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Practical Aspects for the Development of ATPS-Based Processes for Protein Recovery

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Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products

Part of the book series: Food Engineering Series ((FSES))

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Abstract

The selection and implementation of a successful aqueous two-phase system (ATPS) strategy requires an extensive amount of research to optimize the different system design parameters to obtain the desired product yields and purity. This procedure might become even more difficult depending on the complexity of the sample being processed. However, because of their characteristics, ATPS represent an interesting strategy for the recovery of different proteic products from a wide array of available sources.

The aim of this chapter is to highlight the different alternatives – from a practical point of view – in the selection of different ATPS to serve as a guide for the correct design and use of these operations for the recovery of protein molecules. In this context, our group has been working with these strategies for more than two decades. Our experience ranges from the ATPS extraction of proteins from simple sources to the extraction of these molecules from complex industrial wastes. In this chapter, we present a general and successful strategy that has served us as a first approach in implementing ATPS operations; we also present several of our most recent attempts in using ATPS in an intensive manner for the extraction of high added-value proteins from waste streams, chemical reactions, and the refolding of denatured proteins.

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Abbreviations

ATPS:

Aqueous two-phase systems

CI2:

Chymotrypsin inhibitor 2

HTS:

High-throughput screening

K P :

Partition coefficient

MW:

Molecular weight

PEG:

Polyethylene glycol

pI:

Isoelectric point

PPO-Ph:

Poly(propylene oxide)-phenyl

RNase A:

Ribonuclease A

TLL:

Tie-line length

UCON:

Ethylene oxide and propylene oxide

V R :

Volume ratio

α-Lac:

α-lactalbumin

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Authors and Affiliations

  1. Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Centro de Biotecnología-FEMSA, Ave. Eugenio Garza Sada 2501, Monterrey, NL, 64849, México

    José González-Valdez & Karla Mayolo-Deloisa

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  1. José González-Valdez
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  2. Karla Mayolo-Deloisa
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Correspondence to José González-Valdez .

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Editors and Affiliations

  1. Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias Centro de Biotecnología-FEMSA, Monterrey, Nuevo León, Mexico

    Marco Rito-Palomares

  2. Tecnologico de Monterrey, Escuela de Medicina, Monterrey, Nuevo León, Mexico

    Jorge Benavides

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González-Valdez, J., Mayolo-Deloisa, K. (2017). Practical Aspects for the Development of ATPS-Based Processes for Protein Recovery. In: Rito-Palomares, M., Benavides, J. (eds) Aqueous Two-Phase Systems for Bioprocess Development for the Recovery of Biological Products. Food Engineering Series. Springer, Cham. https://doi.org/10.1007/978-3-319-59309-8_3

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