Glucose and ethanol tolerant enzymes produced by Pichia (Wickerhamomyces) isolates from enological ecosystems. Am J Enol Vitic 64:126–133

    Article  CAS  Google Scholar 

  • Maicas S, Mateo JJ (2016) Microbial glycosidases for wine production. Beverages 2:20. doi:10.3390/beverages2030020

    Article  Google Scholar 

  • Maicas S, Gil JV, Pardo I, Ferrer S (1999) Improvement of volatile composition of wines by controlled addition of malolactic bacteria. Food Res Int 32:491–496

    Article  CAS  Google Scholar 

  • Manca de Nadra MC, Strasser de Saad AM (1995) Polysaccharide production by Pediococcus pentosaceus from wine. Int J Food Microbiol 27:101–106

    Article  CAS  PubMed  Google Scholar 

  • Manca de Nadra MC, Farίas NE, Moreno-Arribas V, Pueyo E, Polo MC (1997) Proteolytic activity of Leuconostoc oneos: effect on proteins and polypeptides from white wine. FEMS Microbiol Lett 150:135–139

    Article  CAS  Google Scholar 

  • Manca de Nadra MC, Farίas NE, Moreno-Arribas V, Pueyo E, Polo MC (1999) A proteolytic effect of Oenococcus oeni on the nitrogenous macromolecular fraction of red wine. FEMS Microbiol Lett 174:491–496

    Article  Google Scholar 

  • Mansfield AK, Zoecklein BW, Whitton RS (2002) Quantification of glycosidase activity in selected strains of Brettanomyces bruxellensis and Oenococcus oeni. Am J Enol Vitic 53:303–307

    CAS  Google Scholar 

  • Marangon M, Van Sluyter SC, Robinson EMC, Muhlack RA, Holt HE, Haynes PA, Godden PW, Smith PA, Waters EJ (2012) Degradation of white wine haze proteins by Aspergillopepsin I and II during juice flash pasteurization. Food Chem 135:1157–1165

    Article  CAS  PubMed  Google Scholar 

  • Marchal R, Berthier L, Legendre L, Marchal-Delahaut L, Jeandet P, Maujean A (1998) Effects of Botrytis cinerea on the must protein electrophoretic characteristics. J Agric Food Chem 46:4945–4949

    Article  CAS  Google Scholar 

  • Marchal R, Warchol M, Cilindre C, Jeandet P (2006) Evidence for protein degradation by Botrytis cinerea and relationships with alteration of synthetic wine properties. J Agric Food Chem 54:5157–5165

    Article  CAS  PubMed  Google Scholar 

  • Mateo JJ, DiStefano R (1997) Description of the beta-glucosidase activity of wine yeasts. Food Microbiol 14:583–591

    Article  CAS  Google Scholar 

  • Mateo JJ, Maicas S, Thießen C (2015) Biotechnological characterisation of extracellular proteases produced by enological Hanseniaspora isolates. Int J Food Sci Technol 50:218–225

    Article  CAS  Google Scholar 

  • Matthews A, Grimaldi A, Walker M, Bartowsky E, Grbin P, Jiranek V (2004) Lactic acid bacteria as a potential source of enzymes for use in vinification. Appl Environ Microbiol 70:5715–5731

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Matthews A, Grbin PR, Jiranek V (2006) A survey of lactic acid bacteria for enzymes of interest to oenology. Aust J Grape Wine Res 12:235–244

    Article  CAS  Google Scholar 

  • Matthews A, Grbin PR, Jiranek V (2007) Biochemical characterisation of the esterase activities of wine lactic bacteria. Appl Microbiol Biotechnol 77:329–337

    Article  CAS  PubMed  Google Scholar 

  • Maturano YP, Assaf LAR, Toro ME, Nally MC, Vallejo M, Castellanos de Figueroa LI, Combina M, Vazquez F (2012) Multi-enzyme production by pure and mixed cultures of Saccharomyces and non-Saccharomyces yeasts during wine fermentation. J Food Microbiol 155:43–50

    Article  CAS  Google Scholar 

  • Maturano YP, Assof M, Fabani MP, Nally MC, Jofré V, Assaf LAR, Toro ME, Castellanos de Figueroa LI, Vazquez F (2015) Enzymatic activities produced by mixed Saccharomyces and non-Saccharomyces cultures: relationship with wine volatile composition. Antonie Van Leeuwenhoek 108:1239–1256

    Article  CAS  PubMed  Google Scholar 

  • Merín MG, de Ambrosini VIM (2015) Highly cold-active pectinases under wine-like conditions from non-Saccharomyces yeasts for enzymatic production during winemaking. Lett Appl Microbiol 60:467–474

