The Current State of the Neuroanatomy Toolkit in the Fruit Fly Drosophila melanogaster

  • Daryl M. GohlEmail author
  • Javier MoranteEmail author
  • Koen J.T. VenkenEmail author


The fruit fly Drosophila melanogaster is a popular workhorse model organism that has tremendously contributed to our understanding of the nervous system across eukaryotic multicellular species. Through molecular, developmental, histochemical, anatomical, and physiological experimentation, studies that incorporate fruit flies have had immediate biomedical impact relevant to neurobiology and neuropathology. D. melanogaster is one of the most well-established eukaryotic multicellular model organisms, largely due to its sophisticated and expanding in vivo targeted neurogenetic manipulations. Here, we summarize the current status of techniques for precisely targeted spatiotemporal manipulation of the fly’s nervous system, focused on the most recent developments within the field.


Binary activation system GAL4 LexA QF Reporters Logic gates Intersection Flp recombinase Cre recombinase Mitotic analysis Multistochastic labeling 



We apologize to those whose work we did not cite due to focus and space limitations. We thank Herman Dierick for critical comments on the chapter. JM is supported by the Ramon y Cajal Program (RyC-2010-07155) and grants from the Ministerio de Economia y Competitividad (SAF2012-31467 and BFU2016-76295-R), co-financed by the European Regional Development Fund (ERDF) and the “Severo Ochoa” Program for Centers of Excellence in R&D (SEV-2013-0317). KV is supported by startup funds kindly provided by Baylor College of Medicine, the Albert and Margaret Alkek Foundation, and the McNair Medical Institute, as well as grants from the March of Dimes Foundation (#1-FY14-315), the Cancer Prevention and Research Institute of Texas (R1313), and the National Institutes of Health (1R21HG006726, 1R21GM110190, 1R21OD022981, and R01GM109938).


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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.University of Minnesota Genomics CenterMinneapolisUSA
  2. 2.Instituto de Neurociencias, Consejo Superior de Investigaciones CientíficasUniversidad Miguel HernándezAlicanteSpain
  3. 3.Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Department of PharmacologyDan L. Duncan Cancer Center, Baylor College of MedicineHoustonUSA

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