Abstract
Cellulase production by two filamentous fungi Trichoderma reesei RUT-C30 and novel fungal strain, Aspergillus saccharolyticus, on pretreated corn stover was investigated. Cellulase production was followed by the hydrolysis of wet-exploded corn stover (WECS) and wet-exploded loblolly pine (WELP) by on-site produced enzyme cocktail containing cellulase from T. reesei RUT-C30 and β-glucosidase from A. saccharolyticus. The sugar yields by using the on-site enzyme cocktail were compared with the commercial enzyme preparations, Celluclast 1.5 L and Novozym 188 at two substrate concentrations, 5 and 10 % (w/w), and enzyme loading at 5 and 15 FPU/g glucan for WECS and WELP. Highest sugar yields were obtained at 5 % (w/w) substrate concentration and 15 FPU/g glucan for both feedstocks, WECS and WELP. Glucose yields of 81 and 88 % were obtained from on-site and commercial enzymes, respectively, from WECS, and 55 and 58 % were achieved from on-site and commercial enzymes, respectively, from WELP.
The original version of this chapter was revised. An erratum to this chapter can be found at DOI 10.1007/978-3-319-47379-6_6
Reprinted from Bioresource Technology, Vol. 154, Rana V, Eckard AD, Teller P, Ahring BK, On-site enzymes produced from Trichoderma reesei RUT-C30 and Aspergillus saccharolyticus for hydrolysis of wet exploded corn stover and loblolly pine, PP. 282–289, Copyright 2014, with permission from Elsevier.
An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-3-319-47379-6_6
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Aden, A., & Foust, T. (2009). Technoeconomic analysis of the dilute sulfuric acid and enzymatic hydrolysis process for the conversion of corn stover to ethanol. Cellulose, 16(4), 535–545. doi:10.1007/s10570-009-9327-8.
Alvira, P., Gyalai-Korpos, M., Barta, Z., Oliva, J. M., Réczey, K., & Ballesteros, M. (2013). Production and hydrolytic efficiency of enzymes from Trichoderma reeseiRUTC30 using steam pretreated wheat straw as carbon source. Journal of Chemical Technology & Biotechnology, 88(6), 1150–1156. doi:10.1002/jctb.3955.
Bailey, M. J., Biely, P., & Poutanen, K. (1992). Interlaboratory testing of methods for assay of xylanase activity. Journal of Biotechnology, 23(3), 257–270. doi:10.1016/0168-1656(92)90074-J.
Bailey, M. J., & Tähtiharju, J. (2003). Efficient cellulase production by Trichoderma reesei in continuous cultivation on lactose medium with a computer-controlled feeding strategy. Applied Microbiology and Biotechnology, 62(2–3), 156–162. doi:10.1007/s00253-003-1276-9.
Bendig, C., & Weuster-Botz, D. (2012). Reaction engineering analysis of cellulase production with Trichoderma reesei RUT-C30 with intermittent substrate supply. Bioprocess and Biosystems Engineering, 1–8. doi:10.1007/s00449-012-0822-1.
Berlin, A., Balakshin, M., Gilkes, N., Kadla, J., Maximenko, V., Kubo, S., et al. (2006). Inhibition of cellulase, xylanase and β-glucosidase activities by softwood lignin preparations. Journal of Biotechnology, 125(2), 198–209. doi:10.1016/j.jbiotec.2006.02.021.
Bey, M., Berrin, J.-G., Poidevin, L., & Sigoillot, J.-C. (2011). Heterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccharification processes. Microbial Cell Factories, 10(1), 113.
Cantarella, M., Cantarella, L., Gallifuoco, A., Spera, A., & Alfani, F. (2004). Effect of inhibitors released during steam-explosion treatment of poplar wood on subsequent enzymatic hydrolysis and SSF. Biotechnology Progress, 20(1), 200–206. doi:10.1021/bp0257978.
Dutta, A., Dowe, N., Ibsen, K. N., Schell, D. J., & Aden, A. (2010). An economic comparison of different fermentation configurations to convert corn stover to ethanol using Z. mobilis and Saccharomyces. Biotechnology Progress, 26(1), 64–72. doi:10.1002/btpr.311.
