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Herpesvirus Capsid Assembly and DNA Packaging

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Cell Biology of Herpes Viruses

Part of the book series: Advances in Anatomy, Embryology and Cell Biology ((ADVSANAT,volume 223))

Abstract

Herpes simplex virus type I (HSV-1) is the causative agent of several pathologies ranging in severity from the common cold sore to life-threatening encephalitic infection. During productive lytic infection, over 80 viral proteins are expressed in a highly regulated manner, resulting in the replication of viral genomes and assembly of progeny virions. The virion of all herpesviruses consists of an external membrane envelope, a proteinaceous layer called the tegument, and an icosahedral capsid containing the double-stranded linear DNA genome. The capsid shell of HSV-1 is built from four structural proteins: a major capsid protein, VP5, which forms the capsomers (hexons and pentons), the triplex consisting of VP19C and VP23 found between the capsomers, and VP26 which binds to VP5 on hexons but not pentons. In addition, the dodecameric pUL6 portal complex occupies 1 of the 12 capsid vertices, and the capsid vertex specific component (CVSC), a heterotrimer complex of pUL17, pUL25, and pUL36, binds specifically to the triplexes adjacent to each penton. The capsid is assembled in the nucleus where the viral genome is packaged into newly assembled closed capsid shells. Cleavage and packaging of replicated, concatemeric viral DNA requires the seven viral proteins encoded by the UL6, UL15, UL17, UL25, UL28, UL32, and UL33 genes. Considerable advances have been made in understanding the structure of the herpesvirus capsid and the function of several of the DNA packaging proteins by applying biochemical, genetic, and structural techniques. This review is a summary of recent advances with respect to the structure of the HSV-1 virion capsid and what is known about the function of the seven packaging proteins and their interactions with each other and with the capsid shell.

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Acknowledgements

The research in the laboratories of F.L.H. and J.F.C. was supported by National Institutes of Health awards (AI060836).

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  1. Department of Biological Sciences, Duquesne University, Pittsburgh, PA, 15282, USA

    Jason D. Heming & Fred L. Homa

  2. Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA, 15261, USA

    James F. Conway

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  1. Institut für Virologie, Freie Universität Berlin, Berlin, Germany

    Klaus Osterrieder

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Heming, J.D., Conway, J.F., Homa, F.L. (2017). Herpesvirus Capsid Assembly and DNA Packaging. In: Osterrieder, K. (eds) Cell Biology of Herpes Viruses. Advances in Anatomy, Embryology and Cell Biology, vol 223. Springer, Cham. https://doi.org/10.1007/978-3-319-53168-7_6

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