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Pollen Tip Growth pp 293–318Cite as

The Pollen Membrane Proteome

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Abstract

The male gametophyte (or pollen) is a highly specialized organ essential for sexual reproduction of higher plants. Their reduced complexity constitutes them as an ideal experimental system for analyses of biological processes maintaining tip growth. Rapid advances in proteomic technologies and a vast choice of metabolic labelling and label-free quantitation protocols as well as the availability of full genome sequences allow comprehensive analyses of various pollen proteomes. Pollen membrane proteome consists of integral and membrane-associated proteins involved in regulation of many cellular functions. In this chapter, novel insights into identification of membrane proteins by proteome analysis and how their dynamic subcellular localization contributes to the initiation of pollen grain germination and maintenance of tube growth are discussed.

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Abbreviations

2D-PAGE:

Two-dimensional polyacrylamide gel electrophoresis

2D DIGE:

Two-dimensional difference in gel electrophoresis

ACA:

Autoinhibited-type Ca2+ ATPase

BiFC:

Bimolecular fluorescence complementation

CaM:

Calmodulin

COP:

Coat protein complex

ECA:

Endoplasmic reticulum-type Ca2+ ATPase

ER:

Endoplasmic reticulum

ESI Q-TOF MS/MS:

Electrospray ionization quadrupole time-of-flight tandem mass spectrometry

GFP:

Green fluorescent protein

IEF:

Isoelectric focusing

LC-MSn :

Liquid chromatography coupled with multistage accurate mass spectrometry

MALDI-TOF MS:

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometer

mbSUS:

Mating-based split-ubiquitin system

pI :

Isoelectric point

PIP:

Plasma membrane intrinsic proteins

PM:

Plasma membrane

SNARE:

Soluble N-ethylmaleimide-sensitive factor attachment protein receptor

TGN:

Trans-Golgi network

VDAC:

Voltage-dependent anion channel

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  1. Molecular Plant Biophysics and Biochemistry, Department of Molecular Biology, University of Salzburg, Billrothstr. 11, 5020, Salzburg, Austria

    Heidi Pertl-Obermeyer

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  1. Department of Molecular Biology, University of Salzburg, Salzburg, Austria

    Gerhard Obermeyer

  2. Cell Biology Molecular Genetics, University of Maryland, College Park, Maryland, USA

    José Feijó

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Pertl-Obermeyer, H. (2017). The Pollen Membrane Proteome. In: Obermeyer, G., Feijó, J. (eds) Pollen Tip Growth. Springer, Cham. https://doi.org/10.1007/978-3-319-56645-0_11

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