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Directional Growth for Sperm Delivery

Chapter

Abstract

Flowering plants use a polarized projection of the pollen grain called a pollen tube, to precisely deliver two sperm cells to the female gametes, which are deeply buried in the female gametophyte of the ovules for fertilization. The pollen tubes navigate a long route from the stigma, through the transmitting tract over a considerable amount of time before targeting the individual ovules. How is this precise cellular targeting achieved by the pollen tube? A lot of progress has been made toward identifying the sources of guidance cues that the pollen tube receives and the molecules that act as such guidance cues during its journey toward the ovule. In this chapter, we discuss these pollen tube guidance cues and also the latest tools in bio-imaging and microfluidics that would enable us to gain a better understanding of this process of directional growth for sperm delivery.

Keywords

Bio-imaging In vivo pollen tube growth Microfluidics 

Abbreviations

AGP

Arabinogalactan protein

Ca2+

Calcium ion

ECM

Extracellular matrix

GFP

Green fluorescent protein

LoC

Lab-on-a-chip

TPE

Two-photon excitation microscopy

Notes

Acknowledgments

This work was supported in part by the Japan Science and Technology Agency (JST), Exploratory Research for Advanced Technology (ERATO) program (JPMJER1004) and the Japan Society for the Promotion of Science KAKENHI (JP16H06465, JP16K21727, and 15F15388) to T.H. S.S is supported by JSPS Postdoctoral Fellowship for Overseas Researchers.

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© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.Institute of Transformative Bio-moleculesNagoya UniversityNagoyaJapan
  2. 2.Division of Biological Science, Graduate School of ScienceNagoya UniversityNagoyaJapan
  3. 3.JST, ERATO, Higashiyama Live-Holonics ProjectNagoya UniversityNagoyaJapan

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