Abstract
Multianalyte biosensor patch based on polymeric microneedles (MNs) have been fabricated by photolithography of poly (ethylene glycol) diacrylate (PEGDA) and have been dynamically characterized by means of quartz crystal microbalance and electrochemical measurements. The resulting flexible device acts as multianalyte working electrodes detecting glucose and lactic acid into interstitial liquid by means of redox reaction with glucose oxidase (GOx) and lactose oxidase (LOx) enzymes. Sensitivities of the order of nA mM−1 in the mM range are revealed in both case, after five minutes of swelling time and ten second of interaction.
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References
J. Wang, Electrochemical glucose biosensors. Chem. Rev. 108(2), 814–825 (2008)
N. Nikolaus, B. Strehlitz, Amperometric lactate biosensors and their application in (sports) medicine, for life quality and wellbeing. Microchim. Acta 160(1–2), 15–55 (2008)
L. Ventrelli, L.M. Strambini, G. Barillaro, Microneedles for transdermal biosensing: current picture and future direction. Adv. Healthc. Mater. (2015). doi:10.1002/adhm.201500450
A. Caliò et al., “Polymeric microneedles based enzymatic electrodes for electrochemical biosensing of glucose and lactic acid.” Sens. Actuators B: Chem. 236, 343–349 (2016)
P. Dardano et al., “Optically monitored drug delivery patch based on porous silicon and polymer microneedles.” Biomed Opt. Express. 7(5), 1645–1655 (2016)
Gabriela Valdés-Ramíreza et al., Multiplexed and switchable release of distinct fluids from microneedle platforms via conducting polymer nanoactuators for potential drug delivery. Sens. Actuators B: Chem. 161(1), 1018–1024 (2012)
J. Yan et al., Immobilizing enzymes onto electrode arrays by hydrogel photolithography to fabricate multi-analyte electrochemical biosensors. ACS Appl. Mater. Interfaces 2(3), 748–755 (2010)
P. Dardano et al., A photolithographic approach to polymeric microneedles array fabrication. Materials, 8(12), 8661–8673 (2015). doi:10.3390/ma8125484
C. Nicolini et al., High-sensitivity biosensor based on LB technology and on nanogravimetry. Sens. Actuators B Chem. 24, 121–128 (1995)
R. Spera et al., NAPPA based nanogravimetric biosensor: preliminary characterization. Sens. Actuators B 182, 682–688 (2013)
T. Bezerra Correia, V. Bavastrello, C. Nicolini, Calcium oxide matrices and carbon dioxide sensors. Sensors (Basel) 12, 5896–5905 (2012)
N.Y. Shim, D.A. Bernards, D.J. Macaya, J.A. DeFranco, M. Nikolou, R.M. Owens, G.G. Malliaras, All-plastic electrochemical transistor for glucose sensing using a ferrocene mediator. Sensors 9(12), 9896–9902 (2009)
N. Thomas, I. Lähdesmäki, B.A. Parviz, A contact lens with an integrated lactate sensor. Sens. Actuators B: Chem. 162(1), 128–134 (2012)
L. Strambini, A. Longo, S. Scarano, T. Prescimone, I. Palchetti, M. Minunni, D. Giannessi, G. Barillaro, Biosens. Bioelectron. 66, 162 (2015)
V. Di Palma, M.F. Bevilacqua, A. Di Matteo, P. Dardano, Microneedle array device for biosensor applications. US Application Patent 14558779
Acknowledgments
This work was partially supported by Italian National Operative Program FUZI (00029_3148467) of IMAST Scarl. The Authors gratefully acknowledge Prof. A. Lombardi of University of Naples Federico II and her team, for electrochemical characterizations.
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Dardano, P., Caliò, A., Di Palma, V., Bevilacqua, M.F., Di Matteo, A., De Stefano, L. (2018). Multianalyte Biosensor Patch Based on Polymeric Microneedles. In: Andò, B., Baldini, F., Di Natale, C., Marrazza, G., Siciliano, P. (eds) Sensors. CNS 2016. Lecture Notes in Electrical Engineering, vol 431. Springer, Cham. https://doi.org/10.1007/978-3-319-55077-0_10
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DOI: https://doi.org/10.1007/978-3-319-55077-0_10
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