    Article  PubMed  CAS  Google Scholar 

  • Merín MG, Mendoza LM, Farías ME, de Ambrosini VIM (2011) Isolation and selection of yeast from wine grape ecosystem secreting cold-active pectinolytic activity. Int J Food Microbiol 147:144–148

    Article  PubMed  CAS  Google Scholar 

  • Michlmayer H, Nauer S, Brandes W, Schumann C, Kulbe KD, del Hierro AM, Eder R (2012) Release of wine monoterpenes from natural precursors by glycosidases from Oenococcus oeni. Food Chem 135:80–87

    Article  CAS  Google Scholar 

  • Mojsov KD, Andronikov D, Janevski A, Jordeva S, Zezova S (2015) Enzymes and wine – the enhanced quality and yield. Adv Technol 4:94–100

    Google Scholar 

  • Molnárova J, Vadkertiová R, Stratilová E (2014) Extracellular enzymatic activities and physiological profiles of yeasts colonizing fruit trees. J Basic Microbiol 51:S74–S84

    Article  CAS  Google Scholar 

  • Mostert TT, Divol B (2014) Investigating the proteins released by yeasts in synthetic wine fermentations. Int J Food Microbiol 171:108–118

    Article  CAS  PubMed  Google Scholar 

  • Mtshali PS, Divol B, van Rensburg P, Du Toit M (2010) Genetic screening of wine-relevant enzymes in Lactobacillus species isolated from South Africa. J Appl Microbiol 108:1389–1397

    Article  CAS  PubMed  Google Scholar 

  • Muccilli S, Restuccia C (2015) Bioprotective role of yeasts. Microorganisms 3:588–611

    Article  PubMed  PubMed Central  Google Scholar 

  • Padilla B, Gil JV, Manzanares P (2016) Past and future of non-Saccharomyces yeasts: from spoilage microorganisms to biotechnological tools for improving wine complexity. Front Microbiol. doi:10.3389/fmicrb.2016.00411

  • Pérez G, Fariña L, Barquet M, Boido E, Gaggero C, Dellacassa E, Carrau F (2011) A quick screening method to identify β-glucosidase activity in native wine yeast strains: application of esculin glycerol agar (EGA) medium. World J Microbiol Biotechnol 27:47–55

    Article  CAS  Google Scholar 

  • Perez-Martin F, Sesena S, Miguel Izquierdo P, Martin R, Llanos Palop M (2012) Screening for glycosidase activities of lactic acid bacteria as a biotechnological tool in oenology. World J Microbiol Biotechnol 28:1423–1432

    Article  CAS  PubMed  Google Scholar 

  • Perez-Martin F, Sesena S, Miguel Izquierdo P, Llanos Palop M (2013) Esterase activity of lactic acid bacteria isolated from malolactic fermentation of red wines. Int J Food Microbiol 163:153–158

    Article  CAS  PubMed  Google Scholar 

  • Pfeiffer P, Schlander M, König H (2007) Detection and production of pyroglutamic acid ethyl ester in wine. Deutsche Lebensmittel-Rundschau 103:8–10

    CAS  Google Scholar 

  • Pueyo E, Martinez-Rodriguez A, Polo MC, Santa-Maria G, Bartomé B (2000) Release of lipids during yeast autolysis in model wine. J Agric Food Chem 48:116–122

    Article  CAS  PubMed  Google Scholar 

  • Quatrini P, Marineo S, Puglia AM (2006) The beta-glucosidase encoding gene from yeast strains isolated from Sicilian musts and wines (DQ010949). Center for Biotechnology Information (NCBI), Bethesda. http://www.ncbi.nlm.nih.gov/. Accessed 15 Oct 2006

  • Ramirez HL, Gómez Brizuela L, Úbeda Iranzo J, Arevalo-Villena M, Briones Pérez AI (2016) Pectinase immobilization on a chitosan-coated chitin support. J Food Process Eng 39:97–104

    Article  CAS  Google Scholar 

  • Rapp A, Mandery H (1986) Wine aroma. Experientia 42:873–884

    Article  CAS  Google Scholar 

  • Reid VJ, Theron LW, du Toit M, Divol B (2012) Identification and partial characterization of extracellular aspartic protease genes from Metschnikowia pulcherrima IWBT Y1123 and Candida apicola IWBT Y1384. Appl Environ Microbiol 78:6838–6849

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rizzi C, Mainente F, Pasini G, Simonato B (2016) Hidden exogenous proteins in wine: problems, methods of detection and related legislation – a review. Czech J Food Sci 34:93–104

