Application of Immunohistochemistry in Toxicologic Pathology of the Hematolymphoid System

  • Jerold E. RehgEmail author
  • Jerrold M. Ward
Part of the Molecular and Integrative Toxicology book series (MOLECUL)


Immunohistochemical analysis of formalin-fixed, paraffin-embedded (FFPE) tissues can evaluate the characteristics of immune cells in the same tissue that is routinely collected for toxicologic pathology assessment. This chapter will address which antigen markers are best used for assessing toxicologic effects in the hematolymphoid system and for diagnosing hematolymphoid neoplasms in FFPE tissues from mice and rats. These markers can be normally present on immune cells, which may increase or decrease in number or undergo a change in their level of marker expression after exposure to a toxin, toxicant, or immunomodulator.


Immunohistochemistry Hematolymphoid system Rodent Toxicology 



The authors thank Dorothy Bush for her excellent immunohistochemistry services, Debbie Cuffee for her secretarial assistance, and Keith Laycock for his editorial assistance.


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© Springer International Publishing AG 2017

Authors and Affiliations

  1. 1.Department of PathologySt. Jude Children’s Research HospitalMemphisUSA
  2. 2.Global VetPathologyMontgomeryUSA

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