Abstract
Microbial proteins are diverse and fascinating matter to study. Majority of time it happens that a protein cannot be studied at fine details as its properties are masked by others in vicinity. For the same, purification to homogeneity is required, and there are enormous number of techniques for protein purification currently followed in various laboratories. We will discuss one of them here. This chapter focuses on purification of protein sample from fungal sources to homogeneity by continuous elution electrophoresis (CEE) using Miniprep Cell. This is an extremely reproducible technique for getting single protein fractions during purification steps and works beyond doubt for separating two different proteins which resemble each other in all respect except a difference of few Daltons. In the present review, we will highlight the importance of continuous elution electrophoresis in separating biologically active proteins.
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Acknowledgments
Dr. Smriti Shrivastava is thankful to the Science and Engineering Research Board for providing her fellowship and research grant (SR/FT/LS-171/2012).
Ajit Varma is thankful to Department of Science and Technology and Department of Biotechnology for partial financial funding and to DST-FIST for providing confocal microscope facility.
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Shrivastava, S., Shukla, P., Mukhopadhyay, K., Varma, A. (2017). Continuous Elution Electrophoresis: A Unique Tool for Microbial Protein Analysis. In: Varma, A., Sharma, A. (eds) Modern Tools and Techniques to Understand Microbes. Springer, Cham. https://doi.org/10.1007/978-3-319-49197-4_14
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DOI: https://doi.org/10.1007/978-3-319-49197-4_14
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