Abstract
The study has been attempted as a combined process to treat groundwater contamination with nitrate through lab scale bioreactor using dissimilatory denitrifying bacterium Bacillus weihenstephanensis (DS45) and chemical treatments by alum and lime. Nitrate removal was attained up to 80.04% at 156 h by bioreactor process. The residual nitrate, nitrite, ammonium and bacterial growth were effectively removed by addition of lime at 150 mg/L as active coagulants. The treated sample was applied to various disinfectants such as boiling, membrane filtration, UV radiation and chlorination in order to remove bacterial biomass. The bacterial cells of 17 × 104 CFU/ml was completely removed by various disinfectant methods.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
APHA (2005) Standard methods for the examination of water and wastewater, 21st American Public Health Association, Baltimore, Mary, USA
Ayyasamy PM, Shanthi K, Lakshmanaperumalsamy P, Lee S, Choi N, Kim D (2007) Two-stage removal of nitrate from groundwater using biological and chemical treatments. J Biosci Bioeng 104(2):129–134
Barana AC, Lopes DD, Martins TH, Pozzi E, Damianovic MHRZ, Del Nery V, Foresti E (2013) Nitrogen and organic matter removal in an intermittently aerated fixed-bed reactor for post-treatment of anaerobic effluent from a slaughterhouse wastewater treatment plant. J Environ Chem Eng 1:453–459
Buttiglieri G, Malp F, Daveri E, Melchiori M, Nieman H, Ligthart J (2005) Denitrification of drinking water sources by advanced biological treatment using a membrane bioreactor. Desalination 178:211–218
Chen Q, Ni J (2011) Heterotrophic nitrification–aerobic denitrification by novel isolated bacteria. J Ind Microbiol Biotechnol 38(9):1305–1310
Chen F, Xia Q, Ju LK (2003) Aerobic denitrification of Pseudomonas aeruginosa monitored by online NAD(P)H fluorescence. App Environ Microbiol 69:6715–6722
Chen P, Li J, Li QX, Wang Y, Li S, Ren T, Wang L (2012) Simultaneous heterotrophic nitrification and aerobic denitrification by Bacterium Rhodococcus sp. CPZ24. Bioresour Technol 116:266–270
Chitra AV, Lakshmanaperumalsamy P (2006) Biodegradation of nitrate in waste streams from explosives manufacturing plants. Res J Microbiol 1:142–151
Choi YC, Li X, Raskin L, Morgenroth E (2007) Effect of backwashing on perchlorate removal in fixed bed Biofilm reactors. Water Res 41(9):1949–1959
Craun GF (1986) Water borne diseases in the United States. CRC Press, Boca Raton, FL
Ergas SJ, Rheinheimer DE (2004) Drinking water denitrification using a membrane bioreactor. Water Res 38:3225–3232
Espinosa LM, Stephenson T (1999) A review of biological aerated filters (BAFs) for wastewater treatment. Environ Eng Sci 16(3):201–216
Fernandez-Nava Y, Maranon E, Soons J, Castrillon L (2010) Denitrification of high nitrate concentration wastewater using alternative carbon sources. J Hazard Mater 173(1–3):682–688
Fu Z, Yang F, An Y, Xue Y (2009) Simultaneous nitrification and denitrifications coupled with phosphorus removal in an modified anoxic/oxic-membrane bioreactor (A/O-MBR). Biochem Eng J 43(2):191–196
Guo L, Chen Q, Fang F, Hu Z, Wu J, Miao A, Xiao L, Chen X, Yang L (2013) Application potential of a newly isolated indigenous aerobic denitrifier for nitrate and ammonium removal of eutrophic lake water. Bioresour Technol 142:45–51
Harm W (1980) Biological effects of ultraviolet radiation. In: International union of pure and applied biophysics, Biophysics series. Cambridge University press, Cambridge
Hem JD (1992) Study and interpretation of the chemical characteristics of nature water, USGS water supply paper 2254, 3rd edn. U.S. Government Printing Office, Washington, D.C.
