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Microfluidic- and Microelectromechanical System (MEMS)-Based Platforms for Experimental Analysis of Pollen Tube Growth Behavior and Quantification of Cell Mechanical Properties

Chapter

Abstract

Experimentation on pollen tubes has benefited greatly from recent technological developments in the fields of microfluidics and microelectromechanical systems (MEMS). Various design strategies have been developed to expose in vitro growing pollen tubes to a range of experimental assays with the aim to study their behavior and their mechanical properties. The devices allow exposing the cells to chemical gradients, microstructural features, integrated biosensors, or directional triggers, and they are compatible with Nomarski optics and fluorescence microscopy. Microfluidic technology has opened new avenues for both more efficient experimentation and large-scale phenotyping of tip-growing cells under precisely controlled, reproducible conditions. The chapter provides an overview of the different design strategies used and the type of data acquired over the past 5 years since the technique was first adopted by the pollen community.

Keywords

Pollen tube growth Invasive growth Chemotropism Directed growth Tip growth Microfluidics MEMS Lab-on-a-chip 

Abbreviations

LoC

Lab-on-a-chip

MEMS

Microelectromechanical systems

References

  1. Agudelo C, Sanati Nezhad A, Ghanbari M, Packirisamy M, Geitmann A (2012) A microfluidic platform for the investigation of elongation growth in pollen tubes. J Micromech Microeng 22:115009CrossRefGoogle Scholar
  2. Agudelo C, Packirisamy M, Geitmann A (2013a) Lab-on-a-chip for studying growing pollen tubes. In: Žárský V, Cvrčková F (eds) Plant cell morphogenesis: methods and protocols, vol 1080. Methods in molecular biology. Springer, New YorkGoogle Scholar
  3. Agudelo CG, Sanati Nezhad A, Ghanbari M, Naghavi M, Packirisamy M, Geitmann A (2013b) TipChip: a modular, MEMS-based platform for experimentation and phenotyping of tip-growing cells. Plant J 73:1057–1068CrossRefPubMedGoogle Scholar
  4. Agudelo CG, Packirisamy M, Geitmann A (2016) Influence of electric fields and conductivity on pollen tube growth assessed via electrical lab-on-chip. Sci Rep 6:19812CrossRefPubMedPubMedCentralGoogle Scholar
  5. Bibikova TN, Zhigilei A, Gilroy S (1997) Root hair growth in Arabidopsis thaliana is directed by calcium and an endogenous polarity. Planta 203:495–505CrossRefPubMedGoogle Scholar
  6. Bou Daher F, Geitmann A (2011) Actin is involved in pollen tube tropism through redefining the spatial targeting of secretory vesicles. Traffic 12:1537–1551CrossRefPubMedGoogle Scholar
  7. Bou Daher F, Chebli Y, Geitmann A (2008) Optimization of conditions for germination of cold-stored Arabidopsis thaliana pollen. Plant Cell Rep 28:347–357CrossRefPubMedGoogle Scholar
  8. Brand A, Gow NAR (2009) Mechanisms of hypha orientation of fungi. Curr Opin Microbiol 12:350–357CrossRefPubMedPubMedCentralGoogle Scholar
  9. Chebli Y, Kaneda M, Zerzour R, Geitmann A (2012) The cell wall of the Arabidopsis thaliana pollen tube – spatial distribution, recycling and network formation of polysaccharides. Plant Physiol 4:1940–1955CrossRefGoogle Scholar
  10. Cheung K, Renaud P (2006) BioMEMS for medicine: on-chip cell characterization and implantable microelectrodes. Solid State Electron 50:551–557CrossRefGoogle Scholar
  11. Cheung AY, Wu H-m (2007) Structural and functional compartmentalization in pollen tubes. J Exp Bot 58:75–82CrossRefPubMedGoogle Scholar
  12. Cheung AY, Wu H-m (2008) Structural and signaling networks for the polar cell growth machinery in pollen tubes. Annu Rev Plant Biol 59:547–572CrossRefPubMedGoogle Scholar
  13. Fayant P, Girlanda O, Chebli Y, Aubin CE, Villemure I, Geitmann A (2010) Finite element model of polar growth in pollen tubes. Plant Cell 22:2579–2593CrossRefPubMedPubMedCentralGoogle Scholar
