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Application of iTRAQ Shotgun Proteomics for Measurement of Brain Proteins in Studies of Psychiatric Disorders

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Book cover Proteomic Methods in Neuropsychiatric Research

Part of the book series: Advances in Experimental Medicine and Biology ((PMISB,volume 974))

Abstract

Shotgun proteomics has been used successfully for more than two decades for measurement of proteins from diverse biological systems. This has led to new insights and identification of potential biomarkers and drug targets for numerous medical conditions. The advent of mass-labeling approaches such as isobaric tagging for relative and absolute quantitation (iTRAQ) has allowed multiplexing of samples to improve the accuracy and throughput of experiments and the reduction of costs. Here, we describe a detailed iTRAQ mass spectrometry protocol for simultaneous analysis of four proteomes from postmortem brain tissue extracts. In addition, a post-labeling procedure for peptide fractionation is presented.

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Acknowledgments

National Council of Technological and Scientific Development (CNPq) (Grant number: 477325/2013-0) and Rio de Janeiro State Foundation for Research Support (FAPERJ) (Grant number: E-26/202.801/2015).

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Authors and Affiliations

  1. Laboratory of Protein Chemistry – Proteomics Unit, Chemistry Institute, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

    Erika Velásquez Núñez, Gilberto Barbosa Domont & Fábio César Sousa Nogueira

  2. Department of Biochemistry and Tissue Biology, Laboratory of Neuroproteomics, Institute of Biology, University of Campinas, CidadeUniversitáriaZeferinoVaz, Campinas, Brazil

    Paul C. Guest & Daniel Martins-de-Souza

Authors
  1. Erika Velásquez Núñez
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  2. Paul C. Guest
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  3. Daniel Martins-de-Souza
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  4. Gilberto Barbosa Domont
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  5. Fábio César Sousa Nogueira
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Corresponding author

Correspondence to Fábio César Sousa Nogueira .

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Editors and Affiliations

  1. Department of Biochemistry and Tissue Biology, University of Campinas (UNICAMP), Campinas, Brazil

    Paul C. Guest

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Núñez, E.V., Guest, P.C., Martins-de-Souza, D., Domont, G.B., Nogueira, F.C.S. (2017). Application of iTRAQ Shotgun Proteomics for Measurement of Brain Proteins in Studies of Psychiatric Disorders. In: Guest, P. (eds) Proteomic Methods in Neuropsychiatric Research. Advances in Experimental Medicine and Biology(), vol 974. Springer, Cham. https://doi.org/10.1007/978-3-319-52479-5_18

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