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Investigating Chromatin Organisation Using Single Molecule Localisation Microscopy

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Chromatin Architecture

Part of the book series: Springer Theses ((Springer Theses))

Abstract

In this chapter, I discuss the technical details of single molecule localisation microscopy (SMLM) to investigate spatial and temporal organisation of DNA. The DNA is hierarchically folded at multiple levels to become more compacted and functionally organise itself inside of the nucleus. This spatial arrangement in turn affects the functionality of DNA.

My intention is not to replace one set of general rules by another such set: my intention is, rather, to convince the reader that all methodologies, even the most obvious ones, have their limits.

Paul Karl Feyerabend

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Notes

  1. 1.

    Parts of this chapter, including figures and captions, are based on the following publications: Szczurek et al. 2014; Żurek-Biesiada et al. 2015, 2016; Hagmann et al. 2014; Best et al. 2014.

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  1. Heidelberg University, Heidelberg, Germany

    Kirti Prakash

  2. Institute of Molecular Biology (IMB), Mainz, Germany

    Kirti Prakash

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Prakash, K. (2017). Investigating Chromatin Organisation Using Single Molecule Localisation Microscopy. In: Chromatin Architecture. Springer Theses. Springer, Cham. https://doi.org/10.1007/978-3-319-52183-1_2

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