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Use of Commercial Enzymes to Boost On-Site Enzyme Efficiency

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Renewable Biofuels

Part of the book series: SpringerBriefs in Applied Sciences and Technology ((BRIEFSAPPLSCIENCES))

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Abstract

In-house production of cellulases from filamentous fungi is widely used, but their hydrolytic efficiency compared to commercial enzymes is limited. We studied the effect of supplementing in-house cellulases produced by Trichoderma reesei RUT-C30 and a novel strain Aspergillus saccharolyticus with different types of commercial enzymes for the efficient hydrolysis of wet-exploded loblolly pine. Cellic®Ctec 2, Cellic®Htec2, and Novozym 188 were used as the commercial base enzymes for supplementing the in-house produced enzymes. Compared to non-supplemented in-house enzyme preparation, commercial enzymes (Cellic®Ctec2) added in the same amount as FPU and CBU resulted in 68 % higher glucose yield using wet-exploded loblolly pine (WELP) at a 20 % DM concentration. Highest saccharification yield was achieved by supplementation of the in-house produced cellulases with Cellic®Htec2 compared to Cellic®Ctec2 and Novozym 188. Optimal glucose, xylose, and mannose yields, 85 %, 92 %, and 86 %, respectively, were achieved by using in-house enzymes (15 FPU/g cellulose) supplemented with commercial hemicellulase (7.5 FPU/g cellulose). These results showed that supplementing in-house enzymes with commercial enzymes can be advantageous and work for lowering the overall cost of enzymes in a biorefinery.

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Authors and Affiliations

  1. Black & Veatch Corporation, Kansas City, Missouri, USA

    Vandana Rana

  2. E. I. du Pont de Nemours and Company, Overland Park, Kansas, USA

    Diwakar Rana

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  1. Vandana Rana
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  2. Diwakar Rana
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Rana, V., Rana, D. (2017). Use of Commercial Enzymes to Boost On-Site Enzyme Efficiency. In: Renewable Biofuels. SpringerBriefs in Applied Sciences and Technology. Springer, Cham. https://doi.org/10.1007/978-3-319-47379-6_4

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  • DOI: https://doi.org/10.1007/978-3-319-47379-6_4

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