Acne Research Models

  • Gerd Plewig
  • Bodo Melnik
  • WenChieh Chen


Genuine acne is a disease singular to man and practically does not occur in animals. There are a few exceptions to this rule. Except for minor forms in dogs and cats, acne does not occur spontaneously in any animal species. Acne is a uniquely human disorder. Certain species possess sebaceous-like follicles and are suitable for assessing the comedogenicity of chemicals, the comedolytic effects of drugs, and the androgen metabolism of sebocytes. We recognize the limitations; yet these models have proved to be valuable both in research and in pharmacological testing.


Canine Acne

  1. Bedord CJ, Young JM. A comparison of comedonal and skin surface lipids from hairless dogs showing clinical signs of acne. J Invest Dermatol. 1981;77:341–4.PubMedGoogle Scholar
  2. Bond R. Canine and feline acne. Vet Annu. 1993;33:230–5.Google Scholar
  3. Kimura T, Doi K. Spontaneous comedones on the skin of hairless descendants of Mexican hairless dogs. Exp Anim. 1996;45:377–84.PubMedGoogle Scholar
  4. Schwartzman RM, Kligman AM, Duclos DD. The Mexican hairless dog as a model for assessing the comedolytic and morphogenic activity of retinoids. Br J Dermatol. 1996;134:64–70.PubMedGoogle Scholar

Feline Acne

  1. Jazic E, Coyner KS, Loeffler DG, Lewis TP. An evaluation of the clinical, cytological, infectious and histopathological features of feline acne. Vet Dermatol. 2006;17:134–340.PubMedGoogle Scholar

Syrian Hamster

  1. Chen C, Li X, Singh SM, Labrie F. Activity of 17beta-(N-alkyl/arylformamido) and 17beta-[(N-alkyl/aryl) alkyl/arylamido]-4-methyl-4-aza-5alpha-androstan-3-ones as 5alpha-reductase inhibitors in the hamster flank organ and ear. J Invest Dermatol. 1998;111:273–8.PubMedGoogle Scholar
  2. Franz TJ, Lehman PA, Pochi P, et al. The hamster flank organ model: is it relevant to man? J Invest Dermatol. 1989;93:475–9.PubMedGoogle Scholar
  3. Hisaoka H, Ideta R, Seki T, Adachi K. Androgen regulation of a specific gene in hamster flank organs. Arch Dermatol Res. 1991;283:269–73.PubMedGoogle Scholar
  4. Hunt DW, Winters GC, Brownsey RW, et al. Inhibition of sebum production with the acetyl coenzyme A carboxylase inhibitor olumacostat glasaretil. J Invest Dermatol. 2017;137:1415–23.Google Scholar
  5. Li L, Tang L, Baranov E, et al. Selective induction of apoptosis in the hamster flank sebaceous gland organ by a topical liposome 5-alpha-reductase inhibitor: a treatment strategy for acne. J Dermatol. 2010;37:156–62.PubMedGoogle Scholar
  6. Lucky AW, McGuire J, Nydorf E, et al. Hair follicle response of the golden Syrian hamster flank organ to continuous testosterone stimulation using silastic capsules. J Invest Dermatol. 1986;86:83–6.PubMedGoogle Scholar
  7. Luderschmidt C, Plewig G. Effects of cyproterone acetate and carboxylic acid derivatives on the sebaceous glands of the Syrian hamster. Arch Dermatol Res. 1977;258:185–91.PubMedGoogle Scholar
  8. Luderschmidt C, Bidlingmaier F, Plewig G. Inhibition of sebaceous gland activity by spironolactone in Syrian hamster. J Invest Dermatol. 1982;78:253–5.PubMedGoogle Scholar
  9. Melnik BC. Olumacostat glasaretil, a promising topical sebum-suppressing agent that affects all major pathogenic factors of acne vulgaris. J Invest Dermatol. 2017a;137:1405–8.Google Scholar
  10. Plewig G, Luderschmidt C. Hamster ear model for sebaceous glands. J Invest Dermatol. 1977;68:171–6.PubMedGoogle Scholar
  11. Seki T, Ideta R, Shibuya M, Adachi K. Isolation and characterization of cDNA for an androgen-regulated mRNA in the flank organ of hamsters. J Invest Dermatol. 1991;96:926–31.PubMedGoogle Scholar
  12. Takayasu S, Adachi K. Hormonal control of metabolism in hamster costovertebral glands. J Invest Dermatol. 1970;55:13–9.PubMedGoogle Scholar
  13. Vermorken AJM, Goos CMAA, Wirtz P. Evaluation of the hamster flank organ test for the screening of anti-androgens. Br J Dermatol. 1982;106:99–101.PubMedGoogle Scholar

