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Grapevine fleck and similar viruses

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Grapevine Viruses: Molecular Biology, Diagnostics and Management

Abstract

Grapevine fleck virus (GFkV), grapevine red globe virus (GRGV), grapevine rupestris vein feathering virus (GRVFV), grapevine asteroid mosaic-associated virus (GAMaV), and grapevine Syrah virus 1 (GSyV-1) are a group of evolutionarily related viruses with similar morphological, physicochemical, and molecular properties. GFkV is the agent of fleck disease, and GAMaV and GRVFV are associated with asteroid mosaic and vein feathering diseases, respectively, while GRGV is not involved in any specific symptomatology. GSyV-1 is included in this chapter as it shares many traits with the four aforementioned viruses, but has not yet been associated with any particular syndrome. All these viruses are phloem limited, nonmechanically transmissible, and primarily spread through infected propagating material. GFkV is ubiquitous, while the other viruses have been reported only from certain geographical areas. Despite few unconfirmed reports of natural field transmission of GFkV, no vector has been identified for it, nor any of the other viruses. Viral genomes consist of a single molecule of a capped and polyadenylated, cytosine-rich, messenger-like RNA 6.5–7.5 kb in size. According to the current taxonomy, these viruses belong to recognized or putative species in the genera Maculavirus (GFkV and GRGV) and Marafivirus (GSyV-1, GRVFV, and GAMaV) in the family Tymoviridae, order Tymovirales.

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References

  • Abou Ghanem-Sabanadzovic, N., S. Sabanadzovic, and G.P. Martelli. 2003a. Sequencing of the 3′ end of three grapevine fleck virus-like viruses. Virus Genes 27: 11–16.

    Article  Google Scholar 

  • Abou Ghanem-Sabanadzovic, N., S. Sabanadzovic, A. Rowhani, and G.P. Martelli. 2003b. Multiplex RT-PCR detection of grapevine fleck virus-like viruses in grapevine with coamplification of control plant mRNA. Extended abstracts 14th meeting of ICVG, Locorotondo, Italy: 195.

    Google Scholar 

  • Al Rwahnih, M., S. Daubert, D.A. Golino, and A. Rowhani. 2009. Deep sequencing analysis of RNAs from a grapevine showing Syrah decline symptoms reveals a multiple virus infection that included a novel virus. Virology 387: 395–401.

    Article  CAS  PubMed  Google Scholar 

  • Barlass, M., K.G.M. Skene, R.C. Woodham, and L.R. Krake. 1982. Regeneration of virus-free grapevines using in vitro apical culture. Annals of Applied Biology 101: 291–295.

    Google Scholar 

  • Bertolini, E., J. Garcia, A. Yuste, and A. Olmos. 2010. High prevalence of viruses in table grape from Spain detected by real-time RT-PCR. European Journal of Plant Pathology 128: 283–287.

    Article  Google Scholar 

  • Beuve, M., T. Candresse, M. Tannières, and O. Lemaire. 2015. First report of grapevine redglobe virus in grapevine in France. Plant Disease 99: 422.

    Article  Google Scholar 

  • Boscia, D., G.P. Martelli, V. Savino, and M.A. Castellano. 1991a. Identification of the agent of grapevine fleck disease. Vitis 30: 97–105.

    Google Scholar 

  • Boscia, D., V. Savino, G.P. Martelli, and M.A. Castellano. 1991b. Association of a phloem-limited non mechanically transmissible isometric virus with grapevine fleck disease. Proceedings 10th meeting of ICVG, Volos, Greece: 173–174.

    Google Scholar 

  • Boscia, D., S. Sabanadzovic, V. Savino, P.E. Kyriakopoulou, and G.P. Martelli. 1994. A non mechanically transmissible virus associated with asteroid mosaic of the grapevine. Vitis 33: 101–102.

    Google Scholar 

  • Boscia, D., V. Elicio, V. Savino, and G.P. Martelli. 1995. Production of monoclonal antibodies to grapevine fleck virus. Plant Pathology 44: 160–163.

    Google Scholar 

  • Boulila, M., D. Boscia, B. Di Terlizzi, M.A. Castellano, A. Minafra, V. Savino, and G.P. Martelli. 1990. Some properties of a phloem-limited non mechanically transmissible grapevine virus. Journal of Phytopathology 129: 151–158.

    Article  Google Scholar 

  • Castellano, M.A., G.P. Martelli, and V. Savino. 1983. Virus-like particles and ultrastructural modifications in the phloem of leafroll-affected grapevines. Vitis 22: 23–39.

