Abstract
Thus far we did not pay too much attention to the fluorescence lifetime (Sect. 2.1). As the lifetime of the excited state is in the range of a few nanoseconds, and therefore not easy to capture by measurement devices, for a long time it was not a standard parameter that would be used to characterize fluorescence phenomena. This has changed in the last 20 years, and measurements based on fluorescence lifetime are found more frequently in the literature. In this chapter we discuss simple lifetime measurements and how we can benefit from these relationships in everyday microscopy.
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Further Reading
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Borlinghaus, R.T. (2017). Separation in the Time Domain. In: The White Confocal. Springer, Cham. https://doi.org/10.1007/978-3-319-55562-1_9
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DOI: https://doi.org/10.1007/978-3-319-55562-1_9
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