Rabbit Ear

  1. Adams EM, Irish DD, Spencer HC, Rowe VK. The response of rabbit skin to compounds reported to have caused acneiform dermatitis. Indust Med. 1941;10:1–4.Google Scholar
  2. American Academy of Dermatology Invitational Symposium on Comedogenicity. J Am Acad Dermatol. 1989;20:272–7.Google Scholar
  3. Fulton JE. Comedogenicity and irritancy of commonly used ingredients in skin care products. J Soc Cosmet Chem. 1989;40:321–33.Google Scholar
  4. Fulton JE, Pay SR, Fulton JE. Comedogenicity of current therapeutic products, cosmetics, and ingredients in the rabbit ear. J Am Acad Dermatol. 1984;10:96–105.PubMedGoogle Scholar
  5. Hambrick GW, Blank H. A microanatomical study of the response of the pilosebaceous apparatus of the rabbit’s ear canal. J Invest Dermatol. 1956;26:185–200.PubMedGoogle Scholar
  6. Kligman AM. Updating the rabbit ear comedogenic assay. In: Marks R, Plewig G, editors. Acne and related disorders. London: Dunitz; 1989. p. 97–106.Google Scholar
  7. Kligman AM, Kwong T. An improved rabbit ear model for assessing comedogenic substances. Br J Dermatol. 1979;100:699–702.PubMedGoogle Scholar
  8. Kwon TR, Choi EJ, Oh CT, et al. Targeting of sebaceous glands to treat acne by micro-insulated needles with radio frequency in a rabbit ear model. Lasers Surg Med. 2017;49:395–401.PubMedGoogle Scholar
  9. Lanzet M. Comedogenic effects of cosmetic raw materials. Cosmet Toilet. 1986;101:63–72.Google Scholar
  10. Mezick JA, Thorne EG, Bhatia MC, et al. The rabbit ear microcomedo prevention assay. A new model to evaluate anti-acne agents. In: Maibach HI, Lowe NJ, editors. Models in dermatology, vol. 3. Basel: Karger; 1987. p. 68–73.Google Scholar
  11. Mills OH Jr, Kligman AM. A human model for assessing comedogenic substances. Arch Dermatol. 1982;118:903–5.PubMedGoogle Scholar
  12. Mirshahpanah P, Maibach HI. Models in acnegenesis. Cutan Ocul Toxicol. 2007;26:195–202.PubMedGoogle Scholar
  13. Morris WE, Kwan SC. Use of the rabbit ear model in evaluating the comedogenic potential of cosmetic ingredients. J Soc Cosmet Chem. 1983;34:215–25.Google Scholar
  14. Nguyen SH, Dang TP, Maibach HI. Comedogenicity in rabbit: some cosmetic ingredients/vehicles. Cutan Ocul Toxicol. 2007;26:287–92.Google Scholar
  15. Wang Q, Jiang C, Liu W, et al. A new optical intra-tissue fiber irradiation ALA-PDT in the treatment of acne vulgaris in rabbit model: improved safety and tolerability. An Bras Dermatol. 2017;92:350–5.PubMedPubMedCentralGoogle Scholar

