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IVM as Clinical Treatment: Overview

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Development of In Vitro Maturation for Human Oocytes

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Abstract

The oocyte is a unique cell in woman body, not only in its special structure and function but also in undergoing meiosis. Meiotic progression in the oocyte is defined as oocyte maturation from re-initiation of the first meiotic division from germinal vesicle (GV) stage to metaphase-II (M-II) stage. In vivo meiotic resumption of oocyte is initiated in response to the pre-ovulatory surge of luteinizing hormone (LH). LH surge triggers oocyte maturation from GV stage to M-II stage. Practically for infertility treatment with in vitro fertilization (IVF) technology, the patients are given human chorionic gonadotropin (hCG) to trigger the completion of oocyte meiosis in the follicles in order to retrieve M-II oocytes after 36 h of hCG injection. Without hCG injection in IVF treatment, most oocytes retrieved will be at immature GV stage. In this Part IV, we will discuss how was IVM developed as clinical treatment, and the current methodology of IVM treatment, and the outcome of IVM treatment as well as the further development of IVM treatment.

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References

  1. Pincus G, Enzmann EV. The comparative behavior of mammalian eggs in vivo and in vitro: I. The activation of ovarian eggs. J Exp Med. 1935;62:665–75.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Pincus G, Saunders B. Unfertilized human tubal ova. Anat Rec. 1937;69:163–9.

    Article  Google Scholar 

  3. Pincus G, Saunders B. The comparative behaviours of mammalian eggs in vitro and in vivo. VI. The maturation of human ovarian ova. Anat Red 1939; 75: 537–45.

    Google Scholar 

  4. Rock J, Menkin MF. In vitro fertilization and cleavage of human ovarian eggs. Science. 1944;100:105–7.

    Article  CAS  PubMed  Google Scholar 

  5. Menkin MF, Rock J. In vitro fertilization and cleavage of human ovarian eggs. Am J Obstet Gynecol. 1948;55:440–52.

    Article  CAS  PubMed  Google Scholar 

  6. Edwards RG. Maturation in vitro of mouse, sheep, cow, pig, rhesus monkey and human ovarian oocytes. Nature. 1965;208:349–51.

    Article  CAS  PubMed  Google Scholar 

  7. Edwards RG. Maturation in vitro of human ovarian oocytes. Lancet. 1965;286:926–9.

    Article  Google Scholar 

  8. Kennedy JF, Donahue RP. Human oocytes: maturation in chemically defined media. Science. 1969;164:1292–3.

    Article  CAS  PubMed  Google Scholar 

  9. Kennedy JF, Donahue RP. Binucleate human oocytes from large follicles. Lancet. 1969;7598:754–5.

    Article  Google Scholar 

  10. Edwards RG, Bavister BD, Steptoe PC. Early stages of fertilization in vitro of human oocytes matured in vitro. Nature. 1969;221:632–5.

    Article  CAS  PubMed  Google Scholar 

  11. Edwards RG, Steptoe PC, Purdy JM. Fertilization and cleavage in vitro of preovulator human oocytes. Nature. 1970;227:1307–9.

    Article  CAS  PubMed  Google Scholar 

  12. Steptoe PC, Edwards RG. Birth after the reimplantation of a human embryo. Lancet. 1978;312:366.

    Article  Google Scholar 

  13. Cha KY, Koo JJ, Ko JJ, Choi DH, Han SY, Yoon TK. Pregnancy after in vitro fertilization of human follicular oocytes collected from nonstimulated cycles, their culture in vitro and their transfer in a donor oocyte program. Fertil Steril. 1991;55:109–13.

    Article  CAS  PubMed  Google Scholar 

  14. Trounson A, Wood C, Kausche A. In vitro maturation and the fertilization and developmental competence of oocytes recovered from untreated polycystic ovarian patients. Fertil Steril. 1994;62:353–62.

    Article  CAS  PubMed  Google Scholar 

  15. Trounson A, Anderiesz C, Jones GM, Kausche A, Lolatgis N, Wood C. Oocyte maturation. Hum Reprod. 1998;13(Suppl 3):52–62.