    Google Scholar 

  • Castellano, M.A., and G.P. Martelli. 1984. Ultrastructure and nature of vesiculated bodies associated with isometric virus-like particles in diseased grapevines. Journal of Ultrastructure Research 89: 56–64.

    Article  Google Scholar 

  • Castellano, M.A., G.P. Martelli, V. Savino, and D. Boscia. 1985. Progress in the study of the phloem-limited isometric virus-like particles associated with leafroll-diseased grapevines. Phytopathologia Mediterranea 24: 165–169.

    Google Scholar 

  • Credi, R., and A.R. Babini. 1996. Effect of virus and virus-like infections on the growth of grapevine rootstocks. Advances in Horticultural Science 10: 95–98.

    Google Scholar 

  • Cretazzo E., C.V. Padilla, and L. Velasco, 2017. First report of grapevine red globe virus in grapevine in Spain. Plant Disease (in press) Doi:org/10.1094/PDIS-06-16-0932-PDN

  • Czotter, N., E. Szabo, J. Molnar, L. Kocisz, T. Deák, G. Bisztray, G. Tusnády, J. Burgyán, and Várallyay E. 2016. First description of grapevine Syrah virus-1 from grapevine in Hungary. Journal of Plant Pathology 97 (in press).

    Google Scholar 

  • Dreher, T.W., M.C. Edwards, A.J. Gibbs, A.-L. Haenni, R.W. Hammond, I. Jupin, R. Koenig, S. Sabanadzovic, and G.P. Martelli. 2012. Family Tymoviridae. In Virus Taxonomy. Ninth Report of the International Committee on Taxonomy of Viruses, ed. A.M.Q. King, M.J. Adams, E.B. Carstens, and E.J. Lefkowitz, 944–952. Amsterdam: Elsevier-Academic Press.

    Google Scholar 

  • Elbeaino, T., S. Sabanadzovic, M. Digiaro, N. Abou Ghanem-Sabanadzovic, A. Rowhani, P.E. Kyriakopoulou, and G.P. Martelli. 2001. Molecular detection of grapevine fleck virus-like viruses. Vitis 40: 65–68.

    CAS  Google Scholar 

  • Engel, E.A., P.A. Rivera, and P.D.T. Valenzuela. 2010. First report of grapevine Syrah virus 1 in Chilean grapevines. Plant Disease 94: 633.

    Google Scholar 

  • Engelbrecht, D.J., and G.G.F. Kasdorf. 1990. Field spread of corky bark, fleck, leafroll and Shiraz decline diseases and associated viruses in South African grapevines. Phytophylactica 22: 347–354.

    Google Scholar 

  • Fan, X.D., Y.F. Dong, Z.P. Zhang, F. Ren, G.J. Hu, Z.N. Li, and J. Zhou. 2016. First report of grapevine red globe virus (GRGV) in China. Plant Disease 100: 2340.

    Article  Google Scholar 

  • Fortusini, A., G. Scattini, S. Cinquanta, and S. Prati. 1996. Diffusione naturale del virus 1 (GLRaV-1) e del virus 3 (GLRaV-3) dell’accartocciamento fogliare e del virus della maculatura infettiva o “fleck” (GFkV) della vite. Informatore Fitopatologico 46: 39–43.

    Google Scholar 

  • Gambino, G., and I. Gribaudo. 2006. Simultaneous detection of nine grapevine viruses by multiplex reverse transcription-polymerase chain reaction with coamplification of a plant RNA as internal control. Phytopathology 96: 1223–1229.

    Article  CAS  PubMed  Google Scholar 

  • Glasa, M., L. Predajňa, and P. Kominek. 2011. Grapevine fleck virus isolates split into two distinct molecular groups. Journal of Phytopathology 159: 805–807.

    Article  Google Scholar 

  • Glasa, M., L. Predajna, K. Soltys, S. Sabanadzovic, and A. Olmos. 2015. Detection and molecular characterization of grapevine Syrah virus 1 isolates from Central Europe. Virus Genes 51: 112–121.

    Article  CAS  PubMed  Google Scholar 

  • Gugerli, P., B. Rosciglione, J.-J. Brugger, S. Bonnard, M.-E. Ramel, and F. Tremea. 1991. Further characterization of grapevine leafroll disease. Proceedings 10th meeting of ICVG, Volos, Greece: 59–60.

    Google Scholar 

  • Hammond, R.W., and P. Ramirez. 2001. Molecular characterization of the genome of maize rayado fino virus, the type member of the genus Marafivirus. Virology 282: 338–347.

    Article  CAS  PubMed  Google Scholar 

  • Hévin, M., M.M. Ottenwaelter, J.P. Doazan, and M. Rives. 1973. Investigating the transmission of marbrure and fanleaf through the seed in the grapevine. Rivista di Patologia Vegetale (Ser. IV) 9: 253–258.

