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Isolation and Molecular Characterization of Circulating Tumor Cells pp 67–81Cite as

Aptamer-Based Methods for Detection of Circulating Tumor Cells and Their Potential for Personalized Diagnostics

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 994))

Abstract

Cancer diagnostics and treatment monitoring rely on sensing and counting of rare cells such as cancer circulating tumor cells (CTCs) in blood. Many analytical techniques have been developed to reliably detect and quantify CTCs using unique physical shape and size of tumor cells and/or distinctive patterns of cell surface biomarkers. Main problems of CTC bioanalysis are in the small number of cells that are present in the circulation and heterogeneity of CTCs. In this chapter, we describe recent progress towards the selection and application of synthetic DNA or RNA aptamers to capture and detect CTCs in blood. Antibody-based approaches for cell isolation and purification are limited because of an antibody’s negative effect on cell viability and purity. Aptamers transform cell isolation technology, because they bind and release cells on-demand. The unique feature of anti-CTC aptamers is that the aptamers are selected for cell surface biomarkers in their native state, and conformation without previous knowledge of their biomarkers. Once aptamers are produced, they can be used to identify CTC biomarkers using mass spectrometry. The biomarkers and corresponding aptamers can be exploited to improve cancer diagnostics and therapies .

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References

  • Allard WJ, Matera J, Miller MC et al (2004) Tumor cells circulate in the peripheral blood of all major carcinomas but not in healthy subjects or patients with nonmalignant diseases. Clin Cancer Res 10(20):6897–6904

    Article  PubMed  Google Scholar 

  • Alunni-Fabbroni M, Sandri M (2010) Circulating tumour cells in clinical practice: methods of detection and possible characterization. Methods 50(4):289–297

    Article  CAS  PubMed  Google Scholar 

  • Chen G, Huang C, Wu H et al (2014) Isolating and concentrating rare cancerous cells in large sample volumes of blood by using dielectrophoresis and stepping electric fields. Biochip J 8(2):67–74

    Article  CAS  Google Scholar 

  • Dickey D, Giangrande P (2016) Oligonucleotide aptamers: a next-generation technology for the capture and detection of circulating tumor cells. Methods 97:94–103

    Article  CAS  PubMed  Google Scholar 

  • Hatanaka H, Yasukawa T, Mizutani F (2011) Detection of surface antigens on living cells through incorporation of immunorecognition into the distinct positioning of cells with positive and negative dielectrophoresis. Anal Chem 83(18):7207–7212

    Article  CAS  PubMed  Google Scholar 

  • Jacob K, Sollier C, Jobodo N (2007) Circulating tumor cells: detection, molecular profiling and future prospects. Expert Rev Proteomics 4(6):741–756

    Article  CAS  PubMed  Google Scholar 

  • Jayasena S (1999) Aptamers: an emerging class of molecules that rival antibodies in diagnostics. Clin Chem 45(9):1628–1650

    CAS  PubMed  Google Scholar 

  • Joosse S, Pantel K (2013) Biologic challenges in the detection of circulating tumor cells. Cancer Res 73(1):8–11

    Article  CAS  PubMed  Google Scholar 

  • Lampignano R, Schneck H, Neumann M et al (2016) Detection of EpCAM-negative circulating tumor cells in metastatic breast cancer patients by using VyCAP filters technology. Oncol Res Treat 39:48–48

    Google Scholar 

  • Li S, Chen N, Zhang Z et al (2013) Endonuclease-responsive aptamer-functionalized hydrogel coating for sequential catch and release of cancer cells. Biomaterials 34(2):460–469

    Article  PubMed  Google Scholar 

  • Liu G, Mao X, Phillips J et al (2009) Aptamer-nanoparticle strip biosensor for sensitive detection of cancer cells. Anal Chem 81(24):10013–10018

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Maremanda N, Roy K, Kanwar R et al (2015) Quick chip assay using locked nucleic acid modified epithelial cell adhesion molecule and nucleolin aptamers for the capture of circulating tumor cells. Biomicrofluidics 9(5):054110

    Article  PubMed  PubMed Central  Google Scholar 

  • Martin J, Phillips J, Parekh P et al (2011) Capturing cancer cells using aptamer-immobilized square capillary channels. Mol BioSyst 7(5):1720–1727

    Article  CAS  PubMed  Google Scholar 

  • Nellore B, Kanchanapally R, Pramanik A et al (2015) Aptamer-conjugated graphene oxide membranes for highly efficient capture and accurate identification of multiple types of circulating tumor cells. Bioconjug Chem 26(2):235–242

    Article  Google Scholar 

  • Punnoose E, Atwal S, Liu W et al (2012) Evaluation of circulating tumor cells and circulating tumor DNA in non-small cell lung cancer: association with clinical endpoints in a phase II clinical trial of pertuzumab and erlotinib. Clin Cancer Res 18(8):2391–2401

    Article  CAS  PubMed  Google Scholar 

  • Qu L, Xu J, Tan X et al (2014) Dual-aptamer modification generates a unique Interface for highly sensitive and specific electrochemical detection of tumor cells. ACS Appl Mater Interfaces 6(10):7309–7315

    Article  CAS  PubMed  Google Scholar 

  • Rusconi C, Scardino E, Layzer J et al (2002) RNA aptamers as reversible antagonists of coagulation factor IXa. Nature 419(6902):90–94

