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Brain Tissue PO2 Measurement During Normoxia and Hypoxia Using Two-Photon Phosphorescence Lifetime Microscopy

  • Kui Xu
  • David A. Boas
  • Sava Sakadžić
  • Joseph C. LaMannaEmail author
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 977)

Abstract

Key to the understanding of the principles of physiological and structural acclimatization to changes in the balance between energy supply (represented by substrate and oxygen delivery, and mitochondrial oxidative phosphorylation) and energy demand (initiated by neuronal activity) is to determine the controlling variables, how they are sensed and the mechanisms initiated to maintain the balance. The mammalian brain depends completely on continuous delivery of oxygen to maintain its function. We hypothesized that tissue oxygen is the primary sensed variable. In this study two-photon phosphorescence lifetime microscopy (2PLM) was used to determine and define the tissue oxygen tension field within the cerebral cortex of mice to a cortical depth of between 200–250 μm under normoxia and acute hypoxia (FiO2 = 0.10). High-resolution images can provide quantitative distributions of oxygen and intercapillary oxygen gradients. The data are best appreciated by quantifying the distribution histogram that can then be used for analysis. For example, in the brain cortex of a mouse, at a depth of 200 μm, tissue oxygen tension was mapped and the distribution histogram was compared under normoxic and mild hypoxic conditions. This powerful method can provide for the first time a description of the delivery and availability of brain oxygen in vivo.

Keywords

Oxygen partial pressure 2PLM Tissue oxygen tension Distribution histogram Mouse 

Notes

Acknowledgments

This study was supported by the NIH grants R01 NS38632, R24 NS092986, R01 NS091230, R01 NS055104, and R01 EB021018.

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Copyright information

© Springer International Publishing AG 2017

Authors and Affiliations

  • Kui Xu
    • 1
  • David A. Boas
    • 2
  • Sava Sakadžić
    • 2
  • Joseph C. LaManna
    • 1
    Email author
  1. 1.Department of Physiology and BiophysicsCase Western Reserve University, School of MedicineClevelandUSA
  2. 2.Optics Division, Athinoula A. Martinos Center for Biomedical Imaging, Department of RadiologyMassachusetts General Hospital and Harvard Medical SchoolCharlestownUSA

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