    Article  CAS  PubMed  Google Scholar 

  16. Chian RC, Buckett WM, Too LL, Tan SL. Pregnancies resulting from in vitro matured oocytes retrieved from patients with polycystic ovary syndrome after priming with human chorionic gonadotropin. Fertil Steril. 1999;72:639–42.

    Article  CAS  PubMed  Google Scholar 

  17. Chian RC, Gulekli B, Buckett WM, Tan SL. Priming with human chorionic gonadotropin before retrieval of immature oocytes in women with infertility due to the polycystic ovary syndrome. N Engl J Med. 1999;341:1624–6.

    Article  CAS  PubMed  Google Scholar 

  18. Chian RC, Buckett WM, Tulandi T, Tan SL. Prospective randomized study of human chorionic gonadotrophin priming before immature oocyte retrieval from unstimulated women with polycystic ovarian syndrome. Hum Reprod. 2000;15:165–70.

    Article  CAS  PubMed  Google Scholar 

  19. De Vos M, Ortega-Hrepich C, Albuz FK, Guzman L, Polyzos NP, Smitz J, Devroey P. Clinical outcome of non-hCG-primed oocyte in vitro maturation treatment in patients with polycystic ovaries and polycystic ovary syndrome. Fertil Steril. 2011;96:860–4.

    Article  PubMed  Google Scholar 

  20. Chian RC, Lim JH, Tan SL. State of the art in in-vitro oocyte maturation. Curr Opin Obstet Gynecol. 2004;16:211–9.

    Article  PubMed  Google Scholar 

  21. Chian RC, Xu CL, Huang JY, Ata B. Obstetric outcomes and congenital abnormalities in infants conceived with oocytes matured in vitro. Facts Views Vis Obgyn. 2014;6:15–8.

    PubMed  PubMed Central  Google Scholar 

  22. Barnes FL, Crombie A, Gardner DK, Kausche A, Lacham-Kaplan O, Suikkari AM, Tiglias J, Wood C, Trounson AO. Blastocyst development and birth after in-vitro maturation of human primary oocytes, intracytoplasmic sperm injection and assisted hatching. Hum Reprod. 1995;10:3243–7.

    Article  CAS  PubMed  Google Scholar 

  23. Barnes FL, Kausche A, Tiglias J, Wood C, Wilton L, Trounson A. Production of embryos from in vitro-matured primary human oocytes. Fertil Steril. 1996;65:1151–6.

    Article  CAS  PubMed  Google Scholar 

  24. Cha KY, Han SY, Chung HM, Choi DH, Lim JM, Lee WS, Ko JJ, Yoon TK. Pregnancies and deliveries after in vitro maturation culture followed by in vitro fertilization and embryo transfer without stimulation in women with polycystic ovary syndrome. Fertil Steril. 2000;73:978–83.

    Article  CAS  PubMed  Google Scholar 

  25. Wynn P, Picton HM, Krapez JA, Rutherford AJ, Balen AH, Gosden RG. Pretreatment with follicle stimulating hormone promotes the numbers of human oocytes reaching metaphase II by in-vitro maturation. Hum Reprod. 1998;13:3132–8.

    Article  CAS  PubMed  Google Scholar 

  26. Liu J, Katz E, Garcia JE, Compton G, Baramki TA. Successful in vitro maturation of human oocytes not exposed to human chorionic gonadotropin during ovulation induction, resulting in pregnancy. Fertil Steril. 1997;67:566–8.

    Article  CAS  PubMed  Google Scholar 

  27. Mikkelsen AL, Smith SD, Lindenberg S. In-vitro maturation of human oocytes from regularly menstruating women may be successful without follicle stimulating hormone priming. Hum Reprod. 1999;14:1847–51.

    Article  CAS  PubMed  Google Scholar 

  28. Suikkari AM, Tulppala M, Tuuri T, Hovatta O, Barnes F. Luteal phase start of low-dose FSH of follicles results in an efficient recovery, maturation and fertilization of immature human oocytes. Hum Reprod. 2000;15:747–51.

