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Mathematical Modeling of the DNA Double-Strand Break Repair in Mammalian and Human Cells

  • Oleg V. BelovEmail author
  • Marina S. Panina
  • Munkhbaatar Batmunkh
  • Nasser Sweilam
Chapter

Abstract

A numerical model is offered to simulate the major pathways of DNA double-strand break. It provides a possible mechanistic explanation of the basic regularities of DSB processing by means of the non-homologous end-joining (NHEJ), homologous recombination (HR), single-strand annealing (SSA) and two alternative end-joining pathways. The model reproduces the time-courses of radiation-induced fluorescent foci specific to particular repair processes. It was tested for a wide spectrum of radiations with different linear energy transfer values ranged from 0.2 to 236 keV/μm. Using the proposed approach, we have reproduced several experimental data sets on γ-H2AX foci remaining in different types of cells including those defective in NHEJ, HR, or SSA functions. The results produced meet the hypothesis that the alternative end-joining pathways represented by micro-SSA and Alt-NHEJ can eliminate some amount of DSBs when classical NHEJ fails.

Keywords

DNA double-strand break repair Ionizing radiation Mathematical modeling 

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Copyright information

© Springer International Publishing AG 2016

Authors and Affiliations

  • Oleg V. Belov
    • 1
    Email author
  • Marina S. Panina
    • 1
  • Munkhbaatar Batmunkh
    • 1
    • 2
  • Nasser Sweilam
    • 3
  1. 1.Laboratory of Radiation Biology, Joint Institute for Nuclear ResearchDubnaRussia
  2. 2.National University of MongoliaUlaanbaatarMongolia
  3. 3.Mathematics Department, Faculty of ScienceCairo UniversityGizaEgypt

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