Abstract
The increasing popularity of the zebrafish (Danio rerio) as a vertebrate model organism has made it the most genetically studied vertebrate, only surpassed by the mouse. Zebrafish popularity stems from its favorable biological properties such as its high fecundity, rapid development, and (as larva) optical transparency. Recent years have seen the development of an impressive genetic toolbox for the zebrafish. While earlier geneticists had to rely on mutant strains generated by random chemical mutagenesis, zebrafish researchers have now the full complement of modern genetic tools at their fingertips. This includes efficient transposon-mediated transgenesis and CRISPR/Cas9-mediated genome editing. These recent genetic advances in combination with the optical properties of the larva enable sophisticated neural circuit analyses, unsurpassed in any other vertebrate organisms.
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Acknowledgements
We apologize to all the authors whose work we could not cite due to space limitations. We are grateful to Selin Özgut and Matthias Gesemann for comments on the manuscript. CCC was supported by the RiMED foundation; work in the authors’ laboratory is supported by the Swiss National Science Foundation (31003A_173083). Special thanks to Irene Ojeda Naharros for artwork on both figures.
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Cianciolo Cosentino, C., Neuhauss, S.C.F. (2017). Paradigms for the Quantification of Behavioral Responses in Zebrafish. In: Çelik, A., Wernet, M. (eds) Decoding Neural Circuit Structure and Function. Springer, Cham. https://doi.org/10.1007/978-3-319-57363-2_8
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