Rhino Mouse

  1. Ashton RE, Connor MJ, Lowe NJ. Histologic changes in the skin of the rhino mouse induced by retinoids. J Invest Dermatol. 1984;82:632–5.PubMedGoogle Scholar
  2. Bernerd F, Demarchez M, Ortonne JP, Czernielewski J. Sequence of morphological events during topical application of retinoic acid on the rhino mouse skin. Br J Dermatol. 1991a;125:419–25.PubMedGoogle Scholar
  3. Bernerd F, Ortonne JP, Bouclier M, et al. The rhino mouse model: the effects of topically applied all-trans retinoic acid and CD271 on the fine structure of the epidermis and utricle wall of pseudocomedones. Arch Dermatol Res. 1991b;283:100–7.PubMedGoogle Scholar
  4. Bouclier M, Chatelus A, Ferracin J, et al. Quantification of epidermal histological changes induced by topical retinoids and CD271 in the rhino mouse model using a standardized image analysis technique. Skin Pharmacol. 1991;4:65–73.PubMedGoogle Scholar
  5. Gaskoyne JS. On a peculiar variety of Mus musculus. Proc Zool Soc Lond. 1856;24:38–40.Google Scholar
  6. Hayashi N, Watanabe H, Yasukawa H, et al. Comedolytic effect of topically applied active vitamin D3 analogue on pseudocomedones in the rhino mouse. Br J Dermatol. 2006;155:895–901.PubMedGoogle Scholar
  7. Kligman LH, Kligman AM. The effect on rhino mouse skin of agents which influence keratinization and exfoliation. J Invest Dermatol. 1979;73:354–8.PubMedGoogle Scholar
  8. Lowe NJ, Weingarten D. The effects of hyperproliferative agents on the rhino mouse: variable effects on keratin utricles. In: Marks R, Plewig G, editors. Acne and related disorders. London: Dunitz; 1989. p. 165–7.Google Scholar
  9. Mann SJ. Hair loss and cyst formation in hairless and rhino mutant mice. Anat Rec. 1971;170:485–99.PubMedGoogle Scholar
  10. Mezick JA, Bhatia MC, Capetola RJ. Topical and systemic effects of retinoids on horn-filled utriculus size in the rhino mouse: a model to quantify “antikeratinizing” effects of retinoids. J Invest Dermatol. 1984;83:110–3.PubMedGoogle Scholar
  11. Nakano K, Kiyokane K, Benvenuto-Andrade C, et al. Real-time reflectance confocal microscopy, a noninvasive tool for in vivo quantitative evaluation of comedolysis in the rhino mouse model. Skin Pharmacol Physiol. 2007;20:29–36.PubMedGoogle Scholar
  12. Odorisio T, De Luca N, Vesci L, et al. The atypical retinoid E-3-(3′-Adamantan-1-yl-4′-methoxybiphenyl-4-yl)-2-propenoic acid (ST1898) displays comedolytic activity in the rhino mouse model. Eur J Dermatol. 2012;22:505–11.PubMedGoogle Scholar
  13. Sakuta T, Kanayama T. Comedolytic effect of a novel RARgamma-specific retinoid, ER36009: comparison with retinoic acid in the rhino mouse model. Eur J Dermatol. 2005;15:459–64.PubMedGoogle Scholar
  14. Seiberg M, Siock P, Wisniewski S, et al. The effects of trypsin on apoptosis, utriculi size, and skin elasticity in the rhino mouse. J Invest Dermatol. 1997;109:370–6.PubMedGoogle Scholar

HR-1 Mice

  1. Jang YH, Lee KC, Lee SJ, et al. HR-1 Mice: a new inflammatory acne mouse model. Ann Dermatol. 2015;27:257–64.PubMedPubMedCentralGoogle Scholar
  2. Lee WJ, Lee KC, Kim MJ, et al. Efficacy of red or infrared light-emitting diodes in a mouse model of Propionibacterium acnes-induced inflammation. Ann Dermatol. 2016;28:186–91.PubMedPubMedCentralGoogle Scholar

The Human Sebaceous Gland Organ Culture

  1. Downie MM, Kealey T. Human sebaceous glands engage in aerobic glycolysis and glutaminolysis. Br J Dermatol. 2004;151:320–7.PubMedPubMedCentralGoogle Scholar
  2. Downie MM, Sanders DA, Maier LM, et al. Peroxisome proliferator-activated receptor and farnesoid X receptor ligands differentially regulate sebaceous differentiation in human sebaceous gland organ cultures in vitro. Br J Dermatol. 2004;151:766–75.PubMedGoogle Scholar
  3. Guy R, Ridden C, Barth J, Kealey T. Isolation and maintenance of the human pilosebaceous duct: 13-cis retinoic acid acts directly on the duct in vitro. Br J Dermatol. 1993;128:242–8.PubMedGoogle Scholar
  4. Guy R, Kealey T. The organ-maintained human sebaceous gland. Dermatology. 1998;196:16–20.PubMedGoogle Scholar
  5. Kellum RE. Isolation of human sebaceous glands. Arch Dermatol. 1966;93:610–2.PubMedGoogle Scholar
  6. Philpott MP. Culture of the human pilosebaceous unit, hair follicle and sebaceous gland. Exp Dermatol. 2018;27:571–7.PubMedGoogle Scholar
  7. Ridden J, Ferguson D, Kealey T. Organ maintenance of human sebaceous glands: in vitro effects of 13-cis retinoic acid and testosterone. J Cell Sci. 1990;95(Pt 1):125–36.PubMedGoogle Scholar
  8. Sanders DA, Philpott MP, Nicolle FV, Kealey T. The isolation and maintenance of the human pilosebaceous unit. Br J Dermatol. 1994;131:166–76.PubMedGoogle Scholar
  9. Thiboutot DM, Knaggs H, Gilliland K, Hagari S. Activity of type 1 5 alpha-reductase is greater in the follicular infrainfundibulum compared with the epidermis. Br J Dermatol. 1997;136:166–71.PubMedGoogle Scholar