Flachner, B., Brumbauer, A., & Reczey, K. (1999). Stabilization of beta-glucosidase in Aspergillus phoenicis QM 329 pellets - Evalutionary implications. Enzyme and Microbial Technology, 24(5), 362–367. doi:10.1016/s0141-0229(98)00133-1.
Foreman, P. K., Brown, D., Dankmeyer, L., Dean, R., Diener, S., Dunn-Coleman, N. S., et al. (2003). Transcriptional regulation of biomass-degrading enzymes in the filamentous fungus Trichoderma reesei. Journal of Biological Chemistry, 278(34), 31988–31997. doi:10.1074/jbc.M304750200.
García-Aparicio, M., Ballesteros, I., González, A., Oliva, J., Ballesteros, M., & Negro, M. (2006). Effect of inhibitors released during steam-explosion pretreatment of barley straw on enzymatic hydrolysis. Applied Biochemistry and Biotechnology, 129(1–3), 278–288. doi:10.1385/abab:129:1:278.
Ghose, T. (1987). Measurement of cellulase activities. Pure and Applied Chemistry, 59(2), 257–268.
Haki, G. D., & Rakshit, S. K. (2003). Developments in industrially important thermostable enzymes: A review. Bioresource Technology, 89(1), 17–34. doi:10.1016/S0960-8524(03)00033-6.
Hari Krishna, S., Sekhar Rao, K. C., Suresh Babu, J., & Srirami Reddy, D. (2000). Studies on the production and application of cellulase from Trichoderma reesei QM-9414. Bioprocess Engineering, 22(5), 467–470. doi:10.1007/s004490050760.
Juhász, T., Szengyel, Z., Réczey, K., Siika-Aho, M., & Viikari, L. (2005). Characterization of cellulases and hemicellulases produced by Trichoderma reesei on various carbon sources. Process Biochemistry, 40(11), 3519–3525. doi:10.1016/j.procbio.2005.03.057.
Kazi, F. K., Fortman, J. A., Anex, R. P., Hsu, D. D., Aden, A., Dutta, A., et al. (2010). Techno-economic comparison of process technologies for biochemical ethanol production from corn stover. Fuel, 89(Suppl 1), S20–S28. doi:10.1016/j.fuel.2010.01.001.
Kovács, K., Szakacs, G., & Zacchi, G. (2009). Comparative enzymatic hydrolysis of pretreated spruce by supernatants, whole fermentation broths and washed mycelia of Trichoderma reesei and Trichoderma atroviride. Bioresource Technology, 100(3), 1350–1357. doi:10.1016/j.biortech.2008.08.006.
Kumar, R., & Wyman, C. E. (2009). Effect of enzyme supplementation at moderate cellulase loadings on initial glucose and xylose release from corn stover solids pretreated by leading technologies. Biotechnology and Bioengineering, 102(2), 457–467. doi:10.1002/bit.22068.
Kurabi, A., Berlin, A., Gilkes, N., Kilburn, D., Bura, R., Robinson, J., et al. (2005). Enzymatic hydrolysis of steam-exploded and ethanol organosolv-pretreated Douglas-Fir by novel and commercial fungal cellulases. In B. Davison, B. Evans, M. Finkelstein, & J. McMillan (Eds.), Twenty-sixth symposium on biotechnology for fuels and chemicals (pp. 219–230). Totowa, NJ: Humana Press.
Lee, S. H., Doherty, T. V., Linhardt, R. J., & Dordick, J. S. (2009). Ionic liquid-mediated selective extraction of lignin from wood leading to enhanced enzymatic cellulose hydrolysis. Biotechnology and Bioengineering, 102(5), 1368–1376. doi:10.1002/bit.22179.
Martinez, D., Berka, R. M., Henrissat, B., Saloheimo, M., Arvas, M., Baker, S. E., et al. (2008). Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina). Nature Biotechnology, 26(5), 553–560. doi:10.1038/nbt1403.