    Article  Google Scholar 

  • Rodriguez ME, Lopes CA, Broock M, Valles S, Ramon D, Caballero AC (2004) Screening and typing of Patagonian wine yeasts for glycosidase activities. J Appl Microbiol 96:84–95

    Article  CAS  PubMed  Google Scholar 

  • Rollán GC, Farías ME, Manca de Nadra MC (1993) Protease production by Leuconostoc oenos strains isolated from wine. World J Microbiol Biotechnol 9:587–589

    Article  PubMed  Google Scholar 

  • Rollán GC, Farías ME, Manca de Nadra MC (1995) Characterization of two extracellular proteases from Leuconostoc oenos. World J Microbiol Technol 11:153–155

    Article  Google Scholar 

  • Rollán GC, Farías ME, Strasser de Sand AM, Manca de Nadra MC (1998) Exoprotease activity of Leuconostoc oenos in stress condition. J Appl Microbiol 85:219–233

    Article  Google Scholar 

  • Rollero S, Mouret JR, Sanchez I, Camarasa C, Ortiz-Julien A, Sablayrolles JM, Dequin S (2016) Key role of lipid management in nitrogen and aroma metabolism in an evolved wine yeast strain. Microb Cell Fact 15. doi:10.1186/s12934-016-0434-6

  • Rosi L, Costamagna L, Bertuccioli M (1987) Screening for extracellular acid protease(s) production by wine yeasts. J Inst Brew 93:322–324

    Article  CAS  Google Scholar 

  • Rosi I, Vinella M, Domizio P (1994) Characterization of beta-glucosidase activity in yeasts of oenological origin. J Appl Bacteriol 77:519–527

    Article  CAS  PubMed  Google Scholar 

  • Sabel A, Martens S, Petri A, König H, Claus H (2014) Wickerhamomyces anomalus AS1: a new strain with potential to improve wine aroma. Ann Microbiol 64:483–491

    Article  CAS  Google Scholar 

  • Saerens SMG, Delvaux F, Verstrepen KJ, Van Dijck P, Thevelein JM, Delvaux FR (2008) Parameters affecting ethyl ester production by Saccharomyces cerevisiae during fermentation. Appl Environ Microbiol 74:454–461

    Article  CAS  PubMed  Google Scholar 

  • Sahay S, Hamid B, Singh P, Ranjan K, Chauhan D, Rana RS, Chaurse VK (2013) Evaluation of pectinolytic activities for oenological uses from psychrotrophic yeasts. Lett Appl Microbiol 57:115–121

    Article  CAS  PubMed  Google Scholar 

  • Salazar O, Asenjo JA (2007) Enzymatic lysis of microbial cells. Biotechnol Lett 29:985–994

    Article  CAS  PubMed  Google Scholar 

  • Sarry JE, Günata Z (2004) Plant and microbial glycoside hydrolases: volatile release from glycosidic aroma precursors. Food Chem 87:509–521

    Article  CAS  Google Scholar 

  • Schlander M, Distler U, Tenzer S, Thines E, Claus H (2017) Purification and properties of yeast proteases secreted by Wickerhamomyces anomalus 227 and Metschnikowia pulcherrima 446 during growth in a white grape juice. Fermentation 3:2. doi:10.3390/fermentation3010002

    Article  Google Scholar 

  • Schmidt S, Rainieri S, Witte S, Matern U, Martens S (2011) Identification of a Saccharomyces cerevisiae glucosidase that hydrolyzes flavonoid glycosides. Appl Environ Microbiol 77:1751–1757

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Schwentke J, Sabel A, Petri A, König H, Claus H (2014) The wine yeast Wickerhamomyces anomalus AS1 secretes a multifunctional exo-β-1,3 glucanase with implications for winemaking. Yeast 31:349–359

    Article  CAS  PubMed  Google Scholar 

  • Sebastian P, Herr P, Fischer U, König H (2011) Molecular identification of lactic acid bacteria occurring in must and wine. South Afr J Enol Vitic 32:300–309

    CAS  Google Scholar 

  • Selitrennikoff CP (2001) Antifungal proteins. Appl Environ Microbiol 67:2883–2894

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Spagna G, Barbagallo RN, Palmeri R, Restuccia C, Giudici P (2002) Properties of endogenous beta-glucosidase of a Saccharomyces cerevisiae strain isolated from Sicilian musts and wines. Enzym Microb Technol 31:1030–1035

    Article  CAS  Google Scholar 

  • Strauss MLA, Jolly NP, Lambrechts MG, van Rensburg P (2001) Screening for the production of extracellular hydrolytic enzymes by non-Saccharomyces wine yeasts. J Appl Microbiol 91:182–190