    Article  CAS  PubMed  Google Scholar 

  29. Mikkelsen AL, Lindenberg S. Benefit of FSH priming of women with PCOS to the in vitro maturation procedure and the outcome: a randomized prospective study. Reproduction. 2001;122:587–92.

    Article  CAS  PubMed  Google Scholar 

  30. Mikkelsen AL, Host E, Blaabjerg J, Lindenberg S. Time interval between FSH priming and aspiration of immature human oocytes for in-vitro maturation: a prospective randomized study. Reprod Biomed Online. 2003;6:416–20.

    Article  CAS  PubMed  Google Scholar 

  31. Hreinsson J, Rosenlund B, Friden B, Levkov L, Ek I, Suikkari AM, Hovatta O, Fridstrom M. Recombinant LH is equally effective as recombinant hCG in promoting oocyte maturation in a clinical in-vitro maturation programme: a randomized study. Hum Reprod. 2003;18:2131–6.

    Article  CAS  PubMed  Google Scholar 

  32. Lin YH, Hwang JL, Huang LW, Mu SC, Seow KM, Chung J, Hsieh BC, Huang SC, Chen CY, Chen PH. Combination of FSH priming and hCG priming for in-vitro maturation of human oocytes. Hum Reprod. 2003;18:1632–6.

    Article  CAS  PubMed  Google Scholar 

  33. Veeck LL, Wortham JJ, Witmyer J, Sandow BA, Acosta AA, Garcia JE, Jones GS, Jones HJ. Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization. Fertil Steril. 1983;39:594–602.

    Article  CAS  PubMed  Google Scholar 

  34. Nagy ZP, Cecile J, Liu J, Loccufier A, Devroey P, Van Steirteghem A. Pregnancy and birth after intracytoplasmic sperm injection of in vitro matured germinal-vesicle stage oocytes: case report. Fertil Steril. 1996;65:1047–50.

    Article  CAS  PubMed  Google Scholar 

  35. Edirisinghe WR, Junk SM, Matson PL, Yovich JL. Birth from cryopreserved embryos following in-vitro maturation of oocytes and intracytoplasmic sperm injection. Hum Reprod. 1997;12:1056–8.

    Article  CAS  PubMed  Google Scholar 

  36. Check ML, Brittingham D, Check JH, Choe JK. Pregnancy following transfer of cryopreserved-thawed embryos that had been a result of fertilization of all in vitro matured metaphase or germinal stage oocytes. Case report. Clin Exp Obstet Gynecol. 2001;28:69–70.

    CAS  PubMed  Google Scholar 

  37. Chian RC, Park SE, Park EH. Molecular and structural characteristics between immature human oocytes retrieved from stimulated and unstimulated ovaries. In: Gomel V, Leung PCK, editors. In vitro fertilization and assisted reproduction. Monduzzi Editore, 1997; 315–19.

    Google Scholar 

  38. Cha KY, Chian RC. Maturation in vitro of immature human oocytes for clinical use. Hum Reprod Update. 1998;4:103–20.

    Article  CAS  PubMed  Google Scholar 

  39. Hwang JL, Lin YH, Tsai YL, Hsieh BC, Huang LW, Huang SC, Hsieh ML. Oocyte donation using immature oocytes from a normal ovulatory woman. Acta Obstet Gynecol Scand. 2002;81:274–5.

    Article  PubMed  Google Scholar 

  40. Nagle F, Sator MO, Juza J, Huber JC. Successful pregnancy resulting from in-vitro matured oocytes retrieved at laparoscopic surgery in a patient with polycystic ovary syndrome: case report. Hum Reprod. 2002;17:373–4.

    Article  Google Scholar 

  41. Son WY, Yoon SH, Lee SW, Ko Y, Yoon HG, Lim JH. Blastocyst development and pregnancies after IVF of mature oocytes retrieved from unstimulated patients with PCOS after in-vivo hCG priming. Hum Reprod. 2002;17:134–6.