  14. Feijó JA, Malhó R, Obermeyer G (1995) Ion dynamics and its possible role during in vitro pollen germination and tube growth. Protoplasma 187:155–167CrossRefGoogle Scholar
  15. Feijó JA, Sainhas J, Holdaway-Clarke TL, Cordeiro MS, Kunkel JG, Hepler PK (2001) Cellular oscillations and the regulation of growth: the pollen tube paradigm. Bioessays 23(1):86–94CrossRefPubMedGoogle Scholar
  16. Geitmann A, Palanivelu R (2007) Fertilization requires communication: signal generation and perception during pollen tube guidance. Floric Ornament Biotechnol 1:77–89Google Scholar
  17. Geitmann A, Parre E (2004) The local cytomechanical properties of growing pollen tubes correspond to the axial distribution of structural cellular elements. Sex Plant Reprod 17:9–16CrossRefGoogle Scholar
  18. Geitmann A, McConnaughey W, Lang-Pauluzzi I, Franklin-Tong VE, Emons AMC (2004) Cytomechanical properties of Papaver pollen tubes are altered after self-incompatibility challenge. Biophys J 86:3314–3323CrossRefPubMedPubMedCentralGoogle Scholar
  19. Ghanbari M, Sanati Nezhad A, Agudelo C, Packirisamy M, Bhat R, Geitmann A (2014) Microfluidic positioning of pollen grains in lab-on-a-chip for single cell analysis. J Biosci Bioeng 117:504–511CrossRefPubMedGoogle Scholar
  20. Giouroudi I, Kosel J, Scheffer C (2008) BioMEMS in diagnostics: a review and recent developments. Recent Patents Eng 2:114–121CrossRefGoogle Scholar
  21. Gossot O, Geitmann A (2007) Pollen tube growth – coping with mechanical obstacles involves the cytoskeleton. Planta 226:405–416CrossRefPubMedGoogle Scholar
  22. Held M, Edwards C, Nicolau D (2011) Probing the growth dynamics of Neurospora crassa with microfluidic structures. Fungal Biol 115:493–505CrossRefPubMedGoogle Scholar
  23. Hepler PK, Vidali L, Cheung AY (2001) Polarized cell growth in higher plants. Annu Rev Cell Dev Biol 17:159–187CrossRefPubMedGoogle Scholar
  24. Higashiyama T, Hamamura Y (2008) Gametophytic pollen tube guidance. Sex Plant Reprod 21:17–26CrossRefGoogle Scholar
  25. Horade M, Yanagisawa N, Mizuta Y, Higashiyama T, Arata H (2014) Growth assay of individual pollen tubes arrayed by microchannel device. Microelectron Eng 118:25–28Google Scholar
  26. Jaffe L, Nuccitelli R (1977) Electrical controls of development. Annu Rev Biophys Bioeng 6:445–476CrossRefPubMedGoogle Scholar
  27. Kanaoka MM, Higashiyama T (2015) Peptide signaling in pollen tube guidance. Curr Opin Plant Biol 28:127–136CrossRefPubMedGoogle Scholar
  28. Kristen U, Kappler R (1995) The pollen tube growth test. In: O’Hare S, Atterwill CK (eds) In vitro toxicity testing protocols, Methods in molecular biology, vol 43. Humana, New York, pp 189–198CrossRefGoogle Scholar
  29. Lord EM (2003) Adhesion and guidance in compatible pollination. J Exp Bot 54:47–54CrossRefPubMedGoogle Scholar
  30. Malhó R (2006) The pollen tube: a cellular and molecular perspective, Plant cell monographs, vol 3. Springer, BerlinCrossRefGoogle Scholar
  31. Malhó R, Feijó JA, Pais MSS (1992) Effect of electrical fields and external ionic currents on pollen tube orientation. Sex Plant Reprod:57–63Google Scholar
  32. Márton M, Dresselhaus T (2010) Female gametophyte-controlled pollen tube guidance. Biochem Soc Trans 38:627–630CrossRefPubMedGoogle Scholar
  33. Messerli M, Robinson KR (1997) Tip localized Ca2+ pulses are coincident with peak pulsatile growth rates in pollen tubes of Lilium longiflorum. J Cell Sci 110:1269–1278PubMedGoogle Scholar
  34. Messerli M, Robinson KR (1998) Cytoplasmic acidification and current influx follow growth pulses of Lilium longiflorum pollen tubes. Plant J 16:87–91CrossRefGoogle Scholar
  35. Messerli MA, Robinson KR (2003) Ionic and osmotic disruption of the lily pollen tube oscillator: testing proposed models. Planta 217:147–157PubMedGoogle Scholar
