Abstract
This work presents a thin film device, combining, on the same glass substrate, photosensors and long-pass interferential filter to achieve a compact and efficient sensor for biomolecule detection. The photosensors are amorphous silicon stacked structures, while the interferential filter is fabricated alternating layers of silicon dioxide and titanium dioxide, directly grown over the photosensors. The system has been optimized to effectively detect the natural fluorescence of Ochratoxin A, a highly toxic mycotoxin present in different food commodities. In particular, the long-pass interferential filter has been designed to reject the wavelengths arising from the excitation source (centered at 330 nm) thus transmitting the OTA emission spectrum (centered at 470 nm). Experimental results show that the filter strongly reduces the photosensors quantum efficiency below 420 nm, while keeps it nearly constant at higher wavelength.
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References
F. Costantini, C. Sberna, G. Petrucci, C. Manetti, G. de Cesare, A. Nascetti, D. Caputo, Lab-on-chip system combining a microfluidic-ELISA with an array of amorphous silicon photosensors for the detection of celiac disease epitopes. Sens. Bio-Sens. Res. 6, 51–58 (2015)
http://www.sigmaaldrich.com/catalog/product/sigma/se120014?lang=it®ion=IT
http://www.biooscientific.com/Mycotoxin-test-kits/MaxSignal-Ochratoxin-A-ELISA-Test-Kit
H. Meng, Z. Wang, S. De Saeger, Y. Wang, K. Wen, S. Zhang, J. Shen, Determination of Ochratoxin A in cereals and feeds by ultra-performance liquid chromatography coupled to tandem mass spectrometry with immunoaffinity column clean-up. Food Anal. Methods 7(4), 854–864 (2014)
A. Manz, N. Graber, H.M. Widmer, Miniaturized total chemical-analysis systems—a novel concept for chemical sensing. Sens. Actuat. B-Chem. 1(1–6), 244–248 (1990)
L. Gervais, N. de Rooij, E. Delamarche, Microfluidic chips for point-of-care immunodiagnostics. Adv. Mater. 23(24), H151–H176 (2011)
D. Caputo, G. de Cesare, M. Nardini, A. Nascetti, R. Scipinotti, Monitoring of tempera-ture distribution in a thin film heater by an array of a-Si: H temperature sensors. IEEE Sens. J. 12(5), 1209–1213 (2012)
D. Caputo, M. Ceccarelli, G. de Cesare, A. Nascetti, R. Scipinotti, Lab-on-Glass system for DNA analysis using thin and thick film technologies. Proceedings of material research symposium, vol. 1191, OO06-01 (2009)
K. Lewotsky, Microfluidics streamlines laboratory operations. SPIE Newsroom (2010). doi:10.1117/2.2201012.01
F. Berthold, M. Hennecke, J. Wulf, Instrumentation for chemi-luminescence and bioluminescence, in Chemiluminescence and Bioluminescence—Past, Present and Future, ed. by A. Roda (RSC, Cambridge, 2011), pp. 113–139
D. Caputo, G. de Cesare, A. Nascetti, R. Negri, Spectral tuned amorphous silicon p-i-n for DNA detection. J. Non-Cryst. Solids 352(9-20 SPEC. ISS.), 2004–2006 (2006)
A.C. Pimentel, A.T. Pereira, V. Chu, D.M.F. Prazeres, J.P. Conde, Detection of chemiluminescence using an amorphous silicon photodiode. IEEE Sens. J. 7(3–4), 415–416 (2007)
C.R. Vistas, S.S. Soares, R.M.M. Rodrigues, V. Chu, J.P. Conde, G.N.M. Ferreira, An amorphous silicon photodiode microfluidic chip to detect nanomolar quantities of HIV-1 virion infectivity factor. Analyst 139(15), 3709–3713 (2014)
F. Costantini, A. Nascetti, R. Scipinotti, F. Domenici, S. Sennato, L. Gazza, F. Bordi, N. Pogna, C. Manetti, D. Caputo, G. de Cesare, On-chip detection of multiple serum antibodies against epitopes of celiac disease by an array of amorphous silicon sensors. RSC Adv. 4(4), 2073–2080 (2014)
M. Mirasoli, A. Nascetti, D. Caputo, M. Zangheri, R. Scipinotti, L. Cevenini, G. de Cesare, A. Roda, Multiwell cartridge with integrated array of amorphous silicon photosensors for chemiluminescence detection: development, characterization and comparison with cooled-CCD luminograph. Anal. Bioanal. Chem. 406(23), 5645–5656 (2014)
D. Caputo, G. de Cesare, C. Fanelli, A. Nascetti, A. Ricelli, R. Scipinotti, Innovative detection system of ochratoxin a by thin film photodiodes. Sensors 7(7), 1317–1322 (2007)
H.A. Clark, S.M. Snedeker, Ochratoxin A: its cancer risk and potential for exposure. J. Toxicol. Environ. Health. Part B, Crit. Rev. 9(3), 265–296 (2006)
N. Palma, S. Cinelli, O. Sapora, S.H. Wilson, E. Dogliotti, Ochratoxin A-induced mutagenesis in mammalian cells is consistent with the production of oxidative stress. Chem. Res. Toxicol. 20(7), 1031–1037 (2007)
L. Al-Anati, E. Petzinger, Immunotoxic activity of ochratoxin A. J. Vet. Pharmacol. Ther. 29(2), 79–90 (2006)
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Caputo, D., Parisi, E., Nascetti, A., Tucci, M., de Cesare, G. (2018). Integration of Amorphous Silicon Photosensors with Thin Film Interferential Filter for Biomolecule Detection. In: Andò, B., Baldini, F., Di Natale, C., Marrazza, G., Siciliano, P. (eds) Sensors. CNS 2016. Lecture Notes in Electrical Engineering, vol 431. Springer, Cham. https://doi.org/10.1007/978-3-319-55077-0_16
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