Microfluidic- and Microelectromechanical System (MEMS)-Based Platforms for Experimental Analysis of Pollen Tube Growth Behavior and Quantification of Cell Mechanical Properties



Experimentation on pollen tubes has benefited greatly from recent technological developments in the fields of microfluidics and microelectromechanical systems (MEMS). Various design strategies have been developed to expose in vitro growing pollen tubes to a range of experimental assays with the aim to study their behavior and their mechanical properties. The devices allow exposing the cells to chemical gradients, microstructural features, integrated biosensors, or directional triggers, and they are compatible with Nomarski optics and fluorescence microscopy. Microfluidic technology has opened new avenues for both more efficient experimentation and large-scale phenotyping of tip-growing cells under precisely controlled, reproducible conditions. The chapter provides an overview of the different design strategies used and the type of data acquired over the past 5 years since the technique was first adopted by the pollen community.


Pollen tube growth Invasive growth Chemotropism Directed growth Tip growth Microfluidics MEMS Lab-on-a-chip 





Microelectromechanical systems


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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.Faculty of Agricultural and Environmental Sciences, Department of Plant ScienceMcGill UniversityQuébecCanada

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