Mass Cultivation of Mycorrhiza-Like Fungus Piriformospora indica (Serendipita indica) by Batch in Bioreactor
Piriformospora indica (Serendipita indica) is an axenically cultivable root endophytic fungus which exerts plant growth promoting effects on its host plants. To enable commercial production of its chlamydospores, Serendipita indica was cultivated in a 10 l batch bioreactor condition in such that they result in maximum biomass during growth phase and in maximum chlamydospore yield during subsequent sporulation phase. An enhancement of 100% in overall biomass productivity (0.18 g l−1 h−1) and reduction of about 70% in the time (60 h) required to achieve the maximum spore yield (9.25 × 109 spores ml−1) was achieved in comparison to the original batch culture grown in Hill and Kaefer medium. The high chlamydospore yield obtained promises to be economical for commercial production of P. indica.
KeywordsSpecific Growth Rate Batch Culture Batch Fermentation Fungal Culture Mass Cultivation
Ajit Varma is thankful to Department of Science and Technology and Department of Biotechnology for partial financial funding and to DST-FIST for providing confocal microscope facility.
- Ahuja MR (1986) Aspen. In: Evans DA, Sharp WR, Ammirato PJ (eds) Handbook of plant cell culture 4, techniques and applications. Macmillan, New York, pp 626–651Google Scholar
- Bagde US, Prasad R, Varma A (2010) Mass cultivation of Piriformospora indica in new Brunswick fermenter and its formulation as biofertilizer. Asian J Microbiol Biotechnol Environ Sci 12:911–916Google Scholar
- Gallowey LD, Burgess R (1962) Applied mycology and bacteriology, 3rd edn. Leonard Hill, London, pp 54–57Google Scholar
- Kaefer E (1977) Meiotic and mitotic recombination in Aspergillus and its chromosomal aberrations. Adv Genet 19:33–131Google Scholar
- Pham GH, Kumari R, Singh A, Malla A, Prasad R, Sachdev M, Kaldorf M, Buscot F, Oelmüller R, Hampp R, Saxena AK, K-H Rexer, Kost G, Varma A (2004) Axenic culture of symbiotic fungus Piriformospora indica. Plant surface microbiology, pp 593–613Google Scholar
- Prasad R, Pham HG, Kumari R, Singh A, Yadav V, Sachdev M, Garg AP, Peskan T, Hehl S, Sherameti I, Oelmüller R, Varma A (2005) Sebacinaceae: culturable Mycorrhiza-like endosymbiotic fungi and their interaction with nontransformed and transformed roots. In: Declerck S, Strullu D-G, Fortin A (eds) In vitro culture of mycorrhizas. Soil biology, vol 4. Springer, BerlinGoogle Scholar
- Singh A, Sharma J, Rexer KH, Varma A (2000) Plant productivity determinants beyond minerals, water and light. Piriformospora indica – a revolutionary plant growth-promoting fungus. Curr Sci 79:101–106Google Scholar
- Varma A, Singh A, Sahay NS, Sharma J, Roy A, Kumari M, Rana D, Thakran S, Deka D, Bharti K, Franken P, Hurek T, Blechert O, Rexer KH, Kost G, Hahn A, Hock B, Maier W, Walter M, Strack D, Kranner I (2001) Piriformospora indica: a cultivable mycorrhiza-like endosymbiotic fungus. In: Hock B (ed) Mycota IX. Springer, Berlin, pp 123–150Google Scholar