Laboratory Diagnosis

  • Marta Díaz-MenéndezEmail author
  • Clara Crespillo-Andújar
Part of the SpringerBriefs in Immunology book series (BRIEFSIMMUN)


ZIKV infections overlap clinical signs and symptoms with other arboviriasis, so microbiologic diagnosis may be necessary to specify final diagnosis. There are different microbiologic techniques that lead to the diagnosis on ZIKV infection. The viral genome detection in blood or urine by molecular techniques provides an accurate diagnostic result, but viremia is detectable only briefly during acute illness in these specimens. Later on, serology is the most commonly used diagnostic method for ZIKV infection. Serological cross-reactions in secondary infection with closely related flavivirus (i.e., dengue or West Nile virus) as well as vaccines to flavivirus (i.e., Yellow fever or Japanese encephalitis vaccine) are possible. Neutralising antibody detection assays can elucidate if serological positive tests are due to ZIKV or to other flavivirus. ZIKV culture is not usually available outside research laboratories. Diagnostics test are recommended to those symptomatic patients from endemic areas of ZIKV, and for all pregnant regardless they had symptoms or not. Congenital syndrome newborns also should be tested for ZIKV. Patient samples must be handled according to the biosafety guidelines in microbiological and medical laboratories.


Reverse Transcriptase Polymerase Chain Reaction West Nile Virus Japanese Encephalitis ZIKV Infection Plaque Reduction Neutralization Test 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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© The Author(s) 2017

Authors and Affiliations

  • Marta Díaz-Menéndez
    • 1
    Email author
  • Clara Crespillo-Andújar
    • 1
  1. 1.Tropical Medicine DepartmentHospital Universitario La Paz-Carlos IIIMadridSpain

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