    Article  PubMed  Google Scholar 

  42. Carmina E, Lobo RA. Polycystic ovary syndrome (PCOS): arguably the most common endocrinopathy is associated with significant morbidity in women. J Clin Endocrinol Metab. 1999;84:1897–9.

    Article  CAS  PubMed  Google Scholar 

  43. Beerendonk CC, van Dop PA, Braat DD, Merkus JM. Ovarian hyperstimulation syndrome: facts and fallacies. Obstet Gynecol Surv. 1998;53:439–49.

    Article  CAS  PubMed  Google Scholar 

  44. Smits G, Olatunbosun O, Delbaere A, Pierson R, Vassart G, Costagliola S. Ovarian hyperstimulation syndrome due to a mutation in the follicle-stimulating hormone receptor. N Engl J Med. 2003;349:760–6.

    Article  CAS  PubMed  Google Scholar 

  45. Hillier SG. Current concepts of the roles of follicle stimulating hormone and luteinizing hormone in folliculogenesis. Hum Reprod. 1994;9:188–91.

    Article  CAS  PubMed  Google Scholar 

  46. Baerwald AR, Adams GP, Pierson RA. A new model for ovarian follicular development during the human menstrual cycle. Fertil Steril. 2003;80:116–22.

    Article  PubMed  Google Scholar 

  47. Baerwald AR, Adams GP, Pierson RA. Characterization of ovarian follicular wave dynamics in women. Biol Reprod. 2003;69:1023–31.

    Article  CAS  PubMed  Google Scholar 

  48. Smith LC, Olivera-Angel M, Groome NP, Bhatia B, Price CA. Oocyte quality in small antral follicles in the presence or absence of a large dominant follicle in cattle. J Reprod Fertil. 1996;106:193–9.

    Article  CAS  PubMed  Google Scholar 

  49. Chian RC, Chung JT, Downey BR, Tan SL. Maturational and developmental competence of immature oocytes retrieved from bovine ovaries at different phases of folliculogenesis. Reprod Biomed Online. 2002;4:127–32.

    Article  CAS  PubMed  Google Scholar 

  50. Chian RC, Buckett WM, Abdul-Jalil AK, Son WY, Sylvestre C, Rao D, Tan SL. Natural cycle in vitro fertilization combined with in vitro maturation of immature oocytes is an alternative approach in infertility treatment. Fertil Steril. 2004;84:1675–8.

    Article  Google Scholar 

  51. Lim JH, Yang SH, Chian RC. New alternative to infertility treatment for women without ovarian stimulation. Reprod Biomed Online. 2007;14:547–9.

    Article  PubMed  Google Scholar 

  52. Lim JH, Yang SH, Xu Y, Yoon SH, Chian RC. Selection of patients for natural cycle in vitro fertilization combined with in vitro maturation of immature oocytes. Fertil Steril. 2009;91:1050–5.

    Article  PubMed  Google Scholar 

  53. Janssens RM1, Lambalk CB, Vermeiden JP, Schats R, Schoemaker J. In-vitro fertilization in a spontaneous cycle: easy, cheap and realistic. Hum Reprod 2000; 15: 314–18.

    Google Scholar 

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, McGill University, Montreal, Quebec, Canada

    Ri-Cheng Chian MSc, PhD

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  1. Ri-Cheng Chian MSc, PhD
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Correspondence to Ri-Cheng Chian MSc, PhD .

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Editors and Affiliations

  1. McGill University, Longueuil, Québec, Canada

    Ri-Cheng Chian

  2. St. George's Hospital, London, United Kingdom

    Geeta Nargund

  3. Stanford University, Palo Alto, California, USA

    Jack Y. J. Huang

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Chian, RC. (2017). IVM as Clinical Treatment: Overview. In: Chian, RC., Nargund, G., Huang, J. (eds) Development of In Vitro Maturation for Human Oocytes. Springer, Cham. https://doi.org/10.1007/978-3-319-53454-1_22

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  • DOI: https://doi.org/10.1007/978-3-319-53454-1_22

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-53452-7

  • Online ISBN: 978-3-319-53454-1

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