  36. Money NP (2001) Biomechanics of invasive hyphal growth. In: Howard RJ, Gow NAR (eds) The Mycota: biology of the fungal cell, vol 8. Springer, New York, pp 3–17CrossRefGoogle Scholar
  37. Money NP, Davis CM, Ravishankar JP (2004) Biomechanical evidence for convergent evolution of the invasive growth process among fungi and oomycete water molds. Fungal Genet Biol 41:872–876CrossRefPubMedGoogle Scholar
  38. Nuxoll E, Siegel R (2009) BioMEMS devices for drug delivery. IEEE Eng Med Biol Mag 28:31–39CrossRefPubMedGoogle Scholar
  39. Palanivelu R, Preuss D (2000) Pollen tube targetting and axon guidance: parallels in tip growth mechanisms. Trends Cell Biol 10:517–524CrossRefPubMedGoogle Scholar
  40. Palanivelu R, Tsukamoto T (2011) Pathfinding in angiosperm reproduction: pollen tube guidance by pistils ensures successful double fertilization. WIREs Dev Biol 1:96–113Google Scholar
  41. Parre E, Geitmann A (2005a) More than a leak sealant – the physical properties of callose in pollen tubes. Plant Physiol 137:274–286CrossRefPubMedPubMedCentralGoogle Scholar
  42. Parre E, Geitmann A (2005b) Pectin and the role of the physical properties of the cell wall in pollen tube growth of Solanum chacoense. Planta 220:582–592Google Scholar
  43. Qin Y, Yang Z (2011) Rapid tip growth: insights from pollen tubes. Semin Cell Dev Biol 22:816–824CrossRefPubMedPubMedCentralGoogle Scholar
  44. Sanati Nezhad A, Geitmann A (2013) The cellular mechanics of an invasive life style. J Exp Bot 64:4709–4728CrossRefPubMedGoogle Scholar
  45. Sanati Nezhad A, Naghavi M, Packirisamy M, Bhat R, Geitmann A (2013a) Quantification of cellular penetrative forces using Lab-on-a-chip technology and finite element modeling. Proc Natl Acad Sci U S A 110:8093–8098CrossRefPubMedPubMedCentralGoogle Scholar
  46. Sanati Nezhad A, Naghavi M, Packirisamy M, Bhat R, Geitmann A (2013b) Quantification of the Young's modulus of the primary plant cell wall using bending-lab-on-chip (BLoC). Lab Chip 13:2599–2608CrossRefGoogle Scholar
  47. Sanati Nezhad A, Ghanbari M, Agudelo C, Naghavi M, Packirisamy M, Bhat R, Geitmann A (2014a) Optimization of flow assisted entrapment of pollen grains in a microfluidic platform for tip growth analysis. Biomed Microdevices 16:23–33CrossRefPubMedGoogle Scholar
  48. Sanati Nezhad A, Packirisamy M, Geitmann A (2014b) Dynamic, high precision targeting of growth modulating agents is able to trigger pollen tube growth reorientation. Plant J 80:185–195CrossRefPubMedGoogle Scholar
  49. Sato Y, Sugimoto N, Higashiyama T, Arata H (2015) Quantification of pollen tube attraction in response to guidance by female gametophyte tissue using artificial microscale pathway. J Biosci Bioeng 120:697–700Google Scholar
  50. Sawidis T, Reiss H-D (1995) Effects of heavy metals on pollen tube growth and ultrastructure. Protoplasma 185:113–122CrossRefGoogle Scholar
  51. Vogler H, Draeger C, Weber A, Felekis D, Eichenberger C, Routier-Kierzkowska A-L, Boisson-Dernier A, Ringli C, Nelson BJ, Smith RS, Grossniklaus U (2013) The pollen tube: a soft shell with a hard core. Plant J 73:617–627CrossRefPubMedGoogle Scholar
  52. Yetisen A, Jiang L, Cooper J, Qin Y, Palanivelu R, Zohar Y (2011) A microsystem-based assay for studying pollen tube guidance in plant reproduction. J Micromech Microeng 21:e054018CrossRefGoogle Scholar
  53. Yu H, Meyvantsson I, Shkel I, Beebe D (2005) Diffusion dependent cell behavior in microenvironments. Lab Chip 5:1089–1095CrossRefPubMedGoogle Scholar
  54. Zerzour R, Kroeger JH, Geitmann A (2009) Polar growth in pollen tubes is associated with spatially confined dynamic changes in cell mechanical properties. Dev Biol 334:437–446CrossRefPubMedGoogle Scholar

Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.Faculty of Agricultural and Environmental Sciences, Department of Plant ScienceMcGill UniversityQuébecCanada

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