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GARD: Genomic Allergen Rapid Detection

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Book cover Alternatives for Dermal Toxicity Testing

Abstract

Genomic allergen rapid detection (GARD) is an in vitro assay for the prediction of skin sensitizing capacity of chemicals. The GARD assay monitors changes in the expression of 196 genes, termed the GARD prediction signature. In short, MUTZ-3 cells, representing a well-characterized in vitro model of human dendritic cells (DCs), are exposed to the test substances for 24 h. After RNA extraction and analysis of expression levels, compounds are predicted as either sensitizers or non-sensitizers by a support vector machine model. Due to high information content, the test can deliver novel insights into pathway utilization and potency. Based on blinded test evaluations, GARD has been shown to have an accuracy of 89%.

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References

  1. OECD. ENV/JM/MONO(2012)10/PART1. The adverse outcome pathway for skin sensitisation initiated by covalent binding to proteins. 2012.

    Google Scholar 

  2. Johansson H, Albrekt AS, Lindstedt M, Borrebaeck CAK. A genomic biomarker signature can predict skin sensitizers using a cell-based in vitro alternative to animal tests. BMC Genomics. 2011;12:399.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Johansson H, Lindstedt M. Prediction of skin sensitizers using alternative methods to animal experimentation. Basic Clin Pharmacol. 2014;1(1):110–7.

    Article  CAS  Google Scholar 

  4. Lindstedt M, Borrebaeck CAK. Pattern rules: biomarker signatures for sensitization as an alternative to animal testing. Biomark Med. 2011;5(6):809–11.

    Article  PubMed  Google Scholar 

  5. Johansson H, Albrekt AS, Borrebaeck CAK, Lindstedt M. The GARD assay for assessment of chemical skin sensitizers. Toxicol In Vitro. 2013;27:1163–9.

    Article  CAS  PubMed  Google Scholar 

  6. Johansson H, Rydnert F, Kuehnl J, Schepky A, Borrebaeck CAK, Lindstedt M. GARD in-house validation – a proof of concept. Toxicol Sci. 2014;139(2):362–70.

    Article  CAS  PubMed  Google Scholar 

  7. Haneke KE, Tice RR, Carson BL, Margolin BH, Stokes WS. ICCVAM evaluation of the murine local lymph node assay. Data analyses completed by the national toxicology program interagency center for the evaluation of alternative toxicological methods. Regul Toxicol Pharmacol. 2001;34(3):274–86.

    Article  CAS  PubMed  Google Scholar 

  8. World Health Organization. Concise international chemical assessment document 48. 4-CHLORO-ANILINE, ISBN 9241530480. Geneva. 2003.

    Google Scholar 

  9. Basketter DA, Alépée N, Ashikaga T, Barroso J, Gilmour N, Goebel C, Hibatallah J, Hoffmann S, Kern P, Martinozzi-Teissier S, Maxwell G, Reisinger K, Sakaguchi H, Schepky A, Tailhardat M, Templier M. Categorization of chemicals according to their relative human skin sensitizing potency. Dermatitis. 2014;25(1):11–21.

    Article  CAS  PubMed  Google Scholar 

  10. EURL ECVAM. Summary sheet on the KeratinoSens assay prepared by EURL ECVAM. Update 14 Feb 2014. p. 4(34).

    Google Scholar 

  11. Forreryd A, Johansson H, Albrekt AS, Borrebaeck CAK, Lindstedt M. Prediction of chemical respiratory sensitizers using GARD, a novel in vitro assay based on a genomic biomarker signature. PLoS One. 2015;2015:11.

    Google Scholar 

  12. Forreryd A, Zeller KS, Johansson H, Lindstedt M. From genome-wide arrays to tailor-made biomarker readout – progress towards routine analysis of skin sensitizing chemicals with GARD. Toxicol In Vitro. 2016;37:178–88.

    Article  CAS  PubMed  Google Scholar 

  13. Forreryd A, Johansson H, Albrekt AS, Lindstedt M. Evaluation of high throughput gene expression platforms using GARD – an assay for prediction of skin sensitization based on a genomic biomarker signature. BMC Genomics. 2014;15(1):379.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Albrekt AS, Johansson H, Borrebaeck CAK, Lindstedt M. Differentially regulated signaling pathways in MUTZ-3 cells stimulated with skin sensitizers. BMC Pharmacol Toxicol. 2014;15:5.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Immunotechnology, Lund University, Medicon Village (Building 406), S-22183, Lund, Sweden

    Malin Lindstedt, Kathrin Stephanie Zeller & Carl Borrebaeck

  2. SenzaGen AB, Medicon Village (Building 401), S-22381, Lund, Sweden

    Henrik Johansson

Authors
  1. Malin Lindstedt
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  2. Kathrin Stephanie Zeller
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  3. Henrik Johansson
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  4. Carl Borrebaeck
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Corresponding author

Correspondence to Malin Lindstedt .

Editor information

Editors and Affiliations

  1. SeCAM, Magliaso, Switzerland

    Chantra Eskes

  2. Innovitox Consulting & Services, Houten, The Netherlands

    Erwin van Vliet

  3. Department of Dermatology, University of California School of Medicine, San Francisco, California, USA

    Howard I. Maibach

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© 2017 Springer International Publishing AG

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Lindstedt, M., Zeller, K.S., Johansson, H., Borrebaeck, C. (2017). GARD: Genomic Allergen Rapid Detection. In: Eskes, C., van Vliet, E., Maibach, H. (eds) Alternatives for Dermal Toxicity Testing. Springer, Cham. https://doi.org/10.1007/978-3-319-50353-0_27

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  • DOI: https://doi.org/10.1007/978-3-319-50353-0_27

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-50351-6

  • Online ISBN: 978-3-319-50353-0

  • eBook Packages: MedicineMedicine (R0)

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