Primary Human Sebocyte Cultures

  1. Abdel-Naser MB. Selective cultivation of normal human sebocytes in vitro; a simple modified technique for a better cell yield. Exp Dermatol. 2004;13:562–6.PubMedGoogle Scholar
  2. Akamatsu H, Zouboulis CC, Orfanos CE. Control of human sebocyte proliferation in vitro by testosterone and 5-alpha-dihydrotestosterone is dependent on the localization of the sebaceous glands. J Invest Dermatol. 1992;99:509–11.PubMedGoogle Scholar
  3. Doran TI, Baff R, Jacobs P, Pacia E. Characterization of human sebaceous cells in vitro. J Invest Dermatol. 1991;96:341–8.PubMedGoogle Scholar
  4. Fujie T, Shikiji T, Uchida N, et al. Culture of cells derived from the human sebaceous gland under serum-free conditions without a biological feeder layer or specific matrices. Arch Dermatol Res. 1996;288:703–8.PubMedGoogle Scholar
  5. Karasek M, Charlton M. Isolation and growth of rabbit and human sebaceous gland cells in cell culture. J Invest Dermatol. 1977;68:234A.Google Scholar
  6. Karasek MA, Charlton ME. In vitro growth and serial cultivation of normal human sebaceous gland cells. Clin Res. 1982;30:263A.Google Scholar
  7. Kim H, Moon SY, Sohn MY, Lee WJ. Insulin-like growth factor-1 increases the expression of inflammatory biomarkers and sebum production in cultured sebocytes. Ann Dermatol. 2017;29:20–5.PubMedPubMedCentralGoogle Scholar
  8. Laptenko O, Prives C. p53: master of life, death, and the epigenome. Genes Dev. 2017;31:955–6.PubMedPubMedCentralGoogle Scholar
  9. Lee CM. Cell culture systems for the study of human skin and skin glands. In: Jones CJ, editor. Epithelia: advances in cell physiology and cell culture. Dordrecht: Kluwer; 1990. p. 70–350.Google Scholar
  10. Rosenfield RL. Relationship of sebaceous cell stage to growth in culture. J Invest Dermatol. 1989;92:751–4.PubMedGoogle Scholar
  11. Rosenfield R. Preputial cell culture as a model system to study sebocyte development. In: Van Neste D, Randall V, editors. Hair research for the next millenium. Amsterdam: Elsevier ScienceBV; 1996. p. 375–9.Google Scholar
  12. Schneider MR, Zouboulis CC. Primary sebocytes and sebaceous gland cell lines for studying sebaceous lipogenesis and sebaceous gland diseases. Exp Dermatol. 2018;27:484–8.PubMedGoogle Scholar
  13. Wheatley VR, Potter JE, Lew G. Sebaceous gland differentiation: II. The isolation, separation and characterization of cells from the mouse preputial gland. J Invest Dermatol. 1979;73:291–6.PubMedGoogle Scholar
  14. Xia L, Zouboulis CC, Orfanos CE. Isolation of human sebaceous glands and cultivation of cells presenting evidence for sebocytic differentiation in vitro. J Invest Dermatol. 1989a;92:544A.Google Scholar
  15. Xia L, Zouboulis CC, Detmar M, et al. Isolation of human sebaceous glands and cultivation of sebaceous gland-derived cells as an in vitro model. J Invest Dermatol. 1989b;93:314–21.Google Scholar
  16. Xia L, Zouboulis CC, Ju Q. Culture of human sebocytes in vitro. Dermatoendocrinol. 2009;1:92–5.PubMedPubMedCentralGoogle Scholar
  17. Zouboulis CC, Korge B, Akamatsu H, et al. Effects of 13-cis-retinoic acid, all-trans-retinoic acid, and acitretin on the proliferation, lipid synthesis and keratin expression of cultured human sebocytes in vitro. J Invest Dermatol. 1991a;96:792–7.PubMedGoogle Scholar
  18. Zouboulis CC, Xia LQ, Detmar M, et al. Culture of human sebocytes and markers of sebocytic differentiation in vitro. Skin Pharmacol. 1991b;4:74–83.PubMedGoogle Scholar
  19. Zouboulis CC. Sebaceous cells in monolayer culture. In Vitro Cell Dev Biol. 1992;28A:699.PubMedGoogle Scholar
  20. Zouboulis CC, Xia L, Akamatsu H, et al. The human sebocyte culture model provides new insights into development and management of seborrhoea and acne. Dermatology. 1998;196:21–31.PubMedGoogle Scholar
  21. Zouboulis CC, Korge B, Giannakopoulos G, et al. Cultured human sebocytes possess a characteristic pattern of sebocytic differentiation in vitro. J Invest Dermatol. 1990;95:496.Google Scholar