    Google Scholar 

  • Hewitt, W.B. 1954. Some virus and virus-like diseases of grapevines. Bulletin California Deparment of Agriculture 43: 47–64.

    Google Scholar 

  • Hewitt, W.B., A.C. Goheen, D.J. Raski, and G.V. Gooding Jr. 1962. Studies on virus diseases of the grapevine in California. Vitis 3: 57–83.

    Google Scholar 

  • Hewitt, W.B., A.C. Goheen, L. Cory, and C. Luhn. 1972. Grapevine fleck disease, latent in many varieties, is transmitted by graft inoculation. Annalen der Physik, NHS 43–47.

    Google Scholar 

  • Immanuel, T.M., C. Delmiglio, L.I. Ward, J.O. Denton, and G.R.G. Glover. 2015. First reports of grapevine virus A, grapevine fleck virus and grapevine leafroll-associated virus 1 in the UK. Plant Disease 99: 898.

    Article  Google Scholar 

  • Jo, Y., H. Ghoi, J.K. Cho, J.-Y. Yoon, S.-K. Choi, and W.K. Cho. 2015. In silico approach to reveal viral populations in grapevine cultivar Tannat using transcriptome data. Scientific Reports 5: 15841.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kanuya, E., L.A. Clayton, R.A. Naidu, and A.V. Karasev. 2012. First report of grapevine fleck virus in Idaho grapevines. Plant Disease 96: 1705.

    Article  Google Scholar 

  • Kostadinovska, E., S. Mitrov, P.A. Bianco, P. Casati, and D. Bulgari. 2014. First report of grapevine virus A and grapevine fleck virus in the former Yugoslav Republic of Macedonia. Plant Disease 98: 1747.

    Article  Google Scholar 

  • Kumar, S., L. Singh, L. Ferretti, M. Barba, A.A. Zaidi, and V. Hallan. 2013. Evidence of grapevine leafroll-associated virus 1–3, grapevine fleck virus and grapevine virus B occurring in Himachal Pradesh, India. Indian J. Virology 24: 66–69.

    Article  Google Scholar 

  • Kuniyuki, H., and A.S. Costa. 1995. Occorrencia de mais um isolado do virus do mosaico da nervuras da videira que no causa sintomas no porta-enxerto Kober 5BB. Fitopatologia Brasileira 20: 618–622.

    Google Scholar 

  • Kyriakopoulou, P.E. 1991. Symptoms of grapevine asteroid mosaic in Greece. Proc. 10th meeting ICVG, Volos, Greece: 143–146.

    Google Scholar 

  • Kyriakopoulou, P.E., S. Tzortzakaki, and M. Tsagris. 1993. Grapevine asteroid mosaic in Greece: positive indexing results and viroids associated. Extended abstracts 11th meeting ICVG, Montreux, Switzerland: 41.

    Google Scholar 

  • Lesemann, D.E. 1977. Virus group-specific and virus-specific cytological alterations induced by members of the tymovirus group. Phytopathologische Zeitschrift 90: 315–336.

    Article  Google Scholar 

  • López-Fabuel, I., T. Wetzel, E. Bertolini, A. Bassler, E. Vidal, B.L. Torres, A. Yuste, and A. Olmos. 2013. Real-time multiplex RT-PCR for the simultaneous detection of five main grapevine viruses. Journal of Virological Methods 188: 21–24.

    Article  PubMed  Google Scholar 

  • Maliogka, V.I., G.P. Martelli, M. Fuchs, and N.I. Katis. 2015. Control of viruses infecting grapevine. Advances in Virus Research 91: 175–227.

    Article  PubMed  Google Scholar 

  • Martelli, G.P. 2014. Directory of virus and virus-like diseases of the grapevine and their agents. Journal of Plant Pathology 96 (1 Supplement): 136.

    Google Scholar 

  • Martelli, G.P., A. Rowhani, and S. Sabanadzovic. 2015. The fleck complex. In Compendium of grape diseases, ed. W.F. Wilcox, W.D. Gubler, and J.K. Uyemoto, 2nd ed., 115–117. St. Paul: APS Press.

    Google Scholar 

  • Martelli, G.P., S. Sabanadzovic, N. Abou Ghanem-Sabanadzovic, and P. Saldarelli. 2002a. Maculavirus, a new genus of plant viruses. Archives of Virology 147: 1847–1853.