Olsson, L., Christensen, T. M. I. E., Hansen, K. P., & Palmqvist, E. A. (2003). Influence of the carbon source on production of cellulases, hemicellulases and pectinases by Trichoderma reesei Rut C-30. Enzyme and Microbial Technology, 33(5), 612–619. doi:10.1016/S0141-0229(03)00181-9.
Rahikainen, J. L., Martin-Sampedro, R., Heikkinen, H., Rovio, S., Marjamaa, K., Tamminen, T., et al. (2013). Inhibitory effect of lignin during cellulose bioconversion: The effect of lignin chemistry on non-productive enzyme adsorption. Bioresource Technology, 133, 270–278. doi:10.1016/j.biortech.2013.01.075.
Rana, D., Rana, V., & Ahring, B. K. (2012). Producing high sugar concentrations from loblolly pine using wet explosion pretreatment. Bioresource Technology, 121, 61–67. doi:10.1016/j.biortech.2012.06.062.
Ryu, D. D. Y., & Mandels, M. (1980). Cellulases: Biosynthesis and applications. Enzyme and Microbial Technology, 2(2), 91–102. doi:10.1016/0141-0229(80)90063-0.
Sipos, B., Benkő, Z., Dienes, D., Réczey, K., Viikari, L., & Siika-aho, M. (2010). Characterisation of specific activities and hydrolytic properties of cell-wall-degrading enzymes produced by Trichoderma reesei Rut C30 on different carbon sources. Applied Biochemistry and Biotechnology, 161(1–8), 347–364. doi:10.1007/s12010-009-8824-4.
Sørensen, A., Teller, P., Lübeck, P., & Ahring, B. (2011). Onsite enzyme production during bioethanol production from biomass: Screening for suitable fungal strains. Applied Biochemistry and Biotechnology, 164(7), 1058–1070. doi:10.1007/s12010-011-9194-2.
Stockton, B. C., Mitchell, D. J., Grohmann, K., & Himmel, M. E. (1991). Optimumβ-D-glucosidase supplementation of cellulase for efficient conversion of cellulose to glucose. Biotechnology Letters, 13(1), 57–62. doi:10.1007/bf01033518.
Tabka, M. G., Herpoël-Gimbert, I., Monod, F., Asther, M., & Sigoillot, J. C. (2006). Enzymatic saccharification of wheat straw for bioethanol production by a combined cellulase xylanase and feruloyl esterase treatment. Enzyme and Microbial Technology, 39(4), 897–902. doi:10.1016/j.enzmictec.2006.01.021.
Um, B.-H., & Walsum, G. P. (2012). Effect of pretreatment severity on accumulation of major degradation products from dilute acid pretreated corn stover and subsequent inhibition of enzymatic hydrolysis of cellulose. Applied Biochemistry and Biotechnology, 168(2), 406–420. doi:10.1007/s12010-012-9784-7.
Wu, G., He, R., Jia, W., Chao, Y., & Chen, S. (2011). Strain improvement and process optimization of Trichderma reesei Rut C30 for enhanced cellulase production. Biofuels, 2(5), 545–555. doi:10.4155/bfs.11.124.
Zaldívar, M., Velásquez, J. C., Contreras, I., & Pérez, L. M. (2001). Trichoderma aureoviride 7-121, a mutant with enhanced production of lytic enzymes: Its potential use in waste cellulose degradation and/or biocontrol. Electronic Journal of Biotechnology, 4(3), 1–9.
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 2017 The Author(s)
About this chapter
Cite this chapter
Rana, V. (2017). Enzyme Production from Trichoderma reesei and Aspergillus Strain. In: Renewable Biofuels. SpringerBriefs in Applied Sciences and Technology. Springer, Cham. https://doi.org/10.1007/978-3-319-47379-6_3
Download citation
DOI: https://doi.org/10.1007/978-3-319-47379-6_3
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-319-47378-9
Online ISBN: 978-3-319-47379-6
eBook Packages: EngineeringEngineering (R0)