    Article  CAS  PubMed  Google Scholar 

  • Styger G, Prior B, Bauer FF (2011) Wine flavor and aroma. J Ind Microbiol 38:1145–1159

    Article  CAS  Google Scholar 

  • Sumby KM, Grbin PR, Jiranek V (2010) Microbial modulation of aromatic esters in wine: current knowledge and future prospects. Food Chem 121:1–16

    Article  CAS  Google Scholar 

  • Sumby KM, Jiranek V, Grbin PR (2013) Ester synthesis and hydrolysis in an aqueous environment, and strain specific changes during malolactic fermentation in wine with Oenococcus oeni. Food Chem 141:1673–1680

    Article  CAS  PubMed  Google Scholar 

  • Szopinska A, Christ E, Planchon S, König H, Evers D, Renaut J (2016) Stuck at work? Quantitative proteomics of environment wine yeast strains reveals the natural mechanism of overcoming stuck. Proteomics 16:593–608

    Article  CAS  PubMed  Google Scholar 

  • Terrier N, Ponced-Legrand C, Cheynier V (2010) Flavanols, flavonols and dihydroflavonols. In: Morena-Arribas MV, Polo MV (eds) Wine chemistry and biochemistry. Springer, New York, pp 463–507

    Google Scholar 

  • Theron LW, Divol B (2014) Microbial aspartic proteases: current and potential applications in industry. Appl Microbiol Biotechnol 98:8853–8863

    Article  CAS  PubMed  Google Scholar 

  • Thilakarathna SH, Rupasinghe HPV (2013) Flavonoid bioavailability and attempts for bioavailability enhancement. Nutrients 5:3367–3387

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Torresi S, Fragipane MT, Garzillo AMV, Massantini R, Contini M (2014) Effects of a β-glucanase enzymatic preparation on yeast lysis during aging of sparkling wines. Food Res Int 55:83–92

    Article  CAS  Google Scholar 

  • Ugliano M (2010) Enzymes in winemaking. In: Morena-Arribas MV, Polo MC (eds) Wine chemistry and biochemistry. Springer, New York, pp 103–126

    Google Scholar 

  • Ugliano M, Henschke PA (2010) Yeasts and wine flavour. In: Morena-Arribas MV, Polo MV (eds) Wine chemistry and biochemistry. Springer, New York, pp 313–392

    Google Scholar 

  • Ugliano M, Bartowsky EJ, McCarthy J, Moio L, Henschke PA (2006) Hydrolysis and transformation of grape glycosidically bound volatile compounds during fermentation with three Saccharomyces yeast strains. J Agric Food Chem 54:6322–6331

    Article  CAS  PubMed  Google Scholar 

  • Vaillant H, Formisyn P (1996) Purification of the malolactic enzyme from a Leuconostoc oenos strain and use in a membrane reactor for achieving the malolactic fermentation in wine. Biotechnol Appl Biochem 24:217–223

    CAS  Google Scholar 

  • Vakhlu J, Kour A (2006) Yeast lipases: enzyme purification, biochemical properties and gene cloning. Electron J Biotechnol 9. doi:10.2225/vol9-issue1-fulltext-9

  • Van Rensburg P, Pretorius IS (2000) Enzymes in winemaking: harnessing natural catalysts for efficient biotransformation – a review. S Afr J Enol Vitic 21:52–73

    Google Scholar 

  • Van Sluyter SC, Warnock NL, Schmidt S, Anderson P, van Kan JAL, Bacic A, Waters EJ (2013) Aspartic acid protease from Botrytis cinerea removes haze-forming proteins during white wine-making. J Agric Food Chem 61:9705–9711

    PubMed  Google Scholar 

  • Van Sluyter SC, McRae JM, Falconer RJ, Smith PA, Bacic A, Waters EJ, Marangon M (2015) Wine protein haze: mechanisms of formation and advances in prevention. J Agric Food Chem 63:4020–4030

    Article  PubMed  CAS  Google Scholar 

  • Van Wyk H, Divol B (2010) Recovery of endo-polygalacturonase activity in wine yeast and its effect of wine aroma. FEMS Yeast Res 10:58–71

    Article  PubMed  CAS  Google Scholar 

  • Vaquero I, Macrobal Á, Muñoz R (2004) Tannase activity by lactic bacteria isolated from grape must and wine. Int J Food Microbiol 96:199–204

    Article  CAS  PubMed  Google Scholar 

  • Velasco SE, Yebra MJ, Monedero V, Ibarburu I, Dueñas MT, Irastorza A (2007) Influence of the carbohydrate source on β-glucan production and enzyme activities involved in sugar metabolism in Pediococcus parvulus 2.6. Int J Food Microbiol 115:325–334