    Article  CAS  PubMed  Google Scholar 

  • Martelli, G.P., S. Sabanadzovic, N. Abou Ghanem-Sabanadzovic, M.C. Edwards, and T. Dreher. 2002b. The family Tymoviridae. Archives of Virology 147: 1837–1846.

    Article  CAS  PubMed  Google Scholar 

  • Matsumoto, T., and S.T. Ohki. 1998. A possible new necrotic diseases of grapevine associated with small isometric particles and novel membrane-bound large particles. Annals of the Phytopathological Society of Japan 64: 560–564.

    Article  Google Scholar 

  • Mekuria, T.A., and R.A. Naidu. 2010. First report of grapevine virus sequences highly similar to Grapevine Syrah virus 1 from Washington vineyards. Plant Disease 94: 787.

    Article  Google Scholar 

  • Milkus, B. 1974. Mycoplasma- or chlamydia-like bodies in grape, affected by marbour. Acta Physiologica Academiae Scientiarum Hungaricae 9: 385–388.

    Google Scholar 

  • Osman, F., and A. Rowhani. 2006. Application of a spotting sample preparation technique for the detection of pathogens in woody plants by RT-PCR and real-time PCR (TaqMan). Journal of Virological Methods 133: 130–136.

    Article  CAS  PubMed  Google Scholar 

  • Osman, F., C. Leutenegger, D. Golino, and A. Rowhani. 2008. Comparison of low-density arrays, RT-PCR and real-time TaqMan RT-PCR in detection of grapevine viruses. Journal of Virological Methods 149: 292–299.

    Article  CAS  PubMed  Google Scholar 

  • Oosthuizen, K., B. Coetzee, H. Maree, and J. Burger. 2016. First report of grapevine Syrah virus-1 in South African vineyards. Plant Disease 100: 1252.

    Article  Google Scholar 

  • Ottenwaelter, M.M., M. Hévin, P. Leclair, J.P. Doazan, and M. Rives. 1973. Heat therapy eliminates the ability to transmit the causal agent of “marbrure” in several V. vinifera clones and in V. rupestris “du Lot” (St. George). Rivista Di Patologia Vegetale. (Ser. IV) 9:281–285.

    Google Scholar 

  • Pacifico, D., P. Caciagli, S. Palmano, F. Mannini, and C. Marzacchi. 2011. Quantitation of grapevine leafroll-associated virus 1, grapevine virus A, grapevine fanleaf virus and grapevine fleck virus in field-collected Vitis vinifera L. ‘Nebbiolo’ by real-time reverse transcription PCR. Journal of Virological Methods 172: 1–7.

    Article  CAS  PubMed  Google Scholar 

  • Pantaleo, V., P. Saldarelli, L. Miozzi, A. Giampetruzzi, A. Gisel, S. Moxon, T. Dalmay, G. Bisztray, and J. Burgyan. 2010. Deep sequencing analysis of viral short RNAs from an infected Pinot Noir grapevine. Virology 408: 49–56.

    Article  CAS  PubMed  Google Scholar 

  • Poojari, S., T. Lowery, M. Rott, A.-M. Schmidt, N. DeLury, J. Boulé, and J.R. Úrbez_Torres. 2016. First report and prevalence of grapevine fleck virus in grapevines (Vitis vinifera L.) in Canada. Plant Disease 100: 1028.

    Article  Google Scholar 

  • Rubino, L., M. Russo, and G.P. Martelli. 2014. Tombusvirus-induced multivesicualar bodies: Origin and role in virus-host interactions. In Plant-virus interaction. Molecular Approaches and Viral Evolution, ed. R.K. Gaur, T. Hon, and P. Sharma, 163–175. Amsterdam: Elsevier-Academic Press.

    Google Scholar 

  • Sabanadzovic, S., P. Saldarelli, and V. Savino. 1996. Molecular detection of grapevine fleck virus. Vitis 35: 137–140.

    CAS  Google Scholar 

  • Sabanadzovic, S., N. Abou Ghanem, M.A. Castellano, M. Digiaro, and G.P. Martelli. 2000. Grapevine fleck virus-like viruses in Vitis. Archives of Virology 145: 553–565.

    Article  CAS  PubMed  Google Scholar 

  • Sabanadzovic, S., N. Abou Ghanem-Sabanadzovic, P. Saldarelli, and G.P. Martelli. 2001. Complete nucleotide sequence and genomic organization of grapevine fleck virus. Journal of General Virology 82: 2009–2015.

    Google Scholar 

  • Sabanadzovic, S., N. Abou Ghanem-Sabanadzovic, and A.E. Gorbalenya. 2009. Grapevine virus Q: the first plant virus with a permuted active site of RNA-dependent RNA polymerase. Virology 394: 1–7.

