Abstract
In the microbial control of pests, the entomopathogen Bacillus thuringiensis offers the best biological alternative to chemical insecticides, either alone or in combination with other methods of field control, and is also a source of genes for the genetic engineering of plants. In this work, aspects related to new targets of this bacterium are described such as: Acromyrmex spp.; Nasutitermes ehrhardt; Euschistus heros; Oryzophagus oryzae; Blatella germanica; Pyricularia grisea, Rhizoctonia solani, Fusarium oxysporum; Fusarium solani and Meloidogyne spp. Also discussed are the interactions of Bacillus thuringiensis and B. subtilis with other biological control agents: Purpureocillium lilacinus; Campoletis flavicincta; Nuclear Polyhedrosis Virus; plant extracts and essential oils from medicinal plants. Data from our research group of Microbiology and Toxicology in Agroecosystems (MToxAgro/CNPq), as well as collaborating researchers of some public and private institutions of Brazil will be presented.
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References
Araújo FF, Henning AA, Hungri M (2005) Phytohormones and antibiotics produced by Bacillus subtilis and their effects on seed pathogenic fungi and on soybean root development. World J Microbiol Biotechnol 21:1639–1645
Asaka O, Shoda M (1996) Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14. Appl Environ Microbiol 62:4081–4085
Ashoub AH, Amara MT (2010) Biocontrol activity of some bacterial genera against root-knot nematode, Meloidogyne incognita. J Am Sci 6(10):321–328
Bell HA, Down RE, Edwards JP, Gatehouse JA, Gatehouse AMR (2005) Digestive proteolytic activity in the gut and salivary glands of the predatory bug Podisus maculiventris (Heteroptera: Pentatomidae), effect of proteinase inhibitors. Eur J Entomol 102:139–145
Berlitz DL (2014) Potencial biotecnológico de Bacillus thuringiensis e Bacillus subtilis no controle biológico de nematoides. 136 f. São Leopoldo, UNISINOS. Tese (Doutorado em Biologia). Programa de Pós Graduação em Biologia, Universidade do Vale do Rio dos Sinos, São Leopoldo
Berlitz DL, Fiuza LM (2006) Bacillus thuringiensis e Melia azedarach. Aplicações e interações no controle de insetos-praga. Biotecnol, Ciênc Desenvolvimento 35:62–68
Berlitz DL, Azambuja AO, Sebben A, Oliveira JV, Fiuza LM (2012) Mortality of Oryzophagus oryzae (Costa Lima, 1936) (Coleoptera: Curculionidae) and Spodoptera frugiperda (J E Smith, 1797) (Lepidoptera: Noctuidae) larvae exposed to Bacillus thuringiensis and extracts of Melia azedarach. Braz Arch Biol Technol 55(5):725–731
Berlitz DL, Saul DA, Machado V, Santin RC, Guimarães AM, Matsumura ATS, Ribeiro BM, Fiuza LM (2013) Bacillus thuringiensis: molecular characterization, ultrastructural and nematoxicity to Meloidogyne sp. J Biopest 6(2):120–128
Berlitz DL, Rabinovitch L, Machado V, Santin RC, Guimarães AM, Matsumura ATS, Cassal M, Fiuza LM (2016) Evaluation of biocontrol of the Meloidogyne javanica with Bacillus subtilis and Purpureocillium lilacinus in greenhouse with lettuce. Int J Res Eng, IT Soc Sci 6(7):38–45
Bettiol W (1991) Controle biológico de doenças do filoplano. In: Bettiol W (ed) Controle biológico de doenças de plantas. EMBRAPA-CNPDA, Jaguariúna, p 338
Breuer M, Hoste B, De Loof A, Naqvi SNH (2003) Effect of Melia azedarach extract on the activity of NADPH-cytochrome c reductase and cholinesterase in insects. Pestic Biochem Physiol 76:99–103
Carpinella MC, Defago MT, Valladares G, Palacios SM (2003) Antifeedant and inseticide properties of a limnoid from Melia azedarach (Meliaceae) with potencial use for pest management. J Agric Food Chem 51:369–374
Castagnone-Sereno P, Danchin EG, Perfus-Barbeoch L, Abad P (2013) Diversity and evolution of root-knot nematodes, genus Meloidogyne: new insights from the genomic era. Annu Rev Phytopathol 51:203–220
Castilhos-Fortes R, Matsumura ATS, Diehl E, Fiuza LM (2002) Susceptibility of Nasutitermes ehrhardti (Isoptera: Termitidae) to Bacillus thuringiensis subspecies. Braz J Microbiol 33(3):219–222. http://dx.doi.org/10.1590/S1517-83822002000300006
Chougule NP, Bonning BC (2012) Toxins for transgenic resistance to hemipteran pests. Toxins 4:405–429
