The Pollen Membrane Proteome



The male gametophyte (or pollen) is a highly specialized organ essential for sexual reproduction of higher plants. Their reduced complexity constitutes them as an ideal experimental system for analyses of biological processes maintaining tip growth. Rapid advances in proteomic technologies and a vast choice of metabolic labelling and label-free quantitation protocols as well as the availability of full genome sequences allow comprehensive analyses of various pollen proteomes. Pollen membrane proteome consists of integral and membrane-associated proteins involved in regulation of many cellular functions. In this chapter, novel insights into identification of membrane proteins by proteome analysis and how their dynamic subcellular localization contributes to the initiation of pollen grain germination and maintenance of tube growth are discussed.


Mass spectrometry Membrane proteins Protein-protein interactions Proteomics 



Two-dimensional polyacrylamide gel electrophoresis


Two-dimensional difference in gel electrophoresis


Autoinhibited-type Ca2+ ATPase


Bimolecular fluorescence complementation




Coat protein complex


Endoplasmic reticulum-type Ca2+ ATPase


Endoplasmic reticulum


Electrospray ionization quadrupole time-of-flight tandem mass spectrometry


Green fluorescent protein


Isoelectric focusing


Liquid chromatography coupled with multistage accurate mass spectrometry


Matrix-assisted laser desorption/ionization time-of-flight mass spectrometer


Mating-based split-ubiquitin system


Isoelectric point


Plasma membrane intrinsic proteins


Plasma membrane


Soluble N-ethylmaleimide-sensitive factor attachment protein receptor


Trans-Golgi network


Voltage-dependent anion channel


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© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.Molecular Plant Biophysics and Biochemistry, Department of Molecular BiologyUniversity of SalzburgSalzburgAustria

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