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Development of In Vitro Maturation for Human Oocytes pp 337–350Cite as

Laboratory Aspect of IVM Treatment

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Abstract

IVM treatment requires more intensive work in laboratory in order to produce viable embryos for transfer. The source of immature will affect directly the success rate of IVM treatment. It is important to identify the immature oocytes under dissecting microscope during immature oocyte retrieval, because the shape of cumulus–oocyte complexes (COCs) with immature oocyte is quite different from mature oocyte. Immature COCs can be cultured in different maturation media supplemented with hormones and growth factors for maturation. In vitro-matured oocytes can be inseminated by IVF or ICSI method, and the fertilized zygotes can be cultured to blastocyst stage depending on the number of immature oocytes retrieved. There is no real evidence to prove that IVM of oocytes causes more aneuploidy embryos and epigenetic or imprinting disorders.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, McGill University, Montreal, QC, Canada

    Ri-Cheng Chian MSc, PhD

  2. Center for Reproductive Medicine, The 10th People’s Hospital of Tongji University, No. 301 Yanchang Road, Shanghai, 200072, P. R. of China

    Zhi-Yong Yang MD

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  1. Ri-Cheng Chian MSc, PhD
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  2. Zhi-Yong Yang MD
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Correspondence to Ri-Cheng Chian MSc, PhD .

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Editors and Affiliations

  1. McGill University, Longueuil, Québec, Canada

    Ri-Cheng Chian

  2. St. George's Hospital, London, United Kingdom

    Geeta Nargund

  3. Stanford University, Palo Alto, California, USA

    Jack Y. J. Huang

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Chian, RC., Yang, ZY. (2017). Laboratory Aspect of IVM Treatment. In: Chian, RC., Nargund, G., Huang, J. (eds) Development of In Vitro Maturation for Human Oocytes. Springer, Cham. https://doi.org/10.1007/978-3-319-53454-1_25

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