Interelectrode Stretched Photoelectro-Functional DNA Nanowire
DNA/functional molecules complexes have attracted much attention for fabricating DNA-based functional nanowires. In this chapter, we describe the DNA-based functional nanowires stretched and immobilized between a pair of electrodes. First, previously reported methods for stretching of DNA as nanowires will be reviewed. Then, we mention the morphology of DNA nanowires on mica substrate without stretching and alignment treatments. Next, in order to stretch the DNA nanowires, dielectrophoretic trapping method was performed. High frequency and high electric field voltage was applied to DNA aqueous solution between a pair of comb-shaped Au electrodes. The structures of the stretched and immobilized DNA nanowires were analyzed with AFM. As the result, huge numbers of DNA nanowires was aligned and immobilized between the electrodes, forming the DNA brush-like structure. Aiming for investigation of optoelectronic properties of single molecular DNA nanowire, we have examined adequate method for obtaining singly immobilized DNA nanowire in terms of DNA concentration, applied voltage, and shape of the electrodes. As a result, we successfully fabricated almost singly stretched and immobilized DNA nanowires. Then, functionalization of the stretched DNA nanowires was subsequently carried out. As the photoelectro-functional molecule, tris(bipyridine)ruthenium(II) complex (Ru(bpy) 3 2+ ) was associated to the stretched DNA nanowires to introduce photoelectronic functionalities. The height of DNA/Ru(bpy) 3 2+ nanowires was ranging from 1.5 to 3.5 nm, which was higher than that of the native DNA. This indicated that the Ru(bpy) 3 2+ was successfully associated to stretched DNA nanowires. Fluorescent microscopy and I–V measurement were also suggested the formation of stretched and immobilized DNA/Ru(bpy) 3 2+ functional nanowires.
The authors express their appreciation to the Nippon Chemical Feed Co. Ltd for providing the salmon testes DNA sample. This work is partly supported by Grant-in-Aid for Grant-in-Aid for Scientific Research on Innovative Areas “Molecular Architechtonics” (No. 26110503) from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Ogasawara Foundation, and The Futaba Electronics Memorial Foundation.
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