Collange B, Navarrete M, Peyre G, Mateille T, Tchamitchian M (2011) Root-knot nematode (Meloidogyne) management in vegetable crop production: the challenge of an agronomic system analysis. Crop Prot 30:1251–1262
Cunha FM, Caetano FH, Wanderley-Teixeira V, Torres JB, Teixeira AAC, Alves LC (2012) Ultra-structure and histochemistry of digestive cells of Podisus nigrispinus (Hemiptera: Pentatomidae) fed with prey reared on Bt-cotton. Mícron 43:245–250
Davies KG, Curtis RHC (2011) Cuticle surface coat of plant-parasitic nematodes. Annu Rev Phytopathol 49:135–156
Dequech STB, Silva RFP, Fiuza LM (2005) Interação entre Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae), Campoletis flavicincta (Ashmead) (Hymenoptera: Ichneumonidae) e Bacillus thuringiensis aizawai, em laboratório. Neotrop Entomol 34(6):937–944
Dequech STB, Silva RFP, Fiuza LM, Zumba RC (2007) Histopatologia de lagartas de Spodoptera frugiperda (Lep., Noctuidae) infectadas por Bacillus thuringiensis aizawai e com ovos de Campoletis flavicincta (Hym., Ichneumonidae). Ciênc Rural 37(1):273–276
Doyle EA, Lambert KN (2002) Cloning and characterization of a sophageal-gland-specific pectate lyase from the root-knot nematode Meloidogyne javanica. Mol Plant Microbe Interact 15(6):549–556
Elling AA (2013) Major emerging problems with minor Meloidogyne species. Phytopathology 103:1092–1102
El-Moneim TSA, Massoud SI (2009) The effect of endotoxin produced by Bacillus thuringiensis (Bt.) against Meloidogyne incognita. Egypt J Nat Toxins 6(1):83–93
Emprapa (2016) Disponível: https://www.embrapa.br/soja/cultivos/soja1 Consulta em 28 de novembro de 2016
Fernandes WD, Ferraz JMG, Ferracini VL, Habib MEM (1996) Deterrência alimentar e toxicidez de extratos vegetais em adultos de Anthonomus grandis Boh. (Coleoptera: Curculionidae). Anais Soc Entomológica Brasil 25:553–556
Hübner M (2004) Bioatividade de extratos vegetais e isolados de Bacillus thuringiensis nos insetos urbanos Blatella germanica (L.) e Periplaneta americana (L.) (Blattodea, Blattellidae). Dissertação: Mestrado. Universidade do vale do Rio dos Sinos, São Leopoldo. 72p
Joo SB, Kumar VJR, Ahmad RI, Kim B, Park W, Park S, Kim S, Kim S, Lim J, Park Y (2012) Bacterial mixture from greenhouse soil as a biocontrol agent against root-knot nematode, Meloidogyne incognita, on oriental melon. J Microbiol Biotechnol 22(1):114–117
Khan TA, Saxena SK (1997) Integrated management of root knot nematode Meloidogyne javanica infecting tomato using organic materials and Paecilomyces lilacinus. Bioresour Technol 61:47–250
Khan A, Williams KL, Nevalainen HKM (2004) Effects of Paecilomyces lilacinus protease and chitinase on the eggshell structures and hatching of Meloidogyne javanica juveniles. Biol Control 31:346–352
Knaak N, Fiuza LM (2005) Histopathology of Anticarsia gemmatalis Hübner (Lepidoptera; Noctuidae) treated with Nucleopolyhedrovirus and Bacillus thuringiensis serovar kurstaki. Braz J Microbiol 36(2):196–200. http://dx.doi.org/10.1590/S1517-83822005000200017
Knaak N, Rohr AA, Fiuza LM (2007) In vitro effect of Bacillus thuringiensis strains and cry proteins in phytopathogenic fungi of paddy rice-field. Braz J Microbiol 38(3):526–530. http://dx.doi.org/10.1590/S1517-83822007000300027
Knaak N, Tagliari MS, Fiuza LM (2010) Histopatologia da interação de Bacillus thuringiensis e extratos vegetais no intestino médio de Spodoptera frugiperda (Lepidoptera: Noctuidae). Arq Inst Biol 77(1):83–89
Knaak N, Wiest SLF, Soares W, Fiuza LM (2015) Natural products: insecticidal and antimicrobial activity. In: A. Mendez Vilas (ed) The battle against microbial pathogens: basic science technological advances and educational programs. Formatex, pp 328–335
Lamovsek J, Urek G, Trdan S (2013) Biological control of root-knot nematodes (Meloidogyne spp.): microbes against the pests. Acta Agric Slov 101(2):263–275
Leifert C, Li H, Chidburee S, Hampson S, Workman S, Sigee D, Epton HAS, Harbour A (1995) Antibiotic production and biocontrol activity by Bacillus subtilis CL27 and Bacillus pumilus CL45. J Appl Bacteriol 78:97–108
Lucho APR (2004) Manejo de Spodoptera frugiperda (J. E. Smith 1797) (Lepidoptera: Noctuidae) em arroz irrigado. Dissertação: Mestrado. Universidade do Vale do Rio dos Sinos – São Leopoldo. 73p
Mariano RLR, Silveira EB, Assis SMP, Maria A, Gomes A, Peixoto AR, Donato MTS (2004) Importância de bactérias promotoras de crescimento e de biocontrole de doenças de plantas para uma agricultura sustentável. Anais Acad Pernambucana Ciênc Agron 1:89–111
