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Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products pp 79–96Cite as

Aqueous Two-Phase System Strategies for the Recovery and Partial Purification of Bioactive Low Molecular Weight Compounds

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Part of the book series: Food Engineering Series ((FSES))

Abstract

In the last two decades, there has been an increase in the market of bioactive low molecular weight compounds (LMWCs) due to their wide applications. This has motived the development and application of different methods to perform their extraction, recovery, and purification, taking into consideration strategies that have advantages over traditional extraction techniques. In this context, aqueous two-phase systems (ATPS) represent an attractive alternative to conventional downstream processing techniques. Although the studies related to the partition of biomolecules in ATPS have mainly focused on macrobiomolecules (proteins, nucleic acids, etc.) and bioparticles (viruses, viruslike particles, organelles, cells, etc.), reports in literature demonstrate the great potential of ATPS for the recovery and partial purification of LMWC. The aim of this chapter is to review the application of ATPS for the extraction, recovery, and partial purification of low molecular weight compounds. Selected studies presenting the state-of-the-art in the topic are discussed, and finally future trends in the use of ATPS for the fractionation of LMWC are envisioned.

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Abbreviations

ATPS:

Aqueous two-phase systems

diCQA:

Dicaffeoylquinic acids

[EMIM][Ac]:

1-ethyl-3-methylimidazolium acetate

HPLC:

High-performance liquid chromatography

LMWCs:

Low molecular weight compounds

SFE:

Supercritical fluid extraction

SPE:

Solid-phase extraction

TLL:

Tie-line length

V R :

Volume ratio

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Authors and Affiliations

  1. Universidad de Colima, Campus Tecomán, Autopista Colima-Manzanillo km. 40, Tecomán, Col. 28100, México

    Juan Carlos Sánchez-Rangel

  2. Tecnológico de Monterrey, Escuela de Ingeniería y Ciencias, Centro de Biotecnología-FEMSA, Ave. Eugenio Garza Sada 2501, Monterrey, NL, 64849, México

    Daniel A. Jacobo-Velázquez & Jorge Benavides

Authors
  1. Juan Carlos Sánchez-Rangel
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  2. Daniel A. Jacobo-Velázquez
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  3. Jorge Benavides
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Corresponding author

Correspondence to Jorge Benavides .

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Editors and Affiliations

  1. Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias Centro de Biotecnología-FEMSA, Monterrey, Nuevo León, Mexico

    Marco Rito-Palomares

  2. Tecnologico de Monterrey, Escuela de Medicina, Monterrey, Nuevo León, Mexico

    Jorge Benavides

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Sánchez-Rangel, J.C., Jacobo-Velázquez, D.A., Benavides, J. (2017). Aqueous Two-Phase System Strategies for the Recovery and Partial Purification of Bioactive Low Molecular Weight Compounds. In: Rito-Palomares, M., Benavides, J. (eds) Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products. Food Engineering Series. Springer, Cham. https://doi.org/10.1007/978-3-319-59309-8_5

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