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Introduction to Modern Tools and Techniques to Understand Microbes

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Modern Tools and Techniques to Understand Microbes

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Abstract

Microorganisms are omnipresent in the nature and having both positive and negative effects on various aspects of human life and society. To study their impact on environment and other associated fields, it is crucial to be able to cultivate the microbes under laboratory conditions and to detect and identify them quickly and accurately. However, difficulties in cultivating most of the microbes by standard traditional methods such as plate count, filteration, etc., limit our ability to study these tiny organisms. To enhance the microbial studies effectively particularly for microbes which are viable but non-culturable (VBNC), several modern approaches such as molecular methods are proving to be appreciated. The most appreciable modern molecular methods which are used to detect and identify organisms include rRNA or rDNA fingerprinting, probing, and sequencing.

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Acknowledgment

Ajit Varma is thankful to Department of Science and Technology and Department of Biotechnology for partial financial funding and to DST-FIST for providing confocal microscope facility.

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Authors and Affiliations

  1. Amity Institute of Microbial Technology, Amity University Uttar Pradesh, Noida, UP, 201303, India

    Pankaj Goyal, Diksha Bhola & Ajit Varma

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  1. Pankaj Goyal
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  2. Diksha Bhola
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  3. Ajit Varma
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Correspondence to Ajit Varma .

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  1. Amity Institute of Microbial Technology, Amity University Uttar Pradesh, Noida, Uttar Pradesh, India

    Ajit Varma

  2. ICAR-National Bureau of Agriculturally Important Microorganisms (NBAIM), Kusmaur, Mau Nath Bhanjan, Uttar Pradesh, India

    Arun Kumar Sharma

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Goyal, P., Bhola, D., Varma, A. (2017). Introduction to Modern Tools and Techniques to Understand Microbes. In: Varma, A., Sharma, A. (eds) Modern Tools and Techniques to Understand Microbes. Springer, Cham. https://doi.org/10.1007/978-3-319-49197-4_1

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