Immortalized Human Sebocyte Cultures

  1. Alimirah F, Panchanathan R, Chen J, et al. Expression of androgen receptor is negatively regulated by p53. Neoplasia. 2007;9:1152–9.PubMedPubMedCentralGoogle Scholar
  2. Bocchetta M, Eliasz S, De Marco MA, et al. The SV40 large T antigen-p53 complexes bind and activate the insulin-like growth factor-I promoter stimulating cell growth. Cancer Res. 2008;68:1022–9.PubMedGoogle Scholar
  3. Budanov AV. Stress-responsive sestrins link p53 with redox regulation and mammalian target of rapamycin signaling. Antioxid Redox Signal. 2011;15:1679–90.PubMedPubMedCentralGoogle Scholar
  4. Deplewski D, Rosenfield RL. Growth hormone and insulin-like growth factors have different effects on sebaceous cell growth and differentiation. Endocrinology. 1999;140:4089–94.PubMedPubMedCentralGoogle Scholar
  5. Dobbelstein M, Roth J. The large T antigen of simian virus 40 binds and inactivates p53 but not p73. J Gen Virol. 1998;79:3079–83.PubMedGoogle Scholar
  6. Feng Z. p53 regulation of the IGF-1/AKT/mTOR pathways and the endosomal compartment. Cold Spring Harb Perspect Biol. 2010;2:a001057.PubMedPubMedCentralGoogle Scholar
  7. Feng Z, Levine AJ. The regulation of energy metabolism and the IGF-1/mTOR pathways by the p53 protein. Trends Cell Biol. 2010;20:427–34.PubMedPubMedCentralGoogle Scholar
  8. Fischer M. Census and evaluation of p53 target genes. Oncogene. 2017;36:3943–56.PubMedPubMedCentralGoogle Scholar
  9. Fischer M, Uxa S, Stanko C, et al. Human papilloma virus E7 oncoprotein abrogates the p53-p21-DREAM pathway. Sci Rep. 2017;7:2603.PubMedPubMedCentralGoogle Scholar
  10. Flöter J, Kaymak I, Schulze A. Regulation of metabolic activity by p53. Meta. 2017;7:21.Google Scholar
  11. Gnanapradeepan K, Basu S, Barnoud T, et al. The p53 tumor suppressor in the control of metabolism and ferroptosis. Front Endocrinol (Lausanne). 2018;9:124.Google Scholar
  12. Hay N. p53 strikes mTORC1 by employing sestrins. Cell Metab. 2008;8:184–5.PubMedPubMedCentralGoogle Scholar
  13. Humpton TJ, Vousden KH. Regulation of cellular metabolism and hypoxia by p53. Cold Spring Harb Perspect Med. 2016;6:a026146.PubMedPubMedCentralGoogle Scholar
  14. Jiang D, Srinivasan A, Lozano G, Robbins PD. SV40 T antigen abrogates p53-mediated transcriptional activity. Oncogene. 1993;8:2805–12.PubMedGoogle Scholar
  15. Jiang L, Hickman JH, Wang SJ, Gu W. Dynamic roles of p53-mediated metabolic activities in ROS-induced stress responses. Cell Cycle. 2015;14:2881–5.PubMedPubMedCentralGoogle Scholar
  16. Jha KK, Banga S, Palejwala V, Ozer HL. SV40-mediated immortalization. Exp Cell Res. 1998;245:1–7.PubMedGoogle Scholar
  17. Lo Celso C, Berta MA, Braun KM, et al. Characterization of bipotential epidermal progenitors derived from human sebaceous gland: contrasting roles of c-Myc and beta-catenin. Stem Cells. 2008;26:1241–52.PubMedGoogle Scholar
  18. Maddocks OD, Vousden KH. Metabolic regulation by p53. J Mol Med (Berl). 2011;89:237–45.Google Scholar
  19. McCormick F, Clark R, Harlow E, Tjian R. SV40 T antigen binds specifically to a cellular 53 K protein in vitro. Nature. 1981;292:63–5.PubMedGoogle Scholar
  20. Melnik BC. Isotretinoin and FoxO1: a scientific hypothesis. Dermatoendocrinol. 2011;3:141–65.PubMedPubMedCentralGoogle Scholar
