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Spatial Metabolite Profiling by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging

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Metabolomics: From Fundamentals to Clinical Applications

Part of the book series: Advances in Experimental Medicine and Biology ((PMISB,volume 965))

Abstract

Mass spectrometry imaging (MSI) is rapidly maturing as an advanced method for spatial metabolite profiling. Herein, we provide an introduction to MSI including types of instrumentation, detailed sample preparation, data collection, overview of data analysis steps, software, common standards, and new developments. Further, we provide an overview of MSI in the clinical space over the past 3 years where MSI has been deployed in diverse research areas including cancer, neurobiology, lipidomics, and metabolite profiling and mapping to name only a few. We provide several examples demonstrating the applicability of MSI to spatially profile metabolites in unique systems requiring special considerations outside of the norm.

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Abbreviations

3D-MSI:

Three-dimensional mass spectrometry imaging

9-AA:

9-Aminoacridine

AP-MALDI:

Atmospheric pressure matrix-assisted laser desorption ionization

CHCA (or HCCA):

α-Cyano-4-hydroxycinnamic acid

Cer:

Ceramide

DAN:

1,5-Diaminonapthalene

DESI:

Desorption electrospray ionization

DHAP:

2,5-Dihydroxyacetophenone

DHB:

2,5-Dihydroxybenzoic acid

DMAN:

1,8-Bis(dimethylamino)naphthalene

FA:

Fatty acid

FFPE:

Formalin-fixed paraffin embedded

fNPs:

Functional iron nanoparticles

FT:

Fourier transform

FTICR:

Fourier transform ion cyclotron resonance

FT-IR:

Fourier transform infrared spectroscopy

HCA:

Hierarchical cluster analysis

Hex:

Hexose

IR:

Infrared

IR-MALDI:

Infrared matrix-assisted laser desorption ionization

ITO:

Indium tin oxide

kMSI:

Kinetic mass spectrometry imaging

LDI:

Laser desorption ionization

MALDI:

Matrix-assisted laser desorption ionization

MIPC:

Ceramide phosphoinositol

MRI:

Magnetic resonance imaging

MS:

Mass spectrometry

MSn :

Multistage tandem mass spectrometry

MSI:

Mass spectrometry imaging

MS/MS:

Tandem mass spectrometry

m/z :

Mass-to-charge ratio

NIMS:

Nanostructure-initiator mass spectrometry

OCT:

Optimal cutting temperature

PA:

Phosphatidic acid

PCA:

Principal component analysis

PC:

Phosphatidylcholine

PE:

Phosphatidylethanolamine

PG:

Phosphatidylglycerol

PI:

Phosphatidylinositol

PS:

Phosphatidylserine

ROI:

Region of interest

RP:

Resolving power

SIMS:

Secondary ion mass spectrometry

TIC:

Total ion chromatogram

TOF:

Time of flight

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Acknowledgments

Mr Dinaiz Thinagaran, University of Melbourne, for literature search and collation of references. Ms Sydney Currier, University of Toronto, for sample preparation of kangaroo brain images. Mr Sean O’Callaghan, Metabolomics Australia, for bioinformatics support. Metabolomics Australia, a NCRIS initiative under Bioplatforms Australia Pty Ltd. Dr Eivind Undheim, University of Queensland for Thereuopoda longicornis venom gland sample. Dr Anthony Carroll, Griffith University, for the Stylissa flabellata sample.

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  1. Metabolomics Australia, School of BioSciences, The University of Melbourne, Melbourne, VIC, 3010, Australia

    Berin A. Boughton

  2. Mater Research Institute, The University of Queensland, St Lucia, QLD, Australia

    Brett Hamilton

  3. Pathology Department, Mater Health Services, South Brisbane, QLD, Australia

    Brett Hamilton

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  1. Berin A. Boughton
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  1. University of Campinas (UNICAMP) Institute of Chemistry Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), Campinas, Brazil

    Alessandra Sussulini

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Boughton, B.A., Hamilton, B. (2017). Spatial Metabolite Profiling by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Imaging. In: Sussulini, A. (eds) Metabolomics: From Fundamentals to Clinical Applications. Advances in Experimental Medicine and Biology(), vol 965. Springer, Cham. https://doi.org/10.1007/978-3-319-47656-8_12

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