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Detection of Phytoplasmas Associated to Grapevine Yellows Diseases in Research and Diagnostics

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Grapevine Yellows Diseases and Their Phytoplasma Agents

Part of the book series: SpringerBriefs in Agriculture ((BRIEFSAGRO))

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Abstract

Research into grapevine yellows diseases and their control relies on detection and identification of the phytoplasmas associated with them. Detection methods for phytoplasmas can be divided into four main categories: biological tests, microscopy techniques, and immunological and molecular approaches. The suitability of each of these methods for different studies is discussed in this chapter, along with their advantages and disadvantages. Among these methods, PCR-based assays in particular are routinely used in diagnostic laboratories because of their high sensitivity and potential to be automated for high-throughput testing. Recently, isothermal amplification methods have been developed for rapid on-site phytoplasma diagnostics, such as loop-mediated isothermal amplification assays. The development of any diagnostic assay requires thorough validation to ensure its sensitivity, specificity, repeatability, and reproducibility and that the assay is fit for purpose. In addition, for validated detection, measures to reduce the uncertainty of tests that are carried out need to be implemented through the whole diagnostic process, which must therefore also be robust.

The original version of this chapter was revised. Nataša Mehle’s affiliation was corrected. An erratum to this book can be found at DOI 10.1007/978-3-319-50648-7_5

An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-3-319-50648-7_5

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Acknowledgement

While the grafting experiments were partially supported by the grant P 24201-B16 of the Austrian Science Fund (FWF), the transmission ones were financed by the Euphresco project GRAFDEPI2 and by the Ministry of Agriculture, Forestry and Food of the Republic of Slovenia. We thank Mag. Gabrijel Seljak for helping in collecting and identification of O. ishidae, and Tina Naglič, and Špela Alič for the excellent technical help.

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Authors and Affiliations

  1. National Institute of Biology, Ljubljana, Slovenia

    Nataša Mehle, Maja Ravnikar, Magda Tušek Žnidarič & Marina Dermastia

  2. Bioresources Unit, Austrian Institute of Technology, Health & Environment Department, Tulln, Austria

    Amal Aryan & Günter Brader

  3. Department of Crop Sciences Division of Vegetables and Ornamentals, BOKU –University of Natural Resources and Life Sciences, Vienna, Austria

    Amal Aryan

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Mehle, N., Ravnikar, M., Žnidarič, M.T., Aryan, A., Brader, G., Dermastia, M. (2017). Detection of Phytoplasmas Associated to Grapevine Yellows Diseases in Research and Diagnostics. In: Grapevine Yellows Diseases and Their Phytoplasma Agents. SpringerBriefs in Agriculture. Springer, Cham. https://doi.org/10.1007/978-3-319-50648-7_4

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