  21. Melnik BC. Apoptosis may explain the pharmacological mode of action and adverse effects of isotretinoin, including teratogenicity. Acta Derm Venereol. 2017b;97:173–81.PubMedGoogle Scholar
  22. Melnik BC. The TRAIL to acne pathogenesis: let’s focus on death pathways. Exp Dermatol. 2017c;26:270–2.PubMedGoogle Scholar
  23. Melnik BC. p53: key conductor of all anti-acne therapies. J Transl Med. 2017d;15:195.PubMedPubMedCentralGoogle Scholar
  24. Melnik BC, John SM, Agamia NF, et al. Isotretinoin’s paradoxical effects in immortalized sebocytes. Br J Dermatol. 2019;180:957–8.PubMedGoogle Scholar
  25. Mirdamadi Y, Thielitz A, Wiede A, et al. Effects of isotretinoin on the phosphoinositide-3-kinase/Akt/FoxO1 pathway and molecular functions of SZ95 sebocytes in vitro. J Clin Exp Dermatol Res. 2017;8:3.Google Scholar
  26. Nelson AM, Gilliland KL, Cong Z, Thiboutot DM. 13-cis Retinoic acid induces apoptosis and cell cycle arrest in human SEB-1 sebocytes. J Invest Dermatol. 2006;126:2178–89.PubMedGoogle Scholar
  27. Nelson AM, Cong Z, Gilliland KL, Thiboutot DM. TRAIL contributes to the apoptotic effect of 13-cis retinoic acid in human sebaceous gland cells. Br J Dermatol. 2011;165:526–33.PubMedPubMedCentralGoogle Scholar
  28. Nikolakis G, Seltmann H, Hossini AM, et al. Ex vivo human skin and SZ95 sebocytes exhibit a homoeostatic interaction in a novel coculture contact model. Exp Dermatol. 2015;24:497–502.PubMedGoogle Scholar
  29. Shi G, Liao PY, Cai XL, et al. FoxO1 enhances differentiation and apoptosis in human primary keratinocytes. Exp Dermatol. 2018;27:1254–60.PubMedGoogle Scholar
  30. Thiboutot D, Jabara S, McAllister JM, et al. Human skin is a steroidogenic tissue: steroidogenic enzymes and cofactors are expressed in epidermis, normal sebocytes, and an immortalized sebocyte cell line (SEB-1). J Invest Dermatol. 2003;120:905–14.PubMedGoogle Scholar
  31. Werner H, Karnieli E, Rauscher FJ, LeRoith D. Wild-type and mutant p53 differentially regulate transcription of the insulin-like growth factor I receptor gene. Proc Natl Acad Sci U S A. 1996;93:8318–23.PubMedPubMedCentralGoogle Scholar
  32. Wróbel A, Seltmann H, Fimmel S, et al. Differentiation and apoptosis in human immortalized sebocytes. J Invest Dermatol. 2003;120:175–81.PubMedGoogle Scholar
  33. Zhang XD, Qin ZH, Wang J. The role of p53 in cell metabolism. Acta Pharmacol Sin. 2010;31:1208–12.PubMedPubMedCentralGoogle Scholar
  34. Zouboulis CC, Seltmann H, Neitzel H, Orfanos CE. Establishment and characterization of an immortalized human sebaceous gland cell line (SZ95). J Invest Dermatol. 1999;113:1011–20.PubMedPubMedCentralGoogle Scholar
  35. Zouboulis CC, Schagen S, Alestas T. The sebocyte culture: a model to study the pathophysiology of the sebaceous gland in sebostasis, seborrhoea and acne. Arch Dermatol Res. 2008;300:397–413.PubMedGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Gerd Plewig
    • 1
  • Bodo Melnik
    • 2
  • WenChieh Chen
    • 3
  1. 1.Department of Dermatology and AllergyLudwig-Maximilian-University MunichMunichGermany
  2. 2.Department of Dermatology, Environmental Medicine and Health TheoryUniversity of OsnabrückOsnabrückGermany
  3. 3.Department of Dermatology and AllergyTechnical University of MunichMunichGermany

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