    Article  CAS  PubMed  Google Scholar 

  • Sefah K, Meng L, Lopez-Colon D et al (2010) DNA aptamers as molecular probes for colorectal cancer study. PLoS One 5(12):e14269

    Article  PubMed  PubMed Central  Google Scholar 

  • Shangguan D, Li Y, Tang Z et al (2006) Aptamers evolved from live cells as effective molecular probes for cancer study. Proc Natl Acad Sci U S A 103(32):11838–11843

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shen Q, Xu L, Zhao L et al (2013) Specific capture and release of circulating tumor cells using aptamer-modified nanosubstrates. Adv Mater 25(16):2368–2373

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sheng W, Chen T, Katnath R et al (2012) Aptamer-enabled efficient isolation of cancer cells from whole blood using a microfluidic device. Anal Chem 84(9):4199–4206

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sheng W, Chen T, Tan W et al (2013) Multivalent DNA Nanospheres for enhanced capture of cancer cells in microfluidic devices. ACS Nano 7(8):7067–7076

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sheng W, Ogunwobi O, Chen T et al (2014) Capture, release and culture of circulating tumor cells from pancreatic cancer patients using an enhanced mixing chip. Lab Chip 14(1):89–98

    Article  CAS  PubMed  Google Scholar 

  • Sun N, Wang J, Ji L et al (2015) A cellular compatible chitosan nanoparticle surface for isolation and in situ culture of rare number CTCs. Small 11(40):5444–5451

    Article  CAS  PubMed  Google Scholar 

  • Tang Z, Shangguan D, Wang K et al (2007) Selection of aptamers for molecular recognition and characterization of cancer cells. Anal Chem 79(13):4900–4907

    Article  CAS  PubMed  Google Scholar 

  • Wan Y, Tan J, Asghar W et al (2011) Velocity effect on aptamer-based circulating tumor cell isolation in microfluidic devices. J Phys Chem B 115(47):13891–13896

    Article  CAS  PubMed  Google Scholar 

  • Wan Y, Liu Y, Allen P et al (2012) Capture, isolation and release of cancer cells with aptamer-functionalized glass bead array. Lab Chip 12(22):4693–4701

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang L, Zheng Q, Zhang Q et al (2012) Detection of single tumor cell resistance with aptamer biochip. Oncol Lett 4(5):935–940

    CAS  PubMed  PubMed Central  Google Scholar 

  • Wehbe M, Labib M, Muharemagic D et al (2015) Switchable aptamers for biosensing and bioseparation of viruses (SwAps-V). Biosens Bioelectron 67:280–286

    Article  CAS  PubMed  Google Scholar 

  • Zamay G, Kolovskaya O, Zamay T et al (2015) Aptamers selected to postoperative lung adenocarcinoma detect circulating tumor cells in human blood. Mol Ther 23(9):1486–1496

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zeng Z, Tung C, Zu Y (2014) A cancer cell-activatable aptamer-reporter system for one-step assay of circulating tumor cells. Mol Ther Nucl Acid 3:e184

    Article  CAS  Google Scholar 

  • Zhang J, Li S, Liu F et al (2015) SELEX aptamer used as a probe to detect circulating tumor cells in peripheral blood of pancreatic cancer patients. PLoS One 10(3):e0121920

    Article  PubMed  PubMed Central  Google Scholar 

  • Zhao L, Tang C, Xu L et al (2016) Enhanced and differential capture of circulating tumor cells from lung cancer patients by microfluidic assays using aptamer cocktail. Small 12(8):1072–1081

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zheng F, Cheng Y, Wang J et al (2014) Aptamer-functionalized barcode particles for the capture and detection of multiple types of circulating tumor cells. Adv Mater 26(43):7333–7338

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This work was supported by Ministry of Education and Science of Russian Federation Federal Target Program # 14.604.21.0105.

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Authors and Affiliations

  1. Laboratory for Biomolecular and Medical Technologies, Krasnoyarsk State Medical University named after Professor V. F. Voyno-Yasenetsky, Krasnoyarsk, Russia

    Anna S. Zamay, Galina S. Zamay, Olga S. Kolovskaya & Tatiana N. Zamay

  2. Federal Research Center, KSC Siberian branch of Russian Academy of Science, Krasnoyarsk, Russia

    Anna S. Zamay, Galina S. Zamay & Olga S. Kolovskaya

  3. Department of Chemistry and Biomolecular Sciences, University of Ottawa, 10 Marie Curie, Ottawa, ON, K1N 6N5, Canada

    Maxim V. Berezovski

Authors
  1. Anna S. Zamay
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  2. Galina S. Zamay
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  3. Olga S. Kolovskaya
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  4. Tatiana N. Zamay
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  5. Maxim V. Berezovski
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Corresponding authors

Correspondence to Anna S. Zamay or Maxim V. Berezovski .

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Editors and Affiliations

  1. University of California San Francisco, San Francisco, California, USA

    Mark Jesus M. Magbanua

  2. University of California San Francisco, San Francisco, California, USA

    John W. Park

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Zamay, A.S., Zamay, G.S., Kolovskaya, O.S., Zamay, T.N., Berezovski, M.V. (2017). Aptamer-Based Methods for Detection of Circulating Tumor Cells and Their Potential for Personalized Diagnostics. In: Magbanua, M., Park, J. (eds) Isolation and Molecular Characterization of Circulating Tumor Cells. Advances in Experimental Medicine and Biology, vol 994. Springer, Cham. https://doi.org/10.1007/978-3-319-55947-6_3

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