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Application of Immunohistochemistry in Toxicologic Pathology of the Hematolymphoid System

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Book cover Immunopathology in Toxicology and Drug Development

Part of the book series: Molecular and Integrative Toxicology ((MOLECUL))

Abstract

Immunohistochemical analysis of formalin-fixed, paraffin-embedded (FFPE) tissues can evaluate the characteristics of immune cells in the same tissue that is routinely collected for toxicologic pathology assessment. This chapter will address which antigen markers are best used for assessing toxicologic effects in the hematolymphoid system and for diagnosing hematolymphoid neoplasms in FFPE tissues from mice and rats. These markers can be normally present on immune cells, which may increase or decrease in number or undergo a change in their level of marker expression after exposure to a toxin, toxicant, or immunomodulator.

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Acknowledgments

The authors thank Dorothy Bush for her excellent immunohistochemistry services, Debbie Cuffee for her secretarial assistance, and Keith Laycock for his editorial assistance.

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Authors and Affiliations

  1. Department of Pathology, St. Jude Children’s Research Hospital, MS 250, 262 Danny Thomas Place, Memphis, TN, USA

    Jerold E. Rehg

  2. Global VetPathology, Montgomery, MD, USA

    Jerrold M. Ward

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  1. Jerold E. Rehg
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  2. Jerrold M. Ward
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Correspondence to Jerold E. Rehg .

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  1. Charles River Laboratories, Inc. , Hillsborough, North Carolina, USA

    George A. Parker

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Rehg, J.E., Ward, J.M. (2017). Application of Immunohistochemistry in Toxicologic Pathology of the Hematolymphoid System. In: Parker, G. (eds) Immunopathology in Toxicology and Drug Development. Molecular and Integrative Toxicology. Humana Press, Cham. https://doi.org/10.1007/978-3-319-47377-2_10

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