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Perioperative Inflammation as Triggering Origin of Metastasis Development pp 109–122Cite as

Circulating Tumor Cells as Predictive Marker in Metastatic Disease

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Abstract

Numerous studies have demonstrated that circulating tumor cell (CTC) number in peripheral blood correlates with therapy efficiency in various tumors, and can serve as a prognostic marker of metastasis development. Recent clinical data support many oncologists’ opinion that some medical procedures may provoke metastasis by triggering increased tumor cell shedding into circulation, but no systematic study has been performed. The development of an in vivo flow cytometry method for real-time CTC quantification can provide insights on CTC release dynamics during different medical interventions and possibly long and short term outcome predictions.

Noninvasive enumeration of CTCs is the most direct way to confirm the central hypothesis that tumor manipulations during medical interventions can promote liberation of CTC into the bloodstream. Incisional biopsy and complete tumor resection in melanoma-bearing mice were conducted and the CTC rate was monitored before, during, and for a short-term after the procedures. Incisional biopsy significantly increased CTC counts (up to 60-fold), whereas complete tumor resection significantly decreased CTC counts. Long-term in vivo monitoring of CTC triggered by punch biopsy and complete tumor resection was performed on breast cancer- bearing mice. After punch biopsy, the number of CTC increased. In contrast, complete tumor resection significantly decreased the CTC count. New techniques were proposed for labeling newly released CTCs in order to identify them among previously circulating cells.

These findings have broad clinical implications to reduce viable CTCs release during diagnostics and treatments by real-time monitoring of CTC dynamics followed by well-timed treatment to reduce CTCs in the blood.

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Author information

Authors and Affiliations

  1. Department of General and Visceral Surgery, Goethe-University Hospital of Frankfurt, Frankfurt am Main, Germany

    Mazen A. Juratli

  2. Winthrop P. Rockefeller Cancer Institute, Arkansas Nanomedicine Center, University of Arkansas for Medical Sciences (UAMS), 4301 West Markham, Little Rock, AR, 72205, USA

    Mazen A. Juratli, Dmitry A. Nedosekin, Mustafa Sarimollaoglu, Eric R. Siegel, Ekaterina I. Galanzha & Vladimir P. Zharov

Authors
  1. Mazen A. Juratli
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  2. Dmitry A. Nedosekin
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  3. Mustafa Sarimollaoglu
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  4. Eric R. Siegel
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  5. Ekaterina I. Galanzha
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  6. Vladimir P. Zharov
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Corresponding author

Correspondence to Mazen A. Juratli .

Editor information

Editors and Affiliations

  1. Harvard TH Chan School of Public Health, Boston, Massachusetts, USA

    Michael W. Retsky

  2. Istituto Nazionale Tumori di Milano, Fondazione IRCCS, Milan, Italy

    Romano Demicheli

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Juratli, M.A., Nedosekin, D.A., Sarimollaoglu, M., Siegel, E.R., Galanzha, E.I., Zharov, V.P. (2017). Circulating Tumor Cells as Predictive Marker in Metastatic Disease. In: Retsky, M., Demicheli, R. (eds) Perioperative Inflammation as Triggering Origin of Metastasis Development. Springer, Cham. https://doi.org/10.1007/978-3-319-57943-6_5

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