Mavingui P, Heulin T (1994) In vitro chitinase and antifungal activity of a soil, rhizosphere and rhizoplane population of Bacillus polymyxa. Soil Biol Biochem 26:801–803
Mitchum MG, Wang X, Wang J, Davis E (2012) Role of nematode peptides and other small molecules in plant parasitism. Annu Rev Phytopathol 50:175–195
Moens M, Perry RN (2009) Migratory plant endoparasitic nematodes: a group rich in contrasts and divergence. Annu Rev Phytopathol 47:313–332
Nitao JK, Meyer SLF, Chitwood DJ (1999) In-vitro assays of Meloidogyne incognita and Heterodera glycines for detection of nematode-antagonistic fungal compounds. J Nematol 31(2):172–183
Pal-Bais H, Fall R, Vivanco JM (2004) Biocontrol of Bacillus subtilis against infection of arabidopsis roots by Pseudomonas syringae is facilitated by biofilm formation and surfactin production. Plant Physiol 134:307–319
Pinto LMN, Azambuja AO, Diehl E, Fiuza LM (2003) Pathogenicity of Bacillus thuringiensis isolated from two species of Acromyrmex (Hymenoptera, Formicidae). Braz J Biol 63(2):301–306. http://dx.doi.org/10.1590/S1519-69842003000200015
Ravari SB, Moghaddam EM (2015) Efficacy of Bacillus thuringiensis Cry14 toxin against root knot nematode, Meloidogyne javanica. Plant Prot Sci 51(1):46–51
Rosso M, Favery B, Piotte C, Arthaud L, De Boer JM, Hussey RS, Jaap Bakker J, Baum TJ, Abad P (1999) Isolation of a cDNA encoding a b-1,4-endoglucanase in the root-knot nematode Meloidogyne incognita and expression analysis during plant parasitism. Mol Plant-Microbe Interact 12(7):585–591
Saito ML, Lucchini F (1998) Substâncias obtidas de plantas e a procura por praguicidas eficientes e seguros ao meio ambiente. EMBRAPA-CNPMA, Jaguariúna, p 46
Schünemann R (2015). Espectro inseticida da soja Bt e proteínas cry em lagartas (Anticarsia gemmatalis) e percevejos (Euschistus heros). Tese: Doutorado. Universidade do Vale do Rio dos Sinos – São Leopoldo, p 132
Sharma RD, Gomes AC (1996) Controle biológico de Meloidogyne arenaria com Pausteria penetrans. Nematol Brasileira 23(1):47–52
Siddiqui ZA, Mahmood I (1999) Role of bacteria in the management of plant parasitic nematodes: a review. Bioresour Technol 69:167–179
Spier MS et al (2013) Aspectos ecológicos de Atta sexdens piriventris Santschi (Hymenoptera: Formicidae) no município de Capinzal, Santa Catarina, Brasil. Entomobrasilis 6(1):94–96
Tian BY, Yang JK, Lian LH, Wang CY, Zhang KQ (2007) Role of neutral protease from Brevibacillus laterosporus in pathogenesis of nematode. Appl Microbiol Biotechnol 74:372–380
Todorova S, Kozhuharova L (2010) Characteristics and antimicrobial activity of Bacillus subtilis strains isolated from soil. World J Microbiol Biotechnol 26:1207–1216
Vendramin JD (2002) O controle biológico e a resistência de plantas. In: Controle Biológico no Brasil. Manole, São Paulo, pp 511–520
Vovlas N, Rapoport HF, Jiménez DRM, Castillo P (2005) Differences in feeding sites induced by root-knot nematodes, Meloidogyne spp., in chickpea. Phytopathology 95:368–375
Wei JZ, Hale C, Carta L, Platzer E, Wong C, Fang SC, Arojan RV (2003) Bacillus thuringiensis crystal proteins that target nematodes. PNAS 100(5):2760–2765
Yu Z, Xiong J, Zhou Q, Luo H, Hu S, Xia L, Sun M, Li L, Yu Z (2015) The diverse nematicidal properties and biocontrol efficacy of Bacillus thuringiensis Cry6A against the root-knot nematode Meloidogyne hapla. J Invertebr Pathol 125:73–80
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The authors thank all those who directly or indirectly participated in the elaboration of this work, contributing with data, personal communications, photographs and literature. In this context, in particular, researchers or collaborating professors and undergraduate and postgraduate students of public and private institutions in Brazil.
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Fiuza, L.M., Berlitz, D.L., de Oliveira, J.V., Knaak, N. (2017). Bacillus thuringiensis: Different Targets and Interactions. In: Fiuza, L., Polanczyk, R., Crickmore, N. (eds) Bacillus thuringiensis and Lysinibacillus sphaericus. Springer, Cham. https://doi.org/10.1007/978-3-319-56678-8_9
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