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SILAC Mass Spectrometry Profiling: A Psychiatric Disorder Perspective

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Proteomic Methods in Neuropsychiatric Research

Part of the book series: Advances in Experimental Medicine and Biology ((PMISB,volume 974))

Abstract

Stable isotope labelling by amino acids in cell culture (SILAC) is a technique that allows proteomic profiling of cells. In this chapter we describe a protocol for the identification and quantification of newly synthesised proteins. The methodology can be applied to any cultured cell system with relevance to schizophrenia, affective disorders and autism spectrum conditions including those addressing responses to pharmacological stimuli.

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Acknowledgements

DDG was the recipient of a FAPESP post-doctoral fellowship (BEPE-PD 2014/20380-5) and is funded by the BHF (PG/14/20/30769). JdAF was the recipient of a FAPESP doctoral fellowship (BEPE-DR 2014/17012-4); TPO was a recipient of a Visiting Scientist CAPES Science Without Borders fellowship (BEX 11766-13-1). SEO is funded by the Medical Research Council (MC_UU_12012/4).

Author information

Authors and Affiliations

  1. University of Cambridge Metabolic Research Laboratories and MRC Metabolic Diseases Unit, Wellcome Trust-MRC Institute of Metabolic Science, Addenbrooke’s Hospital, Cambridge, UK

    Daniella Duque-Guimarães, Thomas Prates Ong, Juliana de Almeida-Faria & Susan E. Ozanne

  2. Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil

    Daniella Duque-Guimarães

  3. Food Research Center (FoRC) and Faculty of Pharmaceutical Sciences, University of São Paulo, São Paulo, Brazil

    Thomas Prates Ong

  4. Department of Pharmacology, Faculty of Medical Sciences, University of Campinas (UNICAMP), Campinas, Brazil

    Juliana de Almeida-Faria

  5. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Rua Monteiro Lobato 255 F/01, Cidade Universitária ZeferinoVaz, 13083-862, Campinas, Brazil

    Paul C. Guest

Authors
  1. Daniella Duque-Guimarães
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  2. Thomas Prates Ong
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  3. Juliana de Almeida-Faria
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  4. Paul C. Guest
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  5. Susan E. Ozanne
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Corresponding author

Correspondence to Susan E. Ozanne .

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Editors and Affiliations

  1. Department of Biochemistry and Tissue Biology, University of Campinas (UNICAMP), Campinas, Brazil

    Paul C. Guest

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Duque-Guimarães, D., Ong, T.P., de Almeida-Faria, J., Guest, P.C., Ozanne, S.E. (2017). SILAC Mass Spectrometry Profiling: A Psychiatric Disorder Perspective. In: Guest, P. (eds) Proteomic Methods in Neuropsychiatric Research. Advances in Experimental Medicine and Biology(), vol 974. Springer, Cham. https://doi.org/10.1007/978-3-319-52479-5_27

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