    Article  CAS  PubMed  Google Scholar 

  • Schieber, O., A. Seddas, C. Belin, and B. Walter. 1997. Monoclonal antibodies for detection, serological characterization and immunopurification of grapevine fleck virus. European Journal of Plant Pathology 103: 767–774.

    Article  Google Scholar 

  • Shi, B.J., N. Habili, and R.H. Symons. 2003. Nucleotide sequence variation in a small region of grapevine fleck virus replicase provide evidence for two sequence variants of the virus. Annals of Applied Biology 142: 349–355.

    Google Scholar 

  • Spilmont, A.S., A. Ruiz, and S. Grenan. 2012. Efficiency of micrografting of shoot apices as a sensitive sanitation method against seven grapevine viruses (ArMV, GFLV, GLRaV-1, -2, -3, GFkV, GVA). Proc. 17th Cong. of ICVG, Davis, USA, 270–271.

    Google Scholar 

  • Tamura, K., G. Stecher, D. Peterson, A. Filipski, and S. Kumar. 2013. MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30: 2725–2729.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Thompson, J.R., M. Fuchs, H. McLane, F. Celebi-Toprak, K.F. Fischer, J.L. Potter, and K.L. Perry. 2014. Profiling viral infections in grapevine using a randomly primed reverse transcription-polymerase chain reaction/microarray multiplex platform. Phytopathology 104: 211–219.

    Article  CAS  PubMed  Google Scholar 

  • Triolo, E., and A. Materazzi. 1987. La maculatura infettiva della vite: Influenza di isolati diversi sull’attitudine alla propagazione vegetativa di Vitis rupestris St. George. Rech. Agronom. Suisse 26: 320–324.

    Google Scholar 

  • Vargas-Asencio, J., K. Wojciechowska, M. Baskerville, A.L. Gomez, K.L. Perry, and J.R. Thompson. 2017. The complete nucleotide sequence and genomic characterization of grapevine asteroid mosaic associated virus. Virus Research 227: 82–87.

    Article  CAS  PubMed  Google Scholar 

  • Verderevskaja, T.D., V.G. Marinesku, and E.S. Semtschik. 1983. Etiologie und diagnose der marmorierung der weinrebe. Archiv Für Phytopathologie Und Pflanzenschutz 19: 221–226.

    Article  Google Scholar 

  • Woodham, R.C., and L.R. Krake. 1983. Investigations on transmission of grapevine leafroll, yellow speckle and fleck diseases by dodder. Phytopathologische Zeitschrift 106: 193–198.

    Article  Google Scholar 

  • Xiao, H., and B. Meng. 2016. First report of grapevine asteroid mosaic-associated virus and grapevine rupestris vein feathering virus in grapevines in Canada. Plant Disease 100: 2175.

    Article  Google Scholar 

  • Yamakawa, Y. 1989. Virus reinfection of virus-free Cabernet sauvignon and Cabernet franc vines. Journal of the Japanese Society for Horticultural Science 58: 297–302.

    Article  Google Scholar 

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Acknowledgments

The authors would like to thank all scientists that contributed to the current knowledge on these viruses, providing an inspiration and a valuable source of information for the preparation of this chapter. Thanks are extended to various funding agencies that allowed studies on these viruses at the University of Bari and Mississippi State University in particular to Mississippi Agricultural and Forestry Experiment Station (MAFES) Strategic Research Initiative and NIFA Hatch Projects for financial support to SS and NA.

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  1. Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, MS, 39762, USA

    S. Sabanadzovic

  2. Institute for Genomics, Biocomputing and Biotechnology, Mississippi State University, Mississippi State, MS, 39762, USA

    N. Aboughanem-Sabanadzovic

  3. Department of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Bari, Italy

    G. P. Martelli

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Correspondence to S. Sabanadzovic .

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Editors and Affiliations

  1. Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada

    Baozhong Meng

  2. Department of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Bari, Italy

    Giovanni P. Martelli

  3. Foundation Plant Services, University of California, Davis, California, USA

    Deborah A. Golino

  4. Section of Plant Pathology and Plant-Microbe Biology, School of Integrative Plant Science, New York State Agricultural Experiment Station, Cornell University, Geneva, New York, USA

    Marc Fuchs

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Sabanadzovic, S., Aboughanem-Sabanadzovic, N., Martelli, G.P. (2017). Grapevine fleck and similar viruses. In: Meng, B., Martelli, G., Golino, D., Fuchs, M. (eds) Grapevine Viruses: Molecular Biology, Diagnostics and Management. Springer, Cham. https://doi.org/10.1007/978-3-319-57706-7_16

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