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Blood Sampling and Preparation Procedures for Proteomic Biomarker Studies of Psychiatric Disorders

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Proteomic Methods in Neuropsychiatric Research

Part of the book series: Advances in Experimental Medicine and Biology ((PMISB,volume 974))

Abstract

A major challenge in proteomic biomarker discovery and validation for psychiatric diseases is the inherent biological complexity underlying these conditions. There are also many technical issues which hinder this process such as the lack of standardization in sampling, processing and storage of bio-samples in preclinical and clinical settings. This chapter describes a reproducible procedure for sampling blood serum and plasma that is specifically designed for maximizing data quality output in two-dimensional gel electrophoresis, multiplex immunoassay and mass spectrometry profiling studies.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Tissue Biology, Laboratory of Neuroproteomics, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato 255 F/01, Cidade Universitária ZeferinoVaz, 13083-862, Campinas, Brazil

    Paul C. Guest

  2. Department of Chemical Engineering and Biotechnology, University of Cambridge, CB2 3RA, Cambridge, UK

    Hassan Rahmoune

Authors
  1. Paul C. Guest
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  2. Hassan Rahmoune
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Correspondence to Hassan Rahmoune .

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Editors and Affiliations

  1. Department of Biochemistry and Tissue Biology, University of Campinas (UNICAMP), Campinas, Brazil

    Paul C. Guest

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Guest, P.C., Rahmoune, H. (2017). Blood Sampling and Preparation Procedures for Proteomic Biomarker Studies of Psychiatric Disorders. In: Guest, P. (eds) Proteomic Methods in Neuropsychiatric Research. Advances in Experimental Medicine and Biology(), vol 974. Springer, Cham. https://doi.org/10.1007/978-3-319-52479-5_9

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