Abstract
Conservation and maintenance strategies for microorganisms are essential to develop the biotechnology and microbiology of twenty-first century. It is necessary to develop methodologies to keep stable and safely each strain isolated or developed in a laboratory and to be able to keep the microbes exactly in the same conditions that they were found or selected. In the case of fungi, especially in non-sporulated, conservation and maintenance are more complicated than other microbes, due to the difficulty of lyophilizing it. Other times, it is necessary to consider that there are laboratories where it is not possible to lyophilize the samples or keep them in safety freezer due to the lack of facilities. That is why here we presented a simply methodology to find the best conservation method for a new fungus, according to the conditions of laboratories with different budgets and facilities. Along the chapter, a methodology to be followed by a researcher who has to find the more suitable method to conserve new fungus, adapting the method to the fungi characteristic and laboratory restrictions, is presented. Piriformospora indica, a recently discovered and promising fungus from the point of view of plant–microbe interaction biotechnology, is taken as a successful conservation case.
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Caleza, V. et al. (2017). Conservation Strategies of New Fungi Samples in Culture Collections: Piriformospora indica Case. In: Varma, A., Sharma, A. (eds) Modern Tools and Techniques to Understand Microbes. Springer, Cham. https://doi.org/10.1007/978-3-319-49197-4_27
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DOI: https://doi.org/10.1007/978-3-319-49197-4_27
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