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Metabolomics

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Perspectives in Sustainable Nematode Management Through Pochonia chlamydosporia Applications for Root and Rhizosphere Health

Part of the book series: Sustainability in Plant and Crop Protection ((SUPP))

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Abstract

Metabolomics is the study of metabolites, small biomolecules (carbohydrates, lipids, amino acids and organic acids) present in a biological sample. Metabolomics tools include chromatography for separating metabolites and spectroscopy techniques for their identification. Metabolomics tools have allowed to analyze the composition of tomato root exudates in the tritrophic system: Pochonia chlamydosporia, Meloidogyne javanica and tomato (Solanum lycopersicum) and changes in root exudates that were due to the presence of the fungus, the nematode or both. Large amounts of fluorescent compounds were detected in tomato root exudates from plants with M. javanica root galls and egg masses. Profiles of root exudates in 1H NMR included organic acids, sugars and amino acids. Acetate signal increased in root exudates with M. javanica. Using HPLC-MS metabolomic fingerprints of tomato root exudates were generated. Several m/z signals have been found and related to the presence of M. javanica and only one with the presence of P. chlamydosporia. Metabolomics data integrated with transcriptomics will help to understand rhizosphere signalling in multitrophic systems.

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Acknowledgements

This research was funded by the Spanish Ministry of Economy and Competitiveness Grant AGL 2015-66833-R. Luis Vicente Lopez-Llorca was awarded a sabbatical grant (PR2015_00087) by the Spanish Ministry of Education, Culture and Sport.

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Authors and Affiliations

  1. Laboratory of Plant Pathology, Department of Marine Sciences and Applied Biology, University of Alicante, Alicante, Spain

    Nuria Escudero, Frutos Marhuenda-Egea & Luis V. Lopez-Llorca

  2. Department of Marine Sciences and Applied Biology, Multidisciplinary Institute for Environmental Studies (MIES) Ramón Margalef, University of Alicante, Alicante, Spain

    Nuria Escudero, Frutos Marhuenda-Egea & Luis V. Lopez-Llorca

  3. Department of Agri-Food Engineering and Biotechnology, Universitat Politècnica de Catalunya, Catalunya, Spain

    Nuria Escudero

  4. Department of Agrochemistry and Biochemistry, University of Alicante, Alicante, Spain

    Frutos Marhuenda-Egea

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Correspondence to Nuria Escudero .

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  1. Centro de Desarrollo de Productos Bióticos (Visiting Professor), Instituto Politécnico Nacional (CEPROBI), Yautepec, Morelos, Mexico

    Rosa H. Manzanilla-López

  2. Department of Marine Sciences and Applied Biology, University of Alicante, Alicante, Spain

    Luis V. Lopez-Llorca

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Escudero, N., Marhuenda-Egea, F., Lopez-Llorca, L.V. (2017). Metabolomics. In: Manzanilla-López, R., Lopez-Llorca, L. (eds) Perspectives in Sustainable Nematode Management Through Pochonia chlamydosporia Applications for Root and Rhizosphere Health. Sustainability in Plant and Crop Protection. Springer, Cham. https://doi.org/10.1007/978-3-319-59224-4_8

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