Skip to main content

Botanical Classification

  • Chapter
  • First Online:
Bee Products - Chemical and Biological Properties

Abstract

The study of the pollen content of honeys allows to establish their botanical and geographical origin, contributing significantly to the quality control processes of this hive product. The most relevant characters for identification of pollen grains are: polarity and symmetry, size, shape, sporoderm ornamentation, and number, type and position of apertures. The methodology used has undergone modifications over the years, however Erdtman acetolysis (a method proposed in the last century) is still employed almost worldwide, since most of pollen descriptions in the palynological literature are based on acetolysed material. In the presentation of the results, considering quantitative analysis, it should be indicated to which class of Maurizio the studied honey belongs. However, the qualitative analysis provides the most relevant information. To assess the monoflorality of a honey type, relative frequency of each pollen type, as the percentage with respect to the total number of pollen grains counted/identified (from nectariferous species only), should be included. Nectar honeys are considered as coming predominately from a given botanical origin (monofloral) if the relative frequency of the pollen type of that taxon exceeds 45%. Honeys with over-represented pollen types, such as Eucalyptus spp., or under-represented ones (e.g. Citrus spp.), should reach percentages greater or lower than this value, respectively. Honeydew honeys are poor in pollen grains, whereas the presence of honeydew elements (hyphae, microscopic algae, fungal spores, etc.) is relevant. It is considered to be predominately honeydew if the ratio honeydew elements/pollen grains exceeds 3.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Atanassova J, Pavlova D, Lazarova M, Yurukova L (2016) Characteristics of honey from Serpentine area in the Eastern Rhodopes Mt., Bulgaria. Biol Trace Elem Res 173:247–258

    Article  CAS  PubMed  Google Scholar 

  • Belay A, Solomon WK, Bultossa G, Adgaba N, Melaku S (2015) Botanical origin, color, granulation, and sensory properties of the Harenna forest honey, Bale, Ethiopia. Food Chem 167:213–219

    Article  CAS  PubMed  Google Scholar 

  • Codex Alimentarius Commission (2001) Revised Codex Standard for Honey, Codex STAN 12–1981, Rev. 1 (1987), Rev. 2 (2001)

    Google Scholar 

  • Conceição Silva AP, Ribeiro Dos Santos FA (2014) Pollen diversity in honey from Sergipe, Brazil. Grana 53(2):159–170

    Article  Google Scholar 

  • Corvucci F, Nobili L, Melucci D, Grillenzoni FV (2015) The discrimination of honey using melissopalynology and Raman spectroscopy techniques coupled with multivariate analysis. Food Chem 169:297–304

    Article  CAS  PubMed  Google Scholar 

  • Directive 2014/63/EU of the European Parliament and of the Council of 15 May 2014 amending Council Directive 2001/110/EC relating to honey. OJEU L164:1–5.

    Google Scholar 

  • Erdtman G (1943) An introduction to pollen analysis. The Ronald Press Co, New York, pp 1–239

    Google Scholar 

  • Erdtman G (1952) Pollen morphology and plant taxonomy. Angiosperms. Almqvist and Wiksell, Stockholm, pp 1–539

    Google Scholar 

  • Erdtman G (1960) The acetolysis method. Sven Bot Tidskr 54:561–564

    Google Scholar 

  • Erdtman G (1966) A propos de la stratification de l’exine. Pollen Spores 8:5–7

    Google Scholar 

  • Erdtman G (1969) Handbook of palynology—an introduction to the study of pollen grains and spores. Hafner Publishing Co, Copenhague, pp 1–486

    Google Scholar 

  • EU Council (2002) 2001/110/EC of 20 December 2001 relating to honey. OJEC L10:47–52

    Google Scholar 

  • Fægri K (1956) Recent trends in palynology. Bot Rev 22:639–664

    Article  Google Scholar 

  • Fægri K, Iversen J (1950) Textbook of modern pollen analysis. Munksgaard, Copenhagen, pp 1–169

    Google Scholar 

  • Fernández I, Díez M (1990) Algunas consideraciones sobre terminología palonológica. I, Polaridad y simetría. Lagascalia 16(1):51–60

    Google Scholar 

  • Hideux M (1972) Techniques d’étude du pollen an MED: effects comparés des différents traitements physicochimiques. Micron 3:1–31

    Article  Google Scholar 

  • Hyde HA, Williams DA (1944) The right word. In: Sears PB (ed) Pollen analysis circular no. 8. Department of Botany, Oberlin College, Oberlin, Ohio

    Google Scholar 

  • Juan-Borrás M, Periche A, Domenech E, Escriche I (2015) Correlation between methyl antranilate level and percentage of pollen in Spanish citrus honey. Int J Food Sci Technol 50:1690–1696

    Article  Google Scholar 

  • Kadri SM, Zaluski R, Pereira Lima GP, Mazzafera P, de Oliveira Orsi R (2016) Characterization of Coffea arabica monofloral honey from Espírito Santo, Brazil. Food Chem 203:252–257

    Article  CAS  PubMed  Google Scholar 

  • Knoll M, Ruska E (1932) Das Elektronenmikroskop. Z Phys 78:318

    Article  CAS  Google Scholar 

  • La Sena-Ramos I, Méndez Pérez B, Gómez Ferreras C (2002) Pollen spectra of different unifloral honeys from La Palma (Canary Islands, Spain). Grana 41:48–57