Jiang JQ, Graham NJD (1998) Pre-polymerized inorganic coagulants and phosphorous removal by coagulation—a review. Water SA 24:237–244
Kesseru P, Kiss I, Bihari Z, Polyak B (2002) Investigation of the denitrification activity of immobilized Pseudomonas butanovora cells in the presence of different organic substrates. Water Res 36:1565–1571
Kross BC, Ayebo AD, Fuortes LJ (1992) Methaemoglobinaemia: nitrate toxicity in rural America. Am Fam Physician 46:183–188
Lee JW, Lee KH, Park KY (2010) Hydrogenotrophic denitrification in a packed bed reactor: effects of hydrogen-to-water flow rate ratio. Bioresour Technol 101:3940–3946
Liu M, Ming J, Ankumah RO (2005) Nitrate contamination in private wells in rural Alabama, United States. Sci Total Environ 346:112–120
Lunkad SK (1994) Rising nitrate levels in ground water and increasing N fertilizer consumption. Bhul Jal 9:4–10
McAdam EJ, Judd SJ (2006) A review of membrane bioreactor potential for nitrate removal from drinking water. Desalination 196:135–148
McAdam EJ, Judd SJ (2007) Denitrification from drinking water using a membrane bioreactor: chemical and biochemical feasibility. Water Res 41(18):4242–4250
Metcalf and Eddy (2003) Wastewater engineering treatment and reuse. McGraw-Hill, New York, NY
Nameni M, Mghadam MR, Arami M (2008) Adsorption of hexavalent chromium from aqueous solutions by wheat bran. Int J Environ Sci Technol 5(2):161–168
Ogata F, Imai D, Kawasaki D (2015) Adsorption of nitrate and nitrite ions onto carbonaceous material produced from soybean in a binary solution system. J Environ Chem Eng 3:155–161
Okeola FO, Odebunmi EO (2010) Comparison of Freundlich and Langmuir isotherms for adsorption of methylene blue by agrowaste derived activated carbon. Adv Environ Biol 4(3):329–335
Pauwels H, Talbo H (2004) Nitrates concentration in wetlands: assessing the contribution of different water bodies from anion concentrations. Water Res 38:1019–1025
Potter P, Ramankutty N et al (2010) Global fertilizer application and manure production. The Trustees of Columbia University in the City of New York, New York
Ravnjak M, Vrtovsek J, Pintar A (2013) Denitrification of drinking water in a two-stage membrane bioreactor by using immobilized biomass. Bioresour Technol 128:804–808
Rice EW, Hoff JC (1981) Inactivation of Giardia lamblia cysts by ultra violet radiation. Appl Environ Microbiol 42:546–547
Sadiq R, Rodriguez MJ (2004) Fuzzy synthetic evaluation of disinfection by-products—a risk-based indexing system. J Environ Manage 73(1):1–13
Savithamani K (2002), Seasonal distribution of Aeromonas spp. in river Amaravathy—Tamil Nadu. Ph.D. thesis, Bharathiar University, Coimbatore, Tamil Nadu
Seenivasagan R, Rajakumar S, Kasimani R, Ayyasamy PM (2014) Screening of assimilatory and dissmilatory Denitrifying microbes isolated from nitrate Contaminated water and soil. Prep Biochem Biotechnol 44(6):586–597
Shen J, He R, Han W, Sun X, Li J, Wang L (2009) Biological denitrification of high-nitrate wastewater in a modified anoxic/oxic-membrane bioreactor (A/OMBR). J Hazard Mater 172(2–3):595–600
Shen Z, Zhou Y, Wang J (2013) Comparison of denitrification performance and microbial diversity using starch/polylactic acid blends and ethanol as electron donor for nitrate removal. Bioresour Technol 131:33–39
Song H, Yao Z, Wang M, Wang J, Zhu Z, Li A (2013) Effect of dissolved organic matter on nitrate-nitrogen removal by anion exchange resin and kinetics studies. J Environ Sci 25(1):105–113
Trussell RR (1993) Treatment for the control of disinfectant byproducts and disinfectant residuals. In: Craun GF (ed) Safety of water disinfection: balancing chemical and microbial risks. International Life Science Institute (ILSI), Washington, D.C., USA
Wolfe RL (1990) Ultraviolet disinfection of potable water. Environ Sci Technol 24:769–773
Zhang J, Wu P, Hao B, Yu Z (2011) Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001. Bioresour Technol 102(21):9866–9869
Zhang QL, Liu Y, Ai GM, Miao LL, Zheng HY, Liu ZP (2012) The characteristics of a novel heterotrophic nitrification–aerobic denitrification bacterium, Bacillus methylotrophicus strain L7. Bioresour Technol 108:35–44
Acknowledgements
The authors are thankful to University Grants commission (UGC), New Delhi, India, for providing financial support for this research with vide reference to UGC letter F. No: 37-302/2009 (SR) dated 31.3.2010.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer International Publishing AG
About this chapter
Cite this chapter
Seenivasagan, R., Ayyasamy, P.M., Kasimani, R., Karthika, A., Rajakumar, S., Babalola, O.O. (2017). Nitrate Removal from Ground Water Through Lab Scale Bioreactor Using Dissimilatory Nitrate Reducer Bacillus weihenstephanensis (DS45). In: Prashanthi, M., Sundaram, R., Jeyaseelan, A., Kaliannan, T. (eds) Bioremediation and Sustainable Technologies for Cleaner Environment. Environmental Science and Engineering(). Springer, Cham. https://doi.org/10.1007/978-3-319-48439-6_8
Download citation
DOI: https://doi.org/10.1007/978-3-319-48439-6_8
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-48438-9
Online ISBN: 978-3-319-48439-6
eBook Packages: Earth and Environmental ScienceEarth and Environmental Science (R0)