Abstract
Enzyme immobilization onto nanomaterials has been implemented in various fields such as water decontamination, sensor developments, biotransformation, therapeutics, foods processing, biofuel production, and so on. In this study, we aimed to covalently immobilize 3,4-POD onto H2SO4 and HNO3 functionalized (F)-MWCNTs to birth Nanobiohybrid catalyst. Images of SEM, TEM, and AFM along with UV/vis and IR spectroscopic data demonstrated that the 3,4-POD was successfully immobilized onto F-MWCNT surfaces. CD spectroscopy data showed that the Nanobiohybrid undergone 44% of relative structural changes to its free 3,4-POD configurations. Optimizing immobilization parameters, such as the use of cross-linker, time incubation, and different concentrations of 3,4-POD loading helped us to attach maximum 1060 µg of 3,4-POD/mg of MWCNT. This paves the way for the development of effective Nanobiohybrid that might have the imminent potentiality to purify 3,4-DHBA contaminated wastewater.
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Das, R. (2017). Nanobiohybrid Preparation. In: Nanohybrid Catalyst based on Carbon Nanotube. Carbon Nanostructures. Springer, Cham. https://doi.org/10.1007/978-3-319-58151-4_5
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