    Article  CAS  PubMed  Google Scholar 

  • Veza S, Rodríguez-Perez R, Carretero P, Juste S, Caballero ML (2015) Occupational allergic bronchial asthma induced by Lallzyme EX-V, an enzymatic blend sourced from Aspergillus niger used as additive in the wine industry. Occup Environ Med 72:237–223

    Article  PubMed  Google Scholar 

  • Villetaz JC, Steiner D, Trogus H (1984) The use of β-glucanase as an enzyme in wine clarification and filtration. Am J Enol Vitic 35:253–256

    Google Scholar 

  • Vivas NA, Lonvaud-Funel A, Glories Y (1997) Effect of phenolic acids and anthocyanins on growth, viability and malolactic activity of a lactic bacterium. Food Microbiol 14:291–300

    Article  CAS  Google Scholar 

  • Wang Y, Zhang C, Xu Y, Li J (2015) Evaluating potential applications of indigenous yeasts and their β-glucosidases. J Inst Brew 121:642–648

    Article  CAS  Google Scholar 

  • Wibowo D, Eschenbruch R, Davis CR, Fleet GH, Lee TH (1985) Occurrence and growth of lactic bacteria in wine. Am J Enol Vitic 36:302–313

    CAS  Google Scholar 

  • Wigand P, Tenzer S, Schild H, Decker H (2009) Analysis of protein composition of red wine in comparison with rosé and white wines by electrophoresis and high-pressure liquid chromatography (HPLC-MS). J Agric Food Chem 57:4328–4333

    Article  CAS  PubMed  Google Scholar 

  • Winterhalter P, Skouroumounis GK (1997) Glycoconjugated aroma compounds: occurrence, role and biotechnological transformation. Adv Biochem Eng Biotechnol 55:74–99

    Google Scholar 

  • Yanai T, Sato N (1999) Isolation and properties of β-glucosidase produced by Debaryomyces hansenii and its application in winemaking. Am J Enol Vitic 50:231–235

    CAS  Google Scholar 

  • Yao J, Guo GS, Ren GH, Liu YH (2014) Production, characterization and applications of tannase. J Mol Catal B Enzym 101:137–147

    Article  CAS  Google Scholar 

  • Younes B, Cilindre C, Villaume S, Parmentier M, Jeandet P, Vasserot Y (2011) Evidence for an extracellular and proteolytic activity secreted by living cells of Saccharomyces cerevisiae PIR1: impact on grape proteins. J Agric Food Chem 59:6239–6246

    Article  CAS  PubMed  Google Scholar 

  • Younes B, Cilindre C, Jeandet P, Vasserot Y (2013) Enzymatic hydrolysis of thermos-sensitive grape proteins by a yeast protease as revealed by a proteomic approach. Food Res Int 54:1298–1301

    Article  CAS  Google Scholar 

  • Younis OS, Stewart GG (1998) Sugar uptake and subsequent ester and higher alcohol production by Saccharomyces cerevisiae. J Inst Brew 104:255–264

    Article  CAS  Google Scholar 

  • Zeemann WJ, Snyman P, van Wyk SJ (1982) The influence of yeast strain and malolactic fermentation on some volatile bouquet substances and on quality of table wines. In: Proceedings of University of California, Davis, Grape and Wine Centennial Symposium, 1980, pp 242–271

    Google Scholar 

  • Zinnai A, Venturi F, Quartacci MF, Andreotti A, Andrich G (2010) The kinetic effect of some wine components on the enzymatic hydrolysis of β-glucan. S Afr J Enol Vitic 31:9–13

    CAS  Google Scholar 

  • Zivkovic K, König H, Claus H (2011) Wirkung von Bentonit auf die Laccase-Aktivität in Most und Wein. Deutsche Lebensmittel Rundschau 107:575–582

    CAS  Google Scholar 

  • Zoecklein B, Marcy J, Williams S, Jasinski Y (1997) Effect of native yeasts and selected strains of Saccharomyces cerevisiae on glycosyl glucose, potential volatile terpenes, and selected aglycons of white Riesling (Vitis vinifera L.) wines. J Food Compos Anal 10:55–65

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Harald Claus .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer International Publishing AG

About this chapter

Check for updates. Verify currency and authenticity via CrossMark

Cite this chapter

Claus, H. (2017). Microbial Enzymes: Relevance for Winemaking. In: König, H., Unden, G., Fröhlich, J. (eds) Biology of Microorganisms on Grapes, in Must and in Wine. Springer, Cham. https://doi.org/10.1007/978-3-319-60021-5_13

Download citation

Publish with us

Policies and ethics