Abstract
Multiplex immunoassays allow for the rapid profiling of biomarker proteins in biological fluids, using less sample and labour than in single immunoassays. This chapter details the methods to develop and manufacture a 5-plex immunoassay for the Luminex® platform. Although assay development is not included here, the same methods can be used to covalently couple antibodies to the Luminex beads and to label antibodies for the screening of sandwich pairs, if needed. An example will be given for the analysis of five hormones (glucagon-like peptide 1, growth hormone, insulin, leptin and thyroid-stimulating hormone) in serum samples from schizophrenia patients and controls.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ryan MC, Thakore JH (2002) Physical consequences of schizophrenia and its treatment: the metabolic syndrome. Life Sci 71:239–257
Meyer JM, Davis VG, Goff DC, McEvoy JP, Nasrallah HA, Davis SM et al (2008) Change in metabolic syndrome parameters with antipsychotic treatment in the CATIE Schizophrenia Trial: prospective data from phase 1. Schizophr Res 101:273–286
Spelman LM, Walsh PI, Sharifi N, Collins P, Thakore JH (2007) Impaired glucose tolerance in first-episode drug-naïve patients with schizophrenia. Diabet Med 24:481–485
Fulton RJ, McDade RL, Smith PL, Kienker LJ, Kettman JR Jr (1997) Advanced multiplexed analysis with the FlowMetrix system. Clin Chem 43:1749–1756
Schwarz E, Guest PC, Rahmoune H, Harris LW, Wang L, Leweke FM et al (2012) Identification of a biological signature for schizophrenia in serum. Mol Psychiatry 17:494–502
Guest PC, Schwarz E, Krishnamurthy D, Harris LW, Leweke FM, Rothermundt M et al (2011) Altered levels of circulating insulin and other neuroendocrine hormones associated with the onset of schizophrenia. Psychoneuroendocrinology 36:1092–1096
Stephen L (2016) Multiplex immunoassay profiling. Methods Mol Biol 1546:169–176
Todd DJ, Knowlton N, Amato M, Frank MB, Schur PH, Izmailova ES et al (2011) Erroneous augmentation of multiplex assay measurements in patients with rheumatoid arthritis due to heterophilic binding by serum rheumatoid factor. Arthritis Rheum 63:894–903
Fraser S, Soderstrom C (2014) Due diligence in the characterization of matrix effects in a total IL-13 Singulex™ method. Bioanalysis 6:1123–1129
Krika LJ (1999) Human anti-animal antibody interferences in immunological assays. Clin Chem 45:942–956
Jani D, Allinson J, Berisha F, Cowan KJ, Devanarayan V, Gleason C et al (2016) Recommendations for use and fit-for-purpose validation of biomarker multiplex ligand binding assays in drug development. AAPS J 18:1–14
Bastarache JA, Koyama Y, Wickersham NE, Ware LB (2014) Validation of a multiplex electrochemiluminescent immunoassay platform in human and mouse samples. J Immunol Methods 408:13–23
Tighe PJ, Ryder RR, Todd I, Fairclough LC (2015) ELISA in the multiplex era: potentials and pitfalls. Proteomics Clin Appl 9:1862–8354
Tighe P, Negm O, Todd I, Fairclough L (2013) Utility, reliability and reproducibility of immunoassay multiplex kits. Methods 61:23–29
Ellington AA, Kullo IJ, Bailey RK, Klee GG (2010) Antibody-based protein multiplex platforms: technical and operational challenges. Clin Chem 56:186–193
Spindel S, Sapsford KE (2014) Evaluation of optical detection platforms for multiplexed detection of proteins and the need for point-of-care biosensors for clinical use sensors. Sensors (Basel) 14:22313–22341
Marquette CA, Corgier BP, Blum LJ (2012) Recent advances in multiplex immunoassays. Bioanalysis 4:927–936
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer International Publishing AG
About this chapter
Cite this chapter
Stephen, L., Schwarz, E., Guest, P.C. (2017). Multiplex Immunoassay Profiling of Serum in Psychiatric Disorders. In: Guest, P. (eds) Proteomic Methods in Neuropsychiatric Research. Advances in Experimental Medicine and Biology(), vol 974. Springer, Cham. https://doi.org/10.1007/978-3-319-52479-5_10
Download citation
DOI: https://doi.org/10.1007/978-3-319-52479-5_10
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-52478-8
Online ISBN: 978-3-319-52479-5
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)