    Article  Google Scholar 

  • Louveaux J, Maurizio A, Vorwhol G (1978) Methods of melissopalynology. Bee World 59(4):139–157

    Article  Google Scholar 

  • Maurizio A (1939) Untersuchungen zur quantitativen pollenlyse des honing. Mitt Lebensmittelunters Hyg 30:27–69

    Google Scholar 

  • Maurizio A (1975) Microscopy of honey. In: Crane E (ed) Honey: a comprehensive survey. Heinemann in cooperation with the International Bee Research Association, London, pp 240–257

    Google Scholar 

  • Ohe WVD, Persano Oddo L, Piana ML, Morlot M, Martin P (2004) Harmonized methods of melissopalynology. Apidologie 35:S18–S25

    Article  Google Scholar 

  • Orden de 12 de junio de (1986) por la que se aprueban los métodos oficiales de análisis para la miel. BOE 145:22195–22202

    Google Scholar 

  • Orden de 5 de agosto de (1983) por la que se aprueba la norma de calidad para la miel destinada al mercado interior. BOE 193:22384–22386

    Google Scholar 

  • Persano Oddo L, Bogdanov S (2004) Determination of honey botanical origin: problems and issues. Apidologie 35:S2–S3

    Article  Google Scholar 

  • Persano Oddo L, Piro R (2004) Main European unifloral honeys: descriptive sheets. Apidologie 35(Suppl. 1):S38–S81

    Article  Google Scholar 

  • Persano Oddo L, Piazza MG, Sabatini AG, Accorti M (1995) Characterization of unifloral honeys. Apidologie 26:453–465

    Article  Google Scholar 

  • Persano Oddo L, Piana L, Bogdanov S, Bentabol A, Gotsiou P, Kerkvliet J, Martin P, Morlot M, Ortiz Valbuena A, Ruoff K, Ohe KVD (2004) Botanical species giving unifloral honey in Europe. Apidologie 35:S82–S93

    Article  Google Scholar 

  • Piazza MG, Persano Oddo L (2004) Bibliographical review of the main European unifloral honeys. Apidologie 35:S94–S111

    Article  Google Scholar 

  • Ponnuchamy R, Bonhomme V, Prasad S, Das L, Patel P, Gaucherel C, Pragasam A, Anupama K (2014) Honey pollen: using melissopalynology to understand foraging preferences of bees in Tropical South India. PLoS One 9(7):e101618

    Article  PubMed  PubMed Central  Google Scholar 

  • Real Decreto 1049/2003, de 1 de agosto, por el que se aprueba la Norma de calidad relativa a la miel. BOE 186:30181–30183.

    Google Scholar 

  • Reitsma TJ (1970) Suggestions towards unification of descriptive terminology of Angiosperm pollen grains. Rev Palaeobot Palynol 10:39–60

    Article  Google Scholar 

  • Rodríguez de la Cruz D, Sánchez Reyes E, Sánchez Durán S, Sánchez Sánchez J (2013) Análisis palinológico de mieles comerciales monoflorales. Botanica Complutensis 37:171–180

    Google Scholar 

  • SAGPyA (Secretaría de Agricultura, Ganadería, Pesca y Alimentación) (1998) Miel, Buenas prácticas de manufactura. Guía de aplicación. Normas y legislación vigentes. Resolución 274(95):72–73

    Google Scholar 

  • Sánchez Sánchez J, Baldi Coronel B (2010) Características microscópicas y origen botánico. In: Baldi B (ed) La miel. Una mirada científica. EDUNER. Universidad Nacional de Entre Ríos, Córdoba, pp 1–221

    Google Scholar 

  • Schievano E, Finotello C, Mammi S, Belci AI, Colomban S, Navarini L (2015) Preliminary characterization of monofloral Coffea spp. honey: correlation between potential biomarkers and pollen content. J Agric Food Chem 63:5858–5863

    Article  CAS  PubMed  Google Scholar 

  • Simeão CMG, Silveira FA, Sampaio IBM, Bastos EMAF (2015) Pollen analysis of honey and pollen collected by Apis mellifera linnaeus, 1758 (Hymenoptera, Apidae), in a mixed environment of Eucalyptus plantation and native cerrado in Southeastern Brazil. Braz J Biol 75(4):821–829

    Article  PubMed  Google Scholar 

  • Soria AC, González M, de Lorenzo C, Martínez-Castro I, Sanz J (2004) Characterization of artisanal honeys from Madrid (Central Spain) on the basis of their melissopalynological, physicochemical and volatile composition data. Food Chem 85:121–130

    Article  CAS  Google Scholar 

  • Wodehouse RP (1935) Pollen grains. Their structure, identification and significance in science and medicine. McGraw-Hill, New York, pp 1–574

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Estefanía Sánchez Reyes .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer International Publishing AG

About this chapter

Cite this chapter

Reyes, E.S., Sanchéz, J.S. (2017). Botanical Classification. In: Alvarez-Suarez, J. (eds) Bee Products - Chemical and Biological Properties. Springer, Cham. https://doi.org/10.1007/978-3-319-59689-1_1

Download citation

Publish with us

Policies and ethics