Abstract
One of the well-known functions of the bacterial cytoskeleton is plasmid segregation. Type II plasmid segregation systems, among the best characterized with respect to the mechanism of action, possess an actin-like cytomotive filament as the motor component. This chapter describes the essential components of the plasmid segregation machinery and their mechanism of action, concentrating on the actin-like protein family of the bacterial cytoskeleton. The structures of the actin-like filaments depend on their nucleotide state and these in turn contribute to the dynamics of the filaments. The components that link the filaments to the plasmid DNA also regulate filament dynamics. The modulation of the dynamics facilitates the cytomotive filament to function as a mitotic spindle with a minimal number of components.
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Acknowledgements
The work in the lab is supported by INSPIRE Faculty Research Grant, Department of Science and Technology (DST), India, and Innovative Young Biotechnologist Award, Department of Biotechnology, India. PG and SH acknowledge INSPIRE (DST) and IISER-Pune fellowships.
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Gayathri, P., Harne, S. (2017). Structure and Dynamics of Actin-Like Cytomotive Filaments in Plasmid Segregation. In: Löwe, J., Amos, L. (eds) Prokaryotic Cytoskeletons. Subcellular Biochemistry, vol 84. Springer, Cham. https://doi.org/10.1